LOCUS       sequence-164569-        9955 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9955)
  AUTHORS   Scott KL, Nogueira C, Heffernan TP, van Doorn R, Dhakal S, Hanna JA,
            Min C, Jaskelioff M, Xiao Y, Wu CJ, Cameron LA, Perry SR, Zeid R, 
            Feinberg T, Kim M, Vande Woude G, Granter SR, Bosenberg M, Chu GC, 
            DePinho RA, Rimm DL, Chin L
  TITLE     Proinvasion metastasis drivers in early-stage melanoma are 
            oncogenes.
  JOURNAL   Cancer Cell. 2011 Jul 12;20(1):92-103.
  PUBMED    21741599
REFERENCE   2  (bases 1 to 9955)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cancer 
            Cell."; date: "2011-07-12"; volume: "20(1)"; pages: "92-103"
COMMENT     Sequence Label: pLenti6/V5-DEST-NCAPH
FEATURES             Location/Qualifiers
     source          1..9955
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        3..229
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             230..410
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    457..582
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1075..1308
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1493..1537
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1804..1921
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        1944..2247
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2248..2451
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2402..2422
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2448..2472
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    2568..2592
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(4816..4840)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             4893..4934
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    4958..5546
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5011..5031)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5429..5440)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        5561..5877
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5728..5863
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        5925..5972
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             5991..6389
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     LTR             6476..6709
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6781..6902
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6818..6837)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6872..6891
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6942..7077
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7097..7116)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7098..7116)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7126..7143)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7126..7142)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7135..7157)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7284..7739
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7371..7390)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7581..7602
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7765..7869
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7870..7938,7939..8730)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8088..8107)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8901..9489
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9390..9409
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9643..9660
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9777..9798
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9813..9818,9819..9836,9837..9843)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9851..9867
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9856..9878
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9875..9891
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9875..9891
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9910..9930
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        9912..9930
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca
       61 tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga
      121 tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt
      181 gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg
      241 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
      301 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
      361 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
      421 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
      481 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
      541 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
      601 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
      661 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
      721 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
      781 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
      841 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
      901 aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga
      961 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
     1021 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
     1081 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct
     1141 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
     1201 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
     1261 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg
     1321 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
     1381 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgataag cttgggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga
     1981 ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca
     2041 atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca
     2101 gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg
     2161 cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc
     2221 tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt
     2281 ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt
     2341 ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg
     2401 acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc tcgtttagtg
     2461 aaccgtcaga tcgcctggag acgccatcca cgctgttttg acctccatag aagacaccga
     2521 ctctagagga tccactagtc cagtgtggtg gaattctgca gatatcaaca agtttgtaca
     2581 aaaaagttgg catgggacct cccggcccag cactgccagc cacaatgaat aactcttctt
     2641 cagagacgcg aggacacccc cacagtgcct cctctccttc agagcgtgtg ttcccgatgc
     2701 ccctgcccag gaaggcgcct ctcaatattc ctggcacccc agtcctcgaa gactttcctc
     2761 agaatgacga tgagaaggag cggctgcagc ggaggcgctc gagggtcttt gatctgcagt
     2821 tcagcactga ctcacctcgc ttattggcct ccccctccag caggagtatt gacatttcag
     2881 ctactatccc caagtttaca aacacgcaga ttacggaaca ttactccacc tgtatcaaac
     2941 tgtccactga aaataaaatc actaccaaga atgcttttgg tttgcacttg attgatttta
     3001 tgtcagagat tcttaaacag aaagacaccg aaccaaccaa ctttaaagtg gctgcgggta
     3061 ctctggatgc cagcaccaag atctatgctg tgcgcgtgga tgccgtccat gccgatgtat
     3121 acagagtcct tggggggctg ggcaaagatg caccgtcttt ggaagaagta gaaggccatg
     3181 ttgctgatgg aagtgctact gaaatgggaa caaccaaaaa ggctgtaaag ccaaagaaga
     3241 agcacttaca cagaactatt gagcagaaca taaacaacct caatgtctcc gaagcagatc
     3301 ggaagtgtga gattgatccc atgtttcaga agacagcagc ctcatttgat gagtgcagca
     3361 cagcaggggt gtttctgtcc actctccact gccaggacta cagaagtgaa ctgctgtttc
     3421 cctctgatgt ccagactctc tccacgggag aacctctcga gttgccagag ttaggttgtg
     3481 tagaaatgac agatttaaaa gcgcccttgc agcagtgtgc agaagatcgc cagatctgcc
     3541 cttccctggc cgggttccag tttacacagt gggacagtga aacacataat gagtctgtgt
     3601 cggccctggt agacaagttt aagaagaatg accaggtatt tgacatcaat gctgaagttg
     3661 acgagagtga ctgtggagac ttccccgatg ggtccctggg ggatgacttt gatgccaacg
     3721 atgaacctga ccacaccgca gttggggatc atgaagagtt caggagctgg aaggagccct
     3781 gccaggttca gagctgccag gaagaaatga tttcccttgg ggatggagac atcaggacca
     3841 tgtgccccct tctgtctatg aaacctggag aatattctta tttcagtcct cggaccatgt
     3901 cgatgtgggc tggcccggat cactggcgct ttaggcctcg acgcaaacaa gatgctcctt
     3961 cccaatcaga aaacaaaaag aagagtacaa aaaaagattt tgaaattgac tttgaagatg
     4021 atattgactt tgatgtatat tttagaaaaa caaaggctgc tactattctg accaagtcca
     4081 ctttggagaa ccagaattgg agagctacca cccttcctac agatttcaac tacaatgttg
     4141 acactctggt ccagcttcac ctcaaaccag gcaccaggtt acttaagatg gcccagggcc
     4201 atagggcaga gactgagcat tatgaagaaa ttgaagacta tgattacaac aaccctaacg
     4261 acacctccaa cttttgccct ggattacagg ctgctgacag tgatgatgaa gatttggatg
     4321 acttatttgt gggacctgtt gggaactctg acctctcacc ttatccttgc catccaccta
     4381 agacagcaca acagaatggt gacactccag aagcccaagg attagacatc acaacatatg
     4441 gggagtcaaa cttggtagct gagcctcaga aggtaaataa aattgaaatt cactatgcca
     4501 agactgccaa aaagatggac atgaagaaac tgaagcagag catgtggagt ctgctgacag
     4561 cgctctccgg aaaggaggca gatgcagagg caaaccacag ggaagctgga aaagaagcgg
     4621 ccctggcaga agtggctgac gagaagatgc ttagcgggct cacgaaggac ctgcagagga
     4681 gcctgccccc tgtcatggct cagaacctct ccatacctct ggcttttgcc tgtctcctac
     4741 atttagccaa tgaaaagaat ctaaaactgg aaggaacaga ggacctttct gatgttcttg
     4801 tgaggcaagg agattgccca actttcttgt acaaagtggt tgatatccag cacagtggcg
     4861 gccgctcgag tctagagggc ccgcggttcg aaggtaagcc tatccctaac cctctcctcg
     4921 gtctcgattc tacgcgtacc ggttagtaat gatcgacaat caacctctgg attacaaaat
     4981 ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc
     5041 tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt
     5101 gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg
     5161 cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg
     5221 tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc
     5281 cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt
     5341 gttgtcgggg aagctgacgt cctttccatg gctgctcgcc tgtgttgcca cctggattct
     5401 gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg
     5461 cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg
     5521 gatctccctt tgggccgcct ccccgcctgg cgatggtacc ggtgtggaaa gtccccaggc
     5581 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga
     5641 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca
     5701 accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat
     5761 tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc cgcctctgcc
     5821 tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag
     5881 ctcccgggag cttgtatatc cattttcgga tctgatcagc acgtgttgac aattaatcat
     5941 cggcatagta tatcggcata gtataatacg acaaggtgag gaactaaacc atggccaagc
     6001 ctttgtctca agaagaatcc accctcattg aaagagcaac ggctacaatc aacagcatcc
     6061 ccatctctga agactacagc gtcgccagcg cagctctctc tagcgacggc cgcatcttca
     6121 ctggtgtcaa tgtatatcat tttactgggg gaccttgtgc agaactcgtg gtgctgggca
     6181 ctgctgctgc tgcggcagct ggcaacctga cttgtatcgt cgcgatcgga aatgagaaca
     6241 ggggcatctt gagcccctgc ggacggtgcc gacaggtgct tctcgatctg catcctggga
     6301 tcaaagccat agtgaaggac agtgatggac agccgacggc agttgggatt cgtgaattgc
     6361 tgccctctgg ttatgtgtgg gagggctaag cacaattcga gctcggtacc tttaagacca
     6421 atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaaggg gggactggaa
     6481 gggctaattc actcccaacg aagacaagat ctgctttttg cttgtactgg gtctctctgg
     6541 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct
     6601 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt
     6661 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag tagtagttca
     6721 tgtcatctta ttattcagta tttataactt gcaaagaaat gaatatcaga gagtgagagg
     6781 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca
     6841 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct
     6901 tatcatgtct ggctctagct atcccgcccc taactccgcc catcccgccc ctaactccgc
     6961 ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg
     7021 aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag
     7081 ggacgtaccc aattcgccct atagtgagtc gtattacgcg cgctcactgg ccgtcgtttt
     7141 acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg cagcacatcc
     7201 ccctttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt
     7261 gcgcagcctg aatggcgaat gggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt
     7321 ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc
     7381 tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg
     7441 gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta
     7501 gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt
     7561 ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat
     7621 ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa
     7681 tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa atattaacgc ttacaattta
     7741 ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat
     7801 tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa
     7861 aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt
     7921 tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag
     7981 ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt
     8041 tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg
     8101 gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag
     8161 aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta
     8221 agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg
     8281 acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta
     8341 actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac
     8401 accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt
     8461 actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca
     8521 cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag
     8581 cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta
     8641 gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag
     8701 ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt
     8761 tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat
     8821 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta
     8881 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
     8941 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
     9001 tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag
     9061 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
     9121 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
     9181 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
     9241 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
     9301 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
     9361 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
     9421 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
     9481 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
     9541 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt
     9601 gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag
     9661 gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa
     9721 tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat
     9781 gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg
     9841 ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac
     9901 gccaagcgcg caattaaccc tcactaaagg gaacaaaagc tggagctgca agctt
//
