LOCUS       sequence-164576-        1041 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gene Tagging vector HIS/GFP - Plasmid for superfolder GFP (sfGFP) 
            labeling of endogenous proteins with a HIS selection marker, 
            originating from pSIVh (ID:81091).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1041)
  AUTHORS   Wosika V, Durandau E, Varidel C, Aymoz D, Schmitt M, Pelet S
  TITLE     New families of single integration vectors and gene tagging plasmids
            for genetic manipulations in budding yeast.
  JOURNAL   Mol Genet Genomics. 2016 Dec;291(6):2231-2240. Epub 2016 Sep 16.
  PUBMED    27637489
REFERENCE   2  (bases 1 to 1041)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Genet 
            Genomics."; date: "2016-12"; volume: "291(6)"; pages: "2231-2240. 
            Epub 2016 Sep 16"
COMMENT     Sequence Label: GTH-g
FEATURES             Location/Qualifiers
     source          1..1041
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        2..20
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     terminator      798..985
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(853..877)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     primer_bind     complement(1024..1040)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1024..1040)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 aaattaaccc tcactaaagg ggcatgcgcg gccgctctag aactatccaa gggtgaagag
       61 ctatttactg gggttgtacc cattttggta gaactggacg gagatgtaaa cggacataaa
      121 ttctctgtta gaggtgaggg cgaaggcgat gccaccaatg gtaaattgac tctgaagttt
      181 atatgcacta cgggtaaatt acctgttcct tggccaaccc tagtaacaac tttgacatat
      241 ggtgttcaat gtttctcaag atacccagac catatgaaaa ggcatgattt ctttaaaagt
      301 gctatgccag aaggctacgt gcaagagaga actatctcct ttaaggatga cggtacgtat
      361 aaaacacgag cagaagtgaa attcgaaggg gatacactag ttaatcgcat cgaattaaag
      421 ggtatagact ttaaggaaga tggtaatatt ctcggccata aacttgagta taatttcaac
      481 tcgcataatg tgtacattac agctgacaaa caaaagaacg gaattaaagc gaattttaaa
      541 atcaggcaca acgtcgaaga tgggtctgtt caacttgccg atcattatca gcaaaacacc
      601 cctattggtg atggtccagt cttgttaccc gataatcact acttaagcac acagtctaga
      661 ttgtcaaaag atccgaatga aaagcgtgat cacatggttt tattggaatt tgtcaccgct
      721 gcaggaataa ctcacggaat ggacgagctt tataagatgc tagcatttta actcgagggc
      781 gcgccacttc taaataagcg aatttcttat gatttatgat ttttattatt aaataagtta
      841 taaaaaaaat aagtgtatac aaattttaaa gtgactctta ggttttaaaa cgaaaattct
      901 tattcttgag taactctttc ctgtaggtca ggttgctttc tcaggtatag tatgaggtcg
      961 ctcttattga ccacacctct accggcagat ccgctaggga taacagggta atatagatcc
     1021 atggtcatag ctgtttcctg a
//
