LOCUS       sequence-164578-        4551 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing EIF2A , part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4551)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 4551)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_EIF2A_WT
FEATURES             Location/Qualifiers
     source          1..4551
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2430..2529)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2546..2565)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2547..2565)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2570..2586)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2570..2586)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             3017..3808
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      3901..4489
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4390..4409
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agttggcacc atggcgccgt ccacgccgct cttgacagtc cgaggatcag aaggactgta
      721 catggtgaat ggaccaccac attttacaga aagcacagtg tttccaaggg aatctgggaa
      781 gaattgcaaa gtctgtatct ttagtaagga tgggaccttg tttgcctggg gcaatggaga
      841 aaaagtaaat attatcagtg tcactaacaa gggactactg cactccttcg acctcctgaa
      901 ggcagtttgc cttgaattct cacccaaaaa tactgtcctg gcaacgtggc agccttacac
      961 tacttctaaa gatggcacag ctgggatacc caacctacaa ctttatgatg tgaaaactgg
     1021 gacatgtttg aaatctttca tccagaaaaa aatgcaaaat tggtgtccat cctggtcaga
     1081 agatgaaact ctttgtgccc gcaatgttaa caatgaagtt cacttctttg aaaacaacaa
     1141 ttttaacaca attgcaaata aattgcattt gcaaaaaatt aatgattttg tattatcacc
     1201 tggaccccaa ccatacaagg tggctgtcta tgttccagga agtaaaggtg caccttcatt
     1261 tgttagatta tatcagtacc ccaactttgc tggacctcat gcagctttag ctaataaaag
     1321 tttctttaag gcagataaag ttacaatgct gtggaataaa aaagctactg ctgtgttggt
     1381 aatagctagc acagatgttg acaagacagg agcttcctac tatggagaac aaactctaca
     1441 ctacattgca acaaatggag aaagtgctgt agtgcaatta ccaaaaaatg gccccattta
     1501 tgatgtagtt tggaattcta gttctactga gttttgtgct gtatatggtt ttatgcctgc
     1561 caaagcgaca attttcaact tgaaatgtga tcctgtattt gactttggaa ctggtcctcg
     1621 taatgcagcc tactatagcc ctcatggaca tatattagta ttagctggat ttggaaatct
     1681 gaggggacaa atggaagtgt gggatgtgaa aaactacaaa cttatttcta aaccggtggc
     1741 ttctgattct acatattttg cttggtgccc ggatggtgag catattttaa cagctacatg
     1801 tgctcccagg ttacgggtta ataatggata caaaatttgg cattatactg gctctatctt
     1861 gcacaagtat gatgtgccat caaatgcaga attatggcag gtttcttggc agccattttt
     1921 ggatggaata tttccagcaa aaacaataac ttaccaagca gttccaagtg aagtacccaa
     1981 tgaggaacct aaagttgcaa cagcttatag acccccagct ttaagaaata aaccaatcac
     2041 caattccaaa ttgcatgaag aggaaccacc tcagaatatg aaaccacaat caggaaacga
     2101 taagccatta tcaaaaacag ctcttaaaaa tcaaaggaag catgaagcta agaaagctgc
     2161 aaagcaggaa gcaagaagtg acaagagtcc agatttggca cctactcctg ccccacagag
     2221 cacaccacga aacactgtct ctcagtcaat ttctggggac cctgagatag acaaaaaaat
     2281 caagaaccta aagaagaaac tgaaagcaat cgaacaactg aaagaacaag cagcaactgg
     2341 aaaacagcta gaaaaaaatc agttggagaa aattcagaaa gaaacagccc ttctccagga
     2401 gctggaagat ttggaattgg gtatttaatt gccaactttc ttgtacaaag ttggcattat
     2461 aagaaagcat tgcttatcaa tttgttgcaa cgaacaggtc actatcagtc aaaataaaat
     2521 cattatttgc catccagctg atatccccta tagtgagtcg tattacatgg tcatagctgt
     2581 ttcctggcag ctctggcccg tgtctcaaaa tctctgatgt tacattgcac aagataaaaa
     2641 tatatcatca tgcctcctct agaccagcca ggacagaaat gcctcgactt cgctgctacc
     2701 caaggttgcc gggtgacgca caccgtggaa acggatgaag gcacgaaccc agtggacata
     2761 agcctgttcg gttcgtaagc tgtaatgcaa gtagcgtatg cgctcacgca actggtccag
     2821 aaccttgacc gaacgcagcg gtggtaacgg cgcagtggcg gttttcatgg cttgttatga
     2881 ctgttttttt ggggtacagt ctatgcctcg ggcatccaag cagcaagcgc gttacgccgt
     2941 gggtcgatgt ttgatgttat ggagcagcaa cgatgttacg cagcagggca gtcgccctaa
     3001 aacaaagtta aacattatga gggaagcggt gatcgccgaa gtatcgactc aactatcaga
     3061 ggtagttggc gtcatcgagc gccatctcga accgacgttg ctggccgtac atttgtacgg
     3121 ctccgcagtg gatggcggcc tgaagccaca cagtgatatt gatttgctgg ttacggtgac
     3181 cgtaaggctt gatgaaacaa cgcggcgagc tttgatcaac gaccttttgg aaacttcggc
     3241 ttcccctgga gagagcgaga ttctccgcgc tgtagaagtc accattgttg tgcacgacga
     3301 catcattccg tggcgttatc cagctaagcg cgaactgcaa tttggagaat ggcagcgcaa
     3361 tgacattctt gcaggtatct tcgagccagc cacgatcgac attgatctgg ctatcttgct
     3421 gacaaaagca agagaacata gcgttgcctt ggtaggtcca gcggcggagg aactctttga
     3481 tccggttcct gaacaggatc tatttgaggc gctaaatgaa accttaacgc tatggaactc
     3541 gccgcccgac tgggctggcg atgagcgaaa tgtagtgctt acgttgtccc gcatttggta
     3601 cagcgcagta accggcaaaa tcgcgccgaa ggatgtcgct gccgactggg caatggagcg
     3661 cctgccggcc cagtatcagc ccgtcatact tgaagctaga caggcttatc ttggacaaga
     3721 agaagatcgc ttggcctcgc gcgcagatca gttggaagaa tttgtccact acgtgaaagg
     3781 cgagatcacc aaggtagtcg gcaaataacc ctcgagccac ccatgaccaa aatcccttaa
     3841 cgtgagttac gcgtcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
     3901 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
     3961 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
     4021 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
     4081 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
     4141 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
     4201 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
     4261 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
     4321 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
     4381 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
     4441 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
     4501 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt t
//
