LOCUS       sequence-164588-        4183 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gene Tagging vector TRP/CFP - Plasmid for yeast enhanced monomeric 
            CFP (yemCFP) labeling of endogenous proteins with a TRP selection 
            marker, originating from pSIVt (ID:81092).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4183)
  AUTHORS   Wosika V, Durandau E, Varidel C, Aymoz D, Schmitt M, Pelet S
  TITLE     New families of single integration vectors and gene tagging plasmids
            for genetic manipulations in budding yeast.
  JOURNAL   Mol Genet Genomics. 2016 Dec;291(6):2231-2240. Epub 2016 Sep 16.
  PUBMED    27637489
REFERENCE   2  (bases 1 to 4183)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Genet 
            Genomics."; date: "2016-12"; volume: "291(6)"; pages: "2231-2240. 
            Epub 2016 Sep 16"
COMMENT     Sequence Label: GTT-c
FEATURES             Location/Qualifiers
     source          1..4183
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(69..132)
                     /gene="bla"
                     /label=AmpR promoter
     promoter        159..177
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     CDS             209..925
                     /codon_start=1
                     /product="yeast-enhanced green fluorescent protein"
                     /label=yeGFP
                     /note="codons optimized for translation in S. cerevisiae 
                     and C. albicans"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYIYNSHNVYITADKQKNGIKA
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSRLSKDPNEKRDHMVLLE
                     FVTAAGITHGIDELYK"
     terminator      969..1156
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(1024..1048)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     promoter        1231..1574
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     protein_bind    2500..2533
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        2534..2552
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(2581..2599)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      complement(2632..3220)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2712..2731)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3391..4182,4183,1..68))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFFHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4014..4033
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
       61 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
      121 agcggataca tatttgaagt atcgttcgaa ttaattaaaa ttaaccctca ctaaaggggc
      181 atgcgcggcc gctctagaac taattaacat gtctaaaggt gaagaattat tcactggtgt
      241 tgtcccaatt ttggttgaat tagatggtga tgttaatggt cacaaatttt ctgtctccgg
      301 tgaaggtgaa ggtgatgcta cttacggtaa attgacctta aaatttattt gtactactgg
      361 taaattgcca gttccatggc caaccttagt cactacttta acttggggtg ttcaatgttt
      421 ttctagatac ccagatcata tgaaacaaca tgactttttc aagtctgcca tgccagaagg
      481 ttatgttcaa gaaagaacta tttttttcaa agatgacggt aactacaaga ccagagctga
      541 agtcaagttt gaaggtgata ccttagttaa tagaatcgaa ttaaaaggta ttgattttaa
      601 agaagatggt aacattttag gtcacaaatt ggaatacatt tataactctc acaatgttta
      661 catcactgct gacaaacaaa agaatggtat caaagctaac ttcaaaatta gacacaacat
      721 tgaagatggt tctgttcaat tagctgacca ttatcaacaa aatactccaa ttggtgatgg
      781 tccagtcttg ttaccagaca accattactt atccactcaa tctagattat ccaaagatcc
      841 aaacgaaaag agagaccaca tggtcttgtt agaatttgtt actgctgctg gtattaccca
      901 tggtattgat gaattgtaca aataaggatg ctagcatttt aactcgaggg cgcgccactt
      961 ctaaataagc gaatttctta tgatttatga tttttattat taaataagtt ataaaaaaaa
     1021 taagtgtata caaattttaa agtgactctt aggttttaaa acgaaaattc ttattcttga
     1081 gtaactcttt cctgtaggtc aggttgcttt ctcaggtata gtatgaggtc gctcttattg
     1141 accacacctc taccggcaga tccgctaggg ataacagggt aatatagatc tgtttagctt
     1201 gcctcgtccc cgccgggtca cccggcgagc gacatggagg cccagaatac cctccttgac
     1261 agtcttgacg tgcgcagctc aggggcatga tgtgactgtc gcccgtacat ttagcccata
     1321 catccccatg tataatcatt tgcatccata cattttgatg gccgcacggc gcgaagcaaa
     1381 aattacggct cctcgcctca gacctgcgag cagggaaacg ctcccctcac agacgcgttg
     1441 aattgtcccc acgccgcgcc cctgtagaga aatataaaag gttaggattt gccactgagg
     1501 ttcttctttc atatacttcc ttttaaaatc ttgctaggat acagttctca catcacatcc
     1561 gaacataaac aaccatgagt gtcataaact ttaccggatc gagtggacct ctcgtcaagg
     1621 tatgcgggct ccagtccact gaagcagcgg agtgtgccct agactctgat gccgatctct
     1681 tgggcattat ctgcgtcccg aataggaaac gcaccattga tcctgtaatc gcgcgcaaga
     1741 tttcttcgct cgtaaaggcc tacaagaact cttcaggaac gccgaagtat ttagtcggtg
     1801 tttttcgtaa tcagcctaaa gaggacgtat tggcactcgt aaacgactac ggcatagata
     1861 tagtgcagtt acatggggac gagagctggc aggaatatca agagttcctg ggcttgcctg
     1921 tcattaaacg actggttttt ccgaaagatt gtaatatcct tttgtcagca gcgtcgcaga
     1981 aaccgcactc attcataccg ctcttcgact cggaagcagg cggtacaggg gaactactgg
     2041 actggaatag tataagtgac tgggtcggac ggcaagagag tccggagtcc ttacatttta
     2101 tgctggccgg gggtttaacc cccgaaaacg tgggagatgc cctccgatta aatggggtta
     2161 tcggtgtcga tgtatcaggt ggggtggaga cgaatggcgt gaaggactca aataaaatcg
     2221 ctaattttgt gaaaaacgct aagaaatgag aatattgata aatttaaatt ttggaatgag
     2281 ggttgtcctt tcctttacaa tagaggcatt tgagtgagta ggttttatca ccttcttgct
     2341 cttattaaat cgcagttgtc tttccggtat gtgcttgact gaggaacaat attttattca
     2401 tgatacattt ccatgctacg catatcgtca tccacatgga aggtaatttt cttctgtggc
     2461 ctttcatata gttgtgaatg gtagaacaat cttcatataa taacttcgta taatgtatgc
     2521 tatacgaagt tattaatacg actcactata gggcgaattg gagctccaat tcggacgtcc
     2581 cctatagtga gtcgtattat taattaattc gaaggccgcg ttgctggcgt ttttccatag
     2641 gctcggcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
     2701 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
     2761 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
     2821 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
     2881 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
     2941 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
     3001 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
     3061 ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
     3121 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
     3181 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
     3241 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
     3301 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta
     3361 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat
     3421 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctgcccgtcg tgtagataac
     3481 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg
     3541 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag
     3601 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt
     3661 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt
     3721 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt
     3781 tacatgatcc cccatgttgt gaaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt
     3841 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct
     3901 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt
     3961 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac
     4021 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa
     4081 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa
     4141 ctgatcttca gcatctttta ctttcaccag cgtttctggg tga
//
