LOCUS       sequence-164580-         924 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gene Tagging vector HIS/YFP - Plasmid for monomeric Citrine 
            (mCitrine) labeling of endogenous proteins with a HIS selection 
            marker, originating from pSIVh (ID:81091).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 924)
  AUTHORS   Wosika V, Durandau E, Varidel C, Aymoz D, Schmitt M, Pelet S
  TITLE     New families of single integration vectors and gene tagging plasmids
            for genetic manipulations in budding yeast.
  JOURNAL   Mol Genet Genomics. 2016 Dec;291(6):2231-2240. Epub 2016 Sep 16.
  PUBMED    27637489
REFERENCE   2  (bases 1 to 924)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Genet 
            Genomics."; date: "2016-12"; volume: "291(6)"; pages: "2231-2240. 
            Epub 2016 Sep 16"
COMMENT     Sequence Label: GTH-y
FEATURES             Location/Qualifiers
     source          1..924
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        89..107
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(136..154)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    complement(155..188)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     terminator      complement(212..409)
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
ORIGIN
        1 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
       61 ccagcaacgc ggccttcgaa ttaattaata atacgactca ctatagggga cgtccgaatt
      121 ggagctccaa ttcgccctat agtgagtcgt attaataact tcgtatagca tacattatac
      181 gaagttatcg gcgttagtat cgaatcgaca gcagtatagc gaccagcatt cacatacgat
      241 tgacgcatga tattactttc tgcgcactta acttcgcatc tgggcagatg atgtcgaggc
      301 gaaaaaaaat ataaatcacg ctaacatttg attaaaatag aacaactaca atataaaaaa
      361 actatacaaa tgacaagttc ttgaaaacaa gaatcttttt attgtcagta atgattacaa
      421 cactcccttc gtgcttggga cttcagaact tccagtaaga cttgtagccg cgcgcatggc
      481 aacagccaga gatttaaaag cgctttcagc acgatgatgg tcattactac catataagca
      541 ggtaacatgc aaagtaattc cagcagctac cgaaaaggaa tatagtaagt gagggatcat
      601 ttcacaggac aattccccaa ccttttcacg ctttaatccc aaatcaataa cagcataggg
      661 ccgtcccgac aaatcaacta cgcttcttga aagagcttcg tcaagtggac aataagcatg
      721 tccaaatctt ttaacgccgg caaagttacc catagcctgc ttgaatgcaa taccaagtgc
      781 aatagcagta tcttctgctg tgtgatgatc atcaatgatt aaatcacctc ttgagtaaag
      841 tcgtaagctc cagcctgcat gtttagccag tgcatgatac atgtgatcca agaatccaat
      901 tcccgtgtct acttggatta cttg
//
