LOCUS       sequence-164575-        4806 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing DVL1 , part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4806)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 4806)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_DVL1_WT
FEATURES             Location/Qualifiers
     source          1..4806
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2685..2784)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2801..2820)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2802..2820)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2825..2841)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2825..2841)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             3272..4063
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      4156..4744
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4645..4664
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agttggcacc atggcggaga ccaagattat ctaccacatg gacgaggagg agacgccgta
      721 cctggtcaag ctgcccgtgg cccccgagcg cgtcacgctg gccgacttca agaacgtgct
      781 cagcaaccgg cccgtgcacg cctacaaatt cttctttaag tccatggacc aggacttcgg
      841 ggtggtgaag gaggagatct ttgatgacaa tgccaagctt ccctgcttca acggccgcgt
      901 ggtctcctgg ctggtcctgg ctgagggtgc tcactcggat gcggggtccc agggcacgga
      961 cagccacaca gacctgcccc cgcctcttga gcggacaggc ggcatcgggg actcccggcc
     1021 cccctccttc cacccgaatg tggccagcag ccgtgacggg atggacaacg agacaggcac
     1081 ggagtccatg gtcagtcacc ggcgggagcg tgcccgacgc cggaaccgcg aggaggccgc
     1141 ccggaccaat gggcacccaa ggggagaccg acggcgggat gtggggctgc ccccagacag
     1201 cgcgtccacc gccctcagca gcgagcttga gtccagcagc tttgtggact cggacgagga
     1261 tggcagcacg agcaggctca gcagctccac ggagcagagc acctcatcca gactcatccg
     1321 gaagcacaaa cgccggcgga ggaagcagcg ccttcggcag gcggaccggg cctcctcctt
     1381 cagcagcata accgactcca ccatgtccct caacatcgtc actgtcacgc tcaacatgga
     1441 aagacatcac tttctgggca tcagcatcgt ggggcagagc aacgaccgtg gagacggcgg
     1501 catctacatt ggctccatca tgaagggcgg ggctgtggcc gctgacggcc gcatcgagcc
     1561 cggcgacatg ttgctgcagg tgaatgacgt gaactttgag aacatgagca atgacgatgc
     1621 cgtgcgggtg ctgcgggaga tcgtttccca gacggggccc atcagcctca ctgtggccaa
     1681 gtgctgggac ccaacgcccc gaagctactt caccgtccca cgggctgacc cggtgcggcc
     1741 catcgacccc gccgcctggc tgtcccacac ggcggcactg acaggagccc tgccccgcta
     1801 cgagctggaa gaggcgccgc tgacggtgaa gagtgacatg agcgccgtcg tccgggtcat
     1861 gcagctgcca gactcgggac tggagatccg cgaccgcatg tggctcaaga tcaccatcgc
     1921 caatgccgtc atcggggcgg acgtggtgga ctggctgtac acacacgtgg agggcttcaa
     1981 ggagcggcgg gaggcccgga agtacgccag cagcttgctg aagcacggct tcctgcggca
     2041 cacggtcaac aagatcacct tctccgagca gtgctactac gtcttcgggg atctctgcag
     2101 caatctcgcc accctgaacc tcaacagtgg ctccagtggg acttcggatc aggacacgct
     2161 ggccccgctg ccccacccgg ctgccccctg gcctctgggt cagggctacc cctaccagta
     2221 cccgggaccc ccaccctgct tcccgcctgc ctaccaggac ccgggcttta gctatggcag
     2281 cggcagcacc gggagtcagc agagtgaagg gagcaaaagc agtgggtcca cccggagcag
     2341 ccgccgggcc ccgggccgtg agaaggagcg tcgggcggcg ggagctgggg gcagtggcag
     2401 tgaatcggat cacacggcac cgagtggggt ggggagcagc tggcgagagc gtccggccgg
     2461 ccagctcagc cgtggcagca gcccacgcag tcaggcctcg gctaccgccc cggggctccc
     2521 cccgccccac cccacgacca aggcctatac agtggtgggg gggccacccg ggggaccccc
     2581 tgtccgggag ctggctgccg tccccccgga attgacaggc agccgccagt ccttccagaa
     2641 ggctatgggg aacccctgcg agttcttcgt ggacatcatg tgattgccaa ctttcttgta
     2701 caaagttggc attataagaa agcattgctt atcaatttgt tgcaacgaac aggtcactat
     2761 cagtcaaaat aaaatcatta tttgccatcc agctgatatc ccctatagtg agtcgtatta
     2821 catggtcata gctgtttcct ggcagctctg gcccgtgtct caaaatctct gatgttacat
     2881 tgcacaagat aaaaatatat catcatgcct cctctagacc agccaggaca gaaatgcctc
     2941 gacttcgctg ctacccaagg ttgccgggtg acgcacaccg tggaaacgga tgaaggcacg
     3001 aacccagtgg acataagcct gttcggttcg taagctgtaa tgcaagtagc gtatgcgctc
     3061 acgcaactgg tccagaacct tgaccgaacg cagcggtggt aacggcgcag tggcggtttt
     3121 catggcttgt tatgactgtt tttttggggt acagtctatg cctcgggcat ccaagcagca
     3181 agcgcgttac gccgtgggtc gatgtttgat gttatggagc agcaacgatg ttacgcagca
     3241 gggcagtcgc cctaaaacaa agttaaacat tatgagggaa gcggtgatcg ccgaagtatc
     3301 gactcaacta tcagaggtag ttggcgtcat cgagcgccat ctcgaaccga cgttgctggc
     3361 cgtacatttg tacggctccg cagtggatgg cggcctgaag ccacacagtg atattgattt
     3421 gctggttacg gtgaccgtaa ggcttgatga aacaacgcgg cgagctttga tcaacgacct
     3481 tttggaaact tcggcttccc ctggagagag cgagattctc cgcgctgtag aagtcaccat
     3541 tgttgtgcac gacgacatca ttccgtggcg ttatccagct aagcgcgaac tgcaatttgg
     3601 agaatggcag cgcaatgaca ttcttgcagg tatcttcgag ccagccacga tcgacattga
     3661 tctggctatc ttgctgacaa aagcaagaga acatagcgtt gccttggtag gtccagcggc
     3721 ggaggaactc tttgatccgg ttcctgaaca ggatctattt gaggcgctaa atgaaacctt
     3781 aacgctatgg aactcgccgc ccgactgggc tggcgatgag cgaaatgtag tgcttacgtt
     3841 gtcccgcatt tggtacagcg cagtaaccgg caaaatcgcg ccgaaggatg tcgctgccga
     3901 ctgggcaatg gagcgcctgc cggcccagta tcagcccgtc atacttgaag ctagacaggc
     3961 ttatcttgga caagaagaag atcgcttggc ctcgcgcgca gatcagttgg aagaatttgt
     4021 ccactacgtg aaaggcgaga tcaccaaggt agtcggcaaa taaccctcga gccacccatg
     4081 accaaaatcc cttaacgtga gttacgcgtc gttccactga gcgtcagacc ccgtagaaaa
     4141 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     4201 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     4261 gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta
     4321 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     4381 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
     4441 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     4501 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
     4561 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
     4621 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     4681 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
     4741 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
     4801 catgtt
//
