LOCUS       sequence-164577-         969 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gene Tagging vector HIS/GFP - Plasmid for superfolder GFP (sfGFP) 
            labeling of endogenous proteins with a HIS selection marker, 
            originating from pSIVh (ID:81091).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 969)
  AUTHORS   Wosika V, Durandau E, Varidel C, Aymoz D, Schmitt M, Pelet S
  TITLE     New families of single integration vectors and gene tagging plasmids
            for genetic manipulations in budding yeast.
  JOURNAL   Mol Genet Genomics. 2016 Dec;291(6):2231-2240. Epub 2016 Sep 16.
  PUBMED    27637489
REFERENCE   2  (bases 1 to 969)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Genet 
            Genomics."; date: "2016-12"; volume: "291(6)"; pages: "2231-2240. 
            Epub 2016 Sep 16"
COMMENT     Sequence Label: GTH-g
FEATURES             Location/Qualifiers
     source          1..969
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        91..109
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(138..156)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    complement(157..190)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     terminator      complement(214..411)
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
ORIGIN
        1 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
       61 cgccagcaac gcggccttcg aattaattaa taatacgact cactataggg gacgtccgaa
      121 ttggagctcc aattcgccct atagtgagtc gtattaataa cttcgtatag catacattat
      181 acgaagttat cggcgttagt atcgaatcga cagcagtata gcgaccagca ttcacatacg
      241 attgacgcat gatattactt tctgcgcact taacttcgca tctgggcaga tgatgtcgag
      301 gcgaaaaaaa atataaatca cgctaacatt tgattaaaat agaacaacta caatataaaa
      361 aaactataca aatgacaagt tcttgaaaac aagaatcttt ttattgtcag taatgattac
      421 aacactccct tcgtgcttgg gacttcagaa cttccagtaa gacttgtagc cgcgcgcatg
      481 gcaacagcca gagatttaaa agcgctttca gcacgatgat ggtcattact accatataag
      541 caggtaacat gcaaagtaat tccagcagct accgaaaagg aatatagtaa gtgagggatc
      601 atttcacagg acaattcccc aaccttttca cgctttaatc ccaaatcaat aacagcatag
      661 ggccgtcccg acaaatcaac tacgcttctt gaaagagctt cgtcaagtgg acaataagca
      721 tgtccaaatc ttttaacgcc ggcaaagtta cccatagcct gcttgaatgc aataccaagt
      781 gcaatagcag tatcttctgc tgtgtgatga tcatcaatga ttaaatcacc tcttgagtaa
      841 agtcgtaagc tccagcctgc atgtttagcc agtgcatgat acatgtgatc caagaatcca
      901 attcccgtgt ctacttggat tacttgttct cccttttggt ttgcatgttt ggaagttata
      961 agttcatca
//
