LOCUS       sequence-164583-         938 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gene Tagging vector HIS/RFP - Plasmid for monomeric Cherry (mCherry)
            labeling of endogenous proteins with a HIS selection marker, 
            originating from pSIVh (ID:81091).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 938)
  AUTHORS   Wosika V, Durandau E, Varidel C, Aymoz D, Schmitt M, Pelet S
  TITLE     New families of single integration vectors and gene tagging plasmids
            for genetic manipulations in budding yeast.
  JOURNAL   Mol Genet Genomics. 2016 Dec;291(6):2231-2240. Epub 2016 Sep 16.
  PUBMED    27637489
REFERENCE   2  (bases 1 to 938)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Genet 
            Genomics."; date: "2016-12"; volume: "291(6)"; pages: "2231-2240. 
            Epub 2016 Sep 16"
COMMENT     Sequence Label: GTH-r
FEATURES             Location/Qualifiers
     source          1..938
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        90..108
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(137..155)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    complement(156..189)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     terminator      complement(213..410)
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
ORIGIN
        1 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
       61 gccagcaacg cggccttcga attaattaat aatacgactc actatagggg acgtccgaat
      121 tggagctcca attcgcccta tagtgagtcg tattaataac ttcgtatagc atacattata
      181 cgaagttatc ggcgttagta tcgaatcgac agcagtatag cgaccagcat tcacatacga
      241 ttgacgcatg atattacttt ctgcgcactt aacttcgcat ctgggcagat gatgtcgagg
      301 cgaaaaaaaa tataaatcac gctaacattt gattaaaata gaacaactac aatataaaaa
      361 aactatacaa atgacaagtt cttgaaaaca agaatctttt tattgtcagt aatgattaca
      421 acactccctt cgtgcttggg acttcagaac ttccagtaag acttgtagcc gcgcgcatgg
      481 caacagccag agatttaaaa gcgctttcag cacgatgatg gtcattacta ccatataagc
      541 aggtaacatg caaagtaatt ccagcagcta ccgaaaagga atatagtaag tgagggatca
      601 tttcacagga caattcccca accttttcac gctttaatcc caaatcaata acagcatagg
      661 gccgtcccga caaatcaact acgcttcttg aaagagcttc gtcaagtgga caataagcat
      721 gtccaaatct tttaacgccg gcaaagttac ccatagcctg cttgaatgca ataccaagtg
      781 caatagcagt atcttctgct gtgtgatgat catcaatgat taaatcacct cttgagtaaa
      841 gtcgtaagct ccagcctgca tgtttagcca gtgcatgata catgtgatcc aagaatccaa
      901 ttcccgtgtc tacttggatt acttgttctc ccttttgg
//
