LOCUS       sequence-164574-        4310 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gene Tagging vector HIS/CFP - Plasmid for yeast enhanced monomeric 
            CFP (yemCFP) labeling of endogenous proteins with a HIS selection 
            marker, originating from pSIVh (ID:81091).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4310)
  AUTHORS   Wosika V, Durandau E, Varidel C, Aymoz D, Schmitt M, Pelet S
  TITLE     New families of single integration vectors and gene tagging plasmids
            for genetic manipulations in budding yeast.
  JOURNAL   Mol Genet Genomics. 2016 Dec;291(6):2231-2240. Epub 2016 Sep 16.
  PUBMED    27637489
REFERENCE   2  (bases 1 to 4310)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Genet 
            Genomics."; date: "2016-12"; volume: "291(6)"; pages: "2231-2240. 
            Epub 2016 Sep 16"
COMMENT     Sequence Label: GTH-c
FEATURES             Location/Qualifiers
     source          1..4310
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(28..91)
                     /gene="bla"
                     /label=AmpR promoter
     promoter        118..136
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     CDS             168..884
                     /codon_start=1
                     /product="yeast-enhanced green fluorescent protein"
                     /label=yeGFP
                     /note="codons optimized for translation in S. cerevisiae 
                     and C. albicans"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYIYNSHNVYITADKQKNGIKA
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSRLSKDPNEKRDHMVLLE
                     FVTAAGITHGIDELYK"
     terminator      928..1115
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(983..1007)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     primer_bind     complement(1154..1170)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1154..1170)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     protein_bind    1171..1204
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             1706..2359
                     /codon_start=1
                     /gene="Schizosaccharomyces pombe his5"
                     /product="imidazoleglycerol-phosphate dehydratase, required
                     for histidine biosynthesis"
                     /label=S. pombe his5
                     /note="yeast auxotrophic marker; corresponds to S. 
                     cerevisiae HIS3"
                     /translation="MGRRAFVERNTNETKISVAIALDKAPLPEESNFIDELITSKHANQ
                     KGEQVIQVDTGIGFLDHMYHALAKHAGWSLRLYSRGDLIIDDHHTAEDTAIALGIAFKQ
                     AMGNFAGVKRFGHAYCPLDEALSRSVVDLSGRPYAVIDLGLKREKVGELSCEMIPHLLY
                     SFSVAAGITLHVTCLYGSNDHHRAESAFKSLAVAMRAATSLTGSSEVPSTKGVL"
     terminator      2365..2562
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     protein_bind    2586..2619
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        2620..2638
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(2667..2685)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      complement(2718..3306)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2798..2817)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3477..4268,4269..4310,1..27))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFFHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4100..4119
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta
       61 tcagggttat tgtctcatga gcggatacat atttgaagta tcgttcgaat taattaaaat
      121 taaccctcac taaaggggca tgcgcggccg ctctagaact aattaacatg tctaaaggtg
      181 aagaattatt cactggtgtt gtcccaattt tggttgaatt agatggtgat gttaatggtc
      241 acaaattttc tgtctccggt gaaggtgaag gtgatgctac ttacggtaaa ttgaccttaa
      301 aatttatttg tactactggt aaattgccag ttccatggcc aaccttagtc actactttaa
      361 cttggggtgt tcaatgtttt tctagatacc cagatcatat gaaacaacat gactttttca
      421 agtctgccat gccagaaggt tatgttcaag aaagaactat ttttttcaaa gatgacggta
      481 actacaagac cagagctgaa gtcaagtttg aaggtgatac cttagttaat agaatcgaat
      541 taaaaggtat tgattttaaa gaagatggta acattttagg tcacaaattg gaatacattt
      601 ataactctca caatgtttac atcactgctg acaaacaaaa gaatggtatc aaagctaact
      661 tcaaaattag acacaacatt gaagatggtt ctgttcaatt agctgaccat tatcaacaaa
      721 atactccaat tggtgatggt ccagtcttgt taccagacaa ccattactta tccactcaat
      781 ctagattatc caaagatcca aacgaaaaga gagaccacat ggtcttgtta gaatttgtta
      841 ctgctgctgg tattacccat ggtattgatg aattgtacaa ataaggatgc tagcatttta
      901 actcgagggc gcgccacttc taaataagcg aatttcttat gatttatgat ttttattatt
      961 aaataagtta taaaaaaaat aagtgtatac aaattttaaa gtgactctta ggttttaaaa
     1021 cgaaaattct tattcttgag taactctttc ctgtaggtca ggttgctttc tcaggtatag
     1081 tatgaggtcg ctcttattga ccacacctct accggcagat ccgctaggga taacagggta
     1141 atatagatcc atggtcatag ctgtttcctg ataacttcgt ataatgtatg ctatacgaag
     1201 ttatgtctgt aggcgaacct aaccggccaa ttttccaaga ccgctctgcc gctgagcttg
     1261 cacccgtgcc gctacacaac acccacacat ccgtaaaccc cccgccatcg gtatcaccaa
     1321 ggttgtctat gacgaggcgt acaagaggac gtgggggccg cctctccaat gtcaccaata
     1381 ggaaaaaaga aagaccgagt gcgagtttct gacagaagcc tcaagaattt tttttctttc
     1441 ttcgactatg ctggaggtag agatgatcga ggcagtagtc aagtatatat aaattaatta
     1501 gttccaccac cttcttttct tgtgttgctc ctttcgatgc tatttctggc tttttctaat
     1561 ttttttcatc gtttcttact ttcttcctaa tatataaatt cttttgcatt ttcattcttc
     1621 ctctccattt gttcattctc cttaaatttt ttttaggtct cctttctttc aagaaacaca
     1681 gtcatcgaac tataattaac taaacatggg taggagggct tttgtagaaa gaaatacgaa
     1741 cgaaacgaaa atcagcgttg ctatcgcttt ggacaaagct cccttacctg aagagtcgaa
     1801 ttttattgat gaacttataa cttccaaaca tgcaaaccaa aagggagaac aagtaatcca
     1861 agtagacacg ggaattggat tcttggatca catgtatcat gcactggcta aacatgcagg
     1921 ctggagctta cgactttact caagaggtga tttaatcatt gatgatcatc acacagcaga
     1981 agatactgct attgcacttg gtattgcatt caagcaggct atgggtaact ttgccggcgt
     2041 taaaagattt ggacatgctt attgtccact tgacgaagct ctttcaagaa gcgtagttga
     2101 tttgtcggga cggccctatg ctgttattga tttgggatta aagcgtgaaa aggttgggga
     2161 attgtcctgt gaaatgatcc ctcacttact atattccttt tcggtagctg ctggaattac
     2221 tttgcatgtt acctgcttat atggtagtaa tgaccatcat cgtgctgaaa gcgcttttaa
     2281 atctctggct gttgccatgc gcgcggctac aagtcttact ggaagttctg aagtcccaag
     2341 cacgaaggga gtgttgtaat cattactgac aataaaaaga ttcttgtttt caagaacttg
     2401 tcatttgtat agttttttta tattgtagtt gttctatttt aatcaaatgt tagcgtgatt
     2461 tatatttttt ttcgcctcga catcatctgc ccagatgcga agttaagtgc gcagaaagta
     2521 atatcatgcg tcaatcgtat gtgaatgctg gtcgctatac tgctgtcgat tcgatactaa
     2581 cgccgataac ttcgtataat gtatgctata cgaagttatt aatacgactc actatagggc
     2641 gaattggagc tccaattcgg acgtccccta tagtgagtcg tattattaat taattcgaag
     2701 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
     2761 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
     2821 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
     2881 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
     2941 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
     3001 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
     3061 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
     3121 ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct
     3181 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
     3241 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
     3301 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
     3361 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
     3421 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
     3481 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
     3541 gcctgactgc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
     3601 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag
     3661 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
     3721 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
     3781 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
     3841 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgaaa aaaagcggtt
     3901 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
     3961 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
     4021 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
     4081 tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc
     4141 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
     4201 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
     4261 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag
//
