LOCUS       sequence-164570-        4965 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing DLL1 , part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4965)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 4965)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_DLL1_WT
FEATURES             Location/Qualifiers
     source          1..4965
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2844..2943)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2960..2979)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2961..2979)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2984..3000)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2984..3000)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             3431..4222
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      4315..4903
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4804..4823
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agttggcacc atgggcagtc ggtgcgcgct ggccctggcg gtgctctcgg ccttgctgtg
      721 tcaggtctgg agctctgggg tgttcgaact gaagctgcag gagttcgtca acaagaaggg
      781 gctgctgggg aaccgcaact gctgccgcgg gggcgcgggg ccaccgccgt gcgcctgccg
      841 gaccttcttc cgcgtgtgcc tcaagcacta ccaggccagc gtgtcccccg agccgccctg
      901 cacctacggc agcgccgtca cccccgtgct gggcgtcgac tccttcagtc tgcccgacgg
      961 cgggggcgcc gactccgcgt tcagcaaccc catccgcttc cccttcggct tcacctggcc
     1021 gggcaccttc tctctgatta ttgaagctct ccacacagat tctcctgatg acctcgcaac
     1081 agaaaaccca gaaagactca tcagccgcct ggccacccag aggcacctga cggtgggcga
     1141 ggagtggtcc caggacctgc acagcagcgg ccgcacggac ctcaagtact cctaccgctt
     1201 cgtgtgtgac gaacactact acggagaggg ctgctccgtt ttctgccgtc cccgggacga
     1261 tgccttcggc cacttcacct gtggggagcg tggggagaaa gtgtgcaacc ctggctggaa
     1321 agggccctac tgcacagagc cgatctgcct gcctggatgt gatgagcagc atggattttg
     1381 tgacaaacca ggggaatgca agtgcagagt gggctggcag ggccggtact gtgacgagtg
     1441 tatccgctat ccaggctgtc tccatggcac ctgccagcag ccctggcagt gcaactgcca
     1501 ggaaggctgg gggggccttt tctgcaacca ggacctgaac tactgcacac accataagcc
     1561 ctgcaagaat ggagccacct gcaccaacac gggccagggg agctacactt gctcttgccg
     1621 gcctgggtac acaggtgcca cctgcgagct ggggattgac gagtgtgacc ccagcccttg
     1681 taagaacgga gggagctgca cggatctcga gaacagctac tcctgtacct gcccacccgg
     1741 cttctacggc aaaatctgtg aattgagtgc catgacctgt gcggacggcc cttgctttaa
     1801 cgggggtcgg tgctcagaca gccccgatgg agggtacagc tgccgctgcc ccgtgggcta
     1861 ctccggcttc aactgtgaga agaaaattga ctactgcagc tcttcaccct gttctaatgg
     1921 tgccaagtgt gtggacctcg gtgatgccta cctgtgccgc tgccaggccg gcttctcggg
     1981 gaggcactgt gacgacaacg tggacgactg cgcctcctcc ccgtgcgcca acgggggcac
     2041 ctgccgggat ggcgtgaacg acttctcctg cacctgcccg cctggctaca cgggcaggaa
     2101 ctgcagtgcc cccgtcagca ggtgcgagca cgcaccctgc cacaatgggg ccacctgcca
     2161 cgagaggggc caccgctatg tgtgcgagtg tgcccgaggc tacgggggtc ccaactgcca
     2221 gttcctgctc cccgagctgc ccccgggccc agcggtggtg gacctcactg agaagctaga
     2281 gggccagggc gggccattcc cctgggtggc cgtgtgcgcc ggggtcatcc ttgtcctcat
     2341 gctgctgctg ggctgtgccg ctgtggtggt ctgcgtccgg ctgaggctgc agaagcaccg
     2401 gcccccagcc gacccctgcc ggggggagac ggagaccatg aacaacctgg ccaactgcca
     2461 gcgtgagaag gacatctcag tcagcatcat cggggccacg cagatcaaga acaccaacaa
     2521 gaaggcggac ttccacgggg accacagcgc cgacaagaat ggcttcaagg cccgctaccc
     2581 agcggtggac tataacctcg tgcaggacct caagggtgac gacaccgccg tcagggacgc
     2641 gcacagcaag cgtgacacca agtgccagcc ccagggctcc tcaggggagg agaaggggac
     2701 cccgaccaca ctcaggggtg gagaagcatc tgaaagaaaa aggccggact cgggctgttc
     2761 aacttcaaaa gacaccaagt accagtcggt gtacgtcata tccgaggaga aggatgagtg
     2821 cgtcatagca actgaggtgt aattgccaac tttcttgtac aaagttggca ttataagaaa
     2881 gcattgctta tcaatttgtt gcaacgaaca ggtcactatc agtcaaaata aaatcattat
     2941 ttgccatcca gctgatatcc cctatagtga gtcgtattac atggtcatag ctgtttcctg
     3001 gcagctctgg cccgtgtctc aaaatctctg atgttacatt gcacaagata aaaatatatc
     3061 atcatgcctc ctctagacca gccaggacag aaatgcctcg acttcgctgc tacccaaggt
     3121 tgccgggtga cgcacaccgt ggaaacggat gaaggcacga acccagtgga cataagcctg
     3181 ttcggttcgt aagctgtaat gcaagtagcg tatgcgctca cgcaactggt ccagaacctt
     3241 gaccgaacgc agcggtggta acggcgcagt ggcggttttc atggcttgtt atgactgttt
     3301 ttttggggta cagtctatgc ctcgggcatc caagcagcaa gcgcgttacg ccgtgggtcg
     3361 atgtttgatg ttatggagca gcaacgatgt tacgcagcag ggcagtcgcc ctaaaacaaa
     3421 gttaaacatt atgagggaag cggtgatcgc cgaagtatcg actcaactat cagaggtagt
     3481 tggcgtcatc gagcgccatc tcgaaccgac gttgctggcc gtacatttgt acggctccgc
     3541 agtggatggc ggcctgaagc cacacagtga tattgatttg ctggttacgg tgaccgtaag
     3601 gcttgatgaa acaacgcggc gagctttgat caacgacctt ttggaaactt cggcttcccc
     3661 tggagagagc gagattctcc gcgctgtaga agtcaccatt gttgtgcacg acgacatcat
     3721 tccgtggcgt tatccagcta agcgcgaact gcaatttgga gaatggcagc gcaatgacat
     3781 tcttgcaggt atcttcgagc cagccacgat cgacattgat ctggctatct tgctgacaaa
     3841 agcaagagaa catagcgttg ccttggtagg tccagcggcg gaggaactct ttgatccggt
     3901 tcctgaacag gatctatttg aggcgctaaa tgaaacctta acgctatgga actcgccgcc
     3961 cgactgggct ggcgatgagc gaaatgtagt gcttacgttg tcccgcattt ggtacagcgc
     4021 agtaaccggc aaaatcgcgc cgaaggatgt cgctgccgac tgggcaatgg agcgcctgcc
     4081 ggcccagtat cagcccgtca tacttgaagc tagacaggct tatcttggac aagaagaaga
     4141 tcgcttggcc tcgcgcgcag atcagttgga agaatttgtc cactacgtga aaggcgagat
     4201 caccaaggta gtcggcaaat aaccctcgag ccacccatga ccaaaatccc ttaacgtgag
     4261 ttacgcgtcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
     4321 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
     4381 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
     4441 agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa
     4501 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
     4561 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
     4621 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
     4681 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
     4741 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
     4801 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
     4861 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
     4921 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgtt
//
