LOCUS       sequence-164552-        5325 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing AXIN2 , part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5325)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 5325)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_AXIN2_WT
FEATURES             Location/Qualifiers
     source          1..5325
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             2075..2092
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     protein_bind    complement(3204..3303)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(3320..3339)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3321..3339)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3344..3360)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3344..3360)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             3791..4582
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      4675..5263
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5164..5183
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agttggcacc atgagtagcg ctatgttggt gacttgcctc ccggacccca gcagcagctt
      721 ccgtgaggat gccccgcggc ccccagtgcc aggggaagaa ggggagaccc caccgtgtca
      781 gccaggggtg ggcaagggcc aggtcaccaa acccatgtct gtctcttcca acaccaggcg
      841 gaacgaagat gggttggggg agccggaggg gcgggcatct ccggattccc ctctgacccg
      901 gtggaccaag tccttacact ccttattggg cgatcaagac ggtgcttacc tgttccgaac
      961 tttcctggag agggagaaat gcgtggatac cttagacttc tggtttgcct gcaatggatt
     1021 caggcagatg aacctgaagg ataccaaaac tttacgagta gccaaagcga tctacaaaag
     1081 gtacattgag aacaacagca ttgtctccaa gcagctgaag cctgccacca agacctacat
     1141 aagagatggc atcaagaagc agcagattga ttccatcatg tttgaccagg cgcagaccga
     1201 gatccagtcg gtgatggagg aaaatgccta ccagatgttt ttgacttctg atatatacct
     1261 cgaatatgtg aggagtgggg gagaaaacac agcttacatg agtaatgggg gactcgggag
     1321 cctaaaggtc gtgtgtggct atctccccac cttgaatgaa gaagaggagt ggacttgtgc
     1381 cgacttcaag tgcaaacttt cgccaaccgt ggttggcttg tccagcaaaa ctctgagggc
     1441 cacggcgagt gtgaggtcca cggaaactgt tgacagtgga tacaggtcct tcaagaggag
     1501 cgatcctgtt aatccttatc acataggttc tggctatgtc tttgcaccag ccaccagcgc
     1561 caacgacagt gagatatcca gtgatgcgct gacggatgat tccatgtcca tgacggacag
     1621 cagtgtagat ggaattcctc cttatcgtgt gggcagtaag aaacagctcc agagagaaat
     1681 gcatcgcagt gtgaaggcca atggccaagt gtctctacct catttcccga gaacccaccg
     1741 cctgcccaag gagatgaccc ccgtggaacc cgccaccttt gcagctgagc tgatctcgag
     1801 gctggaaaag ctgaagctgg agttggagag ccgccacagc ctggaggagc gcctgcagca
     1861 gatccgagag gatgaagaga gagagggctc cgagctcaca ctcaattcgc gggagggggc
     1921 gcccacgcag caccccctct ccctactgcc ctccggcagc tacgaggaag acccgcagac
     1981 gatactggac gatcacctgt ccagggtcct caagacccct ggctgccagt ctccgggcgt
     2041 aggccgctat agccctcgct cccgctcccc ggaccaccac caccaccacc attcgcagta
     2101 ccactccctg ctcccgcccg gtggcaagct gcctcccgcg gccgcctcgc cgggcgcctg
     2161 ccccctcctc gggggcaaag gctttgtgac caagcagacg acgaagcatg tccaccacca
     2221 ctacatccac caccatgccg tccccaagac caaggaggag atcgaggcgg aggccacgca
     2281 gcgggtgcac tgcttctgcc ctgggggcag cgagtattac tgctactcga aatgcaaaag
     2341 ccactccaag gctccggaaa ccatgcccag cgagcagttt ggcggcagca gaggcagtac
     2401 cttgcccaaa cgcaatggga aaggcacgga gccgggcctg gccctgcccg ccagggaagg
     2461 aggggccccc ggcggagctg gggccctgca gcttccccgg gaggaaggag acaggtcgca
     2521 ggatgtctgg cagtggatgc tggagagtga gcggcagagc aagcccaagc cccatagtgc
     2581 ccaaagcaca aaaaaggcct accccttgga gtctgcccgc tcgtctccag gcgaacgagc
     2641 cagccggcac catctgtggg ggggcaacag cgggcacccc cgcaccaccc cccgtgccca
     2701 cctgttcacc caggaccctg cgatgcctcc cctgacccca cccaacacgc tggctcagct
     2761 ggaggaggcc tgtcgcaggc tagctgaggt gtcgaagccc ccaaagcagc ggtgctgtgt
     2821 ggccagtcag cagagggaca ggaatcattc ggccactgtt cagacgggag ccacaccctt
     2881 ctccaatcca agcctggctc cagaagatca caaagagcca aagaaactgg caggtgtcca
     2941 cgcgctccag gccagtgagt tggttgtcac ttactttttc tgtggggaag aaattccata
     3001 ccggaggatg ctgaaggctc agagcttgac cctgggccac tttaaagagc agctcagcaa
     3061 aaagggaaat tataggtatt acttcaaaaa agcaagcgat gagtttgcct gtggagcggt
     3121 gtttgaggag atctgggagg atgagacggt gctcccgatg tatgaaggcc ggattctggg
     3181 caaagtggag cggatcgatt gattgccaac tttcttgtac aaagttggca ttataagaaa
     3241 gcattgctta tcaatttgtt gcaacgaaca ggtcactatc agtcaaaata aaatcattat
     3301 ttgccatcca gctgatatcc cctatagtga gtcgtattac atggtcatag ctgtttcctg
     3361 gcagctctgg cccgtgtctc aaaatctctg atgttacatt gcacaagata aaaatatatc
     3421 atcatgcctc ctctagacca gccaggacag aaatgcctcg acttcgctgc tacccaaggt
     3481 tgccgggtga cgcacaccgt ggaaacggat gaaggcacga acccagtgga cataagcctg
     3541 ttcggttcgt aagctgtaat gcaagtagcg tatgcgctca cgcaactggt ccagaacctt
     3601 gaccgaacgc agcggtggta acggcgcagt ggcggttttc atggcttgtt atgactgttt
     3661 ttttggggta cagtctatgc ctcgggcatc caagcagcaa gcgcgttacg ccgtgggtcg
     3721 atgtttgatg ttatggagca gcaacgatgt tacgcagcag ggcagtcgcc ctaaaacaaa
     3781 gttaaacatt atgagggaag cggtgatcgc cgaagtatcg actcaactat cagaggtagt
     3841 tggcgtcatc gagcgccatc tcgaaccgac gttgctggcc gtacatttgt acggctccgc
     3901 agtggatggc ggcctgaagc cacacagtga tattgatttg ctggttacgg tgaccgtaag
     3961 gcttgatgaa acaacgcggc gagctttgat caacgacctt ttggaaactt cggcttcccc
     4021 tggagagagc gagattctcc gcgctgtaga agtcaccatt gttgtgcacg acgacatcat
     4081 tccgtggcgt tatccagcta agcgcgaact gcaatttgga gaatggcagc gcaatgacat
     4141 tcttgcaggt atcttcgagc cagccacgat cgacattgat ctggctatct tgctgacaaa
     4201 agcaagagaa catagcgttg ccttggtagg tccagcggcg gaggaactct ttgatccggt
     4261 tcctgaacag gatctatttg aggcgctaaa tgaaacctta acgctatgga actcgccgcc
     4321 cgactgggct ggcgatgagc gaaatgtagt gcttacgttg tcccgcattt ggtacagcgc
     4381 agtaaccggc aaaatcgcgc cgaaggatgt cgctgccgac tgggcaatgg agcgcctgcc
     4441 ggcccagtat cagcccgtca tacttgaagc tagacaggct tatcttggac aagaagaaga
     4501 tcgcttggcc tcgcgcgcag atcagttgga agaatttgtc cactacgtga aaggcgagat
     4561 caccaaggta gtcggcaaat aaccctcgag ccacccatga ccaaaatccc ttaacgtgag
     4621 ttacgcgtcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
     4681 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
     4741 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
     4801 agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa
     4861 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
     4921 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
     4981 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
     5041 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
     5101 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
     5161 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
     5221 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
     5281 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgtt
//
