LOCUS       sequence-164551-        4806 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing ATF6 , part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4806)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 4806)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_ATF6_WT
FEATURES             Location/Qualifiers
     source          1..4806
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             671..2683
                     /codon_start=1
                     /product="human activating transcription factor 6, an 
                     endoplasmic reticulum (ER) protein involved in the unfolded
                     protein response"
                     /label=ATF6
                     /note="Accumulation of misfolded proteins in the ER leads 
                     to proteolytic cleavage that releases the cytosolic domain 
                     of ATF6, which enters the nucleus and acts as a 
                     transcription factor."
                     /translation="MGEPAGVAGTMESPFSPGLFHRLDEDWDSALFAELGYFTDTDELQ
                     LEAANETYENNFDNLDFDLDLLPWESDIWDINNQICTVKDIKAEPQPLSPASSSYSVSS
                     PRSVDSYSSTQHVPEELDLSSSSQMSPLSLYGENSNSLSSPEPLKEDKPVTGSRNKTEN
                     GLTPKKKIQVNSKPSIQPKPLLLPAAPKTQTNSSVPAKTIIIQTVPTLMPLAKQQPIIS
                     LQPAPTKGQTVLLSQPTVVQLQAPGVLPSAQPVLAVAGGVTQLPNHVVNVVPAPSANSP
                     VNGKLSVTKPVLQSTMRNVGSDIAVLRRQQRIIKNRESACQSRKKKKEYMLGLEARLKA
                     ALSENEQLKKENGTLKRQLDEVVSENQRLKVPSPKRRVVCVMIVLAFIILNYGPMSMLE
                     QDSRRMNPSVSPANQRRHLLGFSAKEAQDTSDGIIQKNSYRYDHSVSNDKALMVLTEEP
                     LLYIPPPPCQPLINTTESLRLNHELRGWVHRHEVERTKSRRMTNNQQKTRILQGALEQG
                     SNSQLMAVQYTETTSSISRNSGSELQVYYASPRSYQDFFEAIRRRGDTFYVVSFRRDHL
                     LLPATTHNKTTRPKMSIVLPAININENVINGQDYEVMMQIDCQVMDTRILHIKSSSVPP
                     YLRDQQRNQTNTFFGSPPAATEATHVVSTIPESLQ"
     protein_bind    complement(2685..2784)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2801..2820)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2802..2820)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2825..2841)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2825..2841)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             3272..4063
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      4156..4744
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4645..4664
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agttggcacc atgggggagc cggctggggt tgccggcacc atggagtcac cttttagccc
      721 gggactcttt cacaggctgg atgaagattg ggattctgct ctctttgctg aactcggtta
      781 tttcacagac actgatgagc tgcaattgga agcagcaaat gagacgtatg aaaacaattt
      841 tgataatctt gattttgatt tggatttgtt accttgggag tcagacattt gggacatcaa
      901 caaccaaatc tgtacagtta aagatattaa ggcagaaccc cagccacttt ctccagcctc
      961 ctcaagttat tcagtctcat ctcctcggtc agtggactct tattcttcaa ctcagcatgt
     1021 tcctgaggag ttggatttgt cttctagttc tcagatgtct cccctttcct tatatggtga
     1081 aaactctaat agtctctctt caccggagcc actgaaggaa gataagcctg tcactggttc
     1141 taggaacaag actgaaaatg gactgactcc aaagaaaaaa attcaggtga attcaaaacc
     1201 ttcaattcag cccaagcctt tattgcttcc agcagcaccc aagactcaaa caaactccag
     1261 tgttccagca aaaaccatca ttattcagac agtaccaacg cttatgccat tggcaaagca
     1321 gcaaccaatt atcagtttac aacctgcacc cactaaaggc cagacggttt tgctgtctca
     1381 gcctactgtg gtacaacttc aagcacctgg agttctgccc tctgctcagc cagtccttgc
     1441 tgttgctggg ggagtcacac agctccctaa tcacgtggtg aatgtggtac cagccccttc
     1501 agcgaatagc ccagtgaatg gaaaactttc cgtgactaaa cctgtcctac aaagtaccat
     1561 gagaaatgtc ggttcagata ttgctgtgct aaggagacag caacgtataa taaaaaatcg
     1621 agaatccgct tgtcagtctc gcaagaagaa gaaagaatat atgctagggt tagaggcgag
     1681 attaaaggct gccctctcag aaaacgagca actgaagaaa gaaaatggaa cactgaagcg
     1741 gcagctggat gaagttgtgt cagagaacca gaggcttaaa gtccctagtc caaagcgaag
     1801 agttgtctgt gtgatgatag tattggcatt tataatactg aactatggac ctatgagcat
     1861 gttggaacag gattccagga gaatgaatcc tagtgtgagc cctgcaaatc aaaggaggca
     1921 ccttctagga ttttctgcta aagaggcaca ggacacatca gatggtatta tccagaaaaa
     1981 cagctacaga tatgatcatt ctgtttcaaa tgacaaagcc ctgatggtgc taactgaaga
     2041 accattgctt tacattcccc cacctccttg tcagccccta attaatacaa cagagtctct
     2101 caggttaaat catgaacttc gaggatgggt tcatagacat gaagtagaaa ggaccaagtc
     2161 aagaagaatg acaaataatc aacagaaaac ccgtattctt cagggtgctc tggaacaggg
     2221 ctcaaattct cagctgatgg ctgttcaata cacagaaacc actagtagta tcagcaggaa
     2281 ctcagggagt gagctacaag tgtattatgc ttcacccaga agttatcaag acttttttga
     2341 agccatccgc agaaggggag acacatttta tgttgtgtca tttcgaaggg atcacctgct
     2401 gttaccagct accacccata acaagaccac aagaccaaaa atgtcaattg tgttaccagc
     2461 aataaacata aatgagaatg tgatcaatgg gcaggactac gaagtgatga tgcagattga
     2521 ctgtcaggtg atggacacca ggatcctcca tatcaaaagt tcgtcggttc ctccttacct
     2581 ccgagatcag cagaggaatc aaaccaacac cttctttggc tcccctcccg cagccacaga
     2641 ggcaacccac gttgtcagca ccatccctga gtcattacaa tagttgccaa ctttcttgta
     2701 caaagttggc attataagaa agcattgctt atcaatttgt tgcaacgaac aggtcactat
     2761 cagtcaaaat aaaatcatta tttgccatcc agctgatatc ccctatagtg agtcgtatta
     2821 catggtcata gctgtttcct ggcagctctg gcccgtgtct caaaatctct gatgttacat
     2881 tgcacaagat aaaaatatat catcatgcct cctctagacc agccaggaca gaaatgcctc
     2941 gacttcgctg ctacccaagg ttgccgggtg acgcacaccg tggaaacgga tgaaggcacg
     3001 aacccagtgg acataagcct gttcggttcg taagctgtaa tgcaagtagc gtatgcgctc
     3061 acgcaactgg tccagaacct tgaccgaacg cagcggtggt aacggcgcag tggcggtttt
     3121 catggcttgt tatgactgtt tttttggggt acagtctatg cctcgggcat ccaagcagca
     3181 agcgcgttac gccgtgggtc gatgtttgat gttatggagc agcaacgatg ttacgcagca
     3241 gggcagtcgc cctaaaacaa agttaaacat tatgagggaa gcggtgatcg ccgaagtatc
     3301 gactcaacta tcagaggtag ttggcgtcat cgagcgccat ctcgaaccga cgttgctggc
     3361 cgtacatttg tacggctccg cagtggatgg cggcctgaag ccacacagtg atattgattt
     3421 gctggttacg gtgaccgtaa ggcttgatga aacaacgcgg cgagctttga tcaacgacct
     3481 tttggaaact tcggcttccc ctggagagag cgagattctc cgcgctgtag aagtcaccat
     3541 tgttgtgcac gacgacatca ttccgtggcg ttatccagct aagcgcgaac tgcaatttgg
     3601 agaatggcag cgcaatgaca ttcttgcagg tatcttcgag ccagccacga tcgacattga
     3661 tctggctatc ttgctgacaa aagcaagaga acatagcgtt gccttggtag gtccagcggc
     3721 ggaggaactc tttgatccgg ttcctgaaca ggatctattt gaggcgctaa atgaaacctt
     3781 aacgctatgg aactcgccgc ccgactgggc tggcgatgag cgaaatgtag tgcttacgtt
     3841 gtcccgcatt tggtacagcg cagtaaccgg caaaatcgcg ccgaaggatg tcgctgccga
     3901 ctgggcaatg gagcgcctgc cggcccagta tcagcccgtc atacttgaag ctagacaggc
     3961 ttatcttgga caagaagaag atcgcttggc ctcgcgcgca gatcagttgg aagaatttgt
     4021 ccactacgtg aaaggcgaga tcaccaaggt agtcggcaaa taaccctcga gccacccatg
     4081 accaaaatcc cttaacgtga gttacgcgtc gttccactga gcgtcagacc ccgtagaaaa
     4141 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     4201 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     4261 gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta
     4321 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     4381 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
     4441 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     4501 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
     4561 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
     4621 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     4681 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
     4741 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
     4801 catgtt
//
