LOCUS       sequence-164592-        6555 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing ERBB2 , part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6555)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 6555)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_ERBB2_WT
FEATURES             Location/Qualifiers
     source          1..6555
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(4434..4533)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(4550..4569)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4551..4569)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4574..4590)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4574..4590)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             5021..5812
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      5905..6493
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6394..6413
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agttggcatg gagctggcgg ccttgtgccg ctgggggctc ctcctcgccc tcttgccccc
      721 cggagccgcg agcacccaag tgtgcaccgg cacagacatg aagctgcggc tccctgccag
      781 tcccgagacc cacctggaca tgctccgcca cctctaccag ggctgccagg tggtgcaggg
      841 aaacctggaa ctcacctacc tgcccaccaa tgccagcctg tccttcctgc aggatatcca
      901 ggaggtgcag ggctacgtgc tcatcgctca caaccaagtg aggcaggtcc cactgcagag
      961 gctgcggatt gtgcgaggca cccagctctt tgaggacaac tatgccctgg ccgtgctaga
     1021 caatggagac ccgctgaaca ataccacccc tgtcacaggg gcctccccag gaggcctgcg
     1081 ggagctgcag cttcgaagcc tcacagagat cttgaaagga ggggtcttga tccagcggaa
     1141 cccccagctc tgctaccagg acacgatttt gtggaaggac atcttccaca agaacaacca
     1201 gctggctctc acactgatag acaccaaccg ctctcgggcc tgccacccct gttctccgat
     1261 gtgtaagggc tcccgctgct ggggagagag ttctgaggat tgtcagagcc tgacgcgcac
     1321 tgtctgtgcc ggtggctgtg cccgctgcaa ggggccactg cccactgact gctgccatga
     1381 gcagtgtgct gccggctgca cgggccccaa gcactctgac tgcctggcct gcctccactt
     1441 caaccacagt ggcatctgtg agctgcactg cccagccctg gtcacctaca acacagacac
     1501 gtttgagtcc atgcccaatc ccgagggccg gtatacattc ggcgccagct gtgtgactgc
     1561 ctgtccctac aactaccttt ctacggacgt gggatcctgc accctcgtct gccccctgca
     1621 caaccaagag gtgacagcag aggatggaac acagcggtgt gagaagtgca gcaagccctg
     1681 tgcccgagtg tgctatggtc tgggcatgga gcacttgcga gaggtgaggg cagttaccag
     1741 tgccaatatc caggagtttg ctggctgcaa gaagatcttt gggagcctgg catttctgcc
     1801 ggagagcttt gatggggacc cagcctccaa cactgccccg ctccagccag agcagctcca
     1861 agtgtttgag actctggaag agatcacagg ttacctatac atctcagcat ggccggacag
     1921 cctgcctgac ctcagcgtct tccagaacct gcaagtaatc cggggacgaa ttctgcacaa
     1981 tggcgcctac tcgctgaccc tgcaagggct gggcatcagc tggctggggc tgcgctcact
     2041 gagggaactg ggcagtggac tggccctcat ccaccataac acccacctct gcttcgtgca
     2101 cacggtgccc tgggaccagc tctttcggaa cccgcaccaa gctctgctcc acactgccaa
     2161 ccggccagag gacgagtgtg tgggcgaggg cctggcctgc caccagctgt gcgcccgagg
     2221 gcactgctgg ggtccagggc ccacccagtg tgtcaactgc agccagttcc ttcggggcca
     2281 ggagtgcgtg gaggaatgcc gagtactgca ggggctcccc agggagtatg tgaatgccag
     2341 gcactgtttg ccgtgccacc ctgagtgtca gccccagaat ggctcagtga cctgttttgg
     2401 accggaggct gaccagtgtg tggcctgtgc ccactataag gaccctccct tctgcgtggc
     2461 ccgctgcccc agcggtgtga aacctgacct ctcctacatg cccatctgga agtttccaga
     2521 tgaggagggc gcatgccagc cttgccccat caactgcacc cactcctgtg tggacctgga
     2581 tgacaagggc tgccccgccg agcagagagc cagccctctg acgtccatca tctctgcggt
     2641 ggttggcatt ctgctggtcg tggtcttggg ggtggtcttt gggatcctca tcaagcgacg
     2701 gcagcagaag atccggaagt acacgatgcg gagactgctg caggaaacgg agctggtgga
     2761 gccgctgaca cctagcggag cgatgcccaa ccaggcgcag atgcggatcc tgaaagagac
     2821 ggagctgagg aaggtgaagg tgcttggatc tggcgctttt ggcacagtct acaagggcat
     2881 ctggatccct gatggggaga atgtgaaaat tccagtggcc atcaaagtgt tgagggaaaa
     2941 cacatccccc aaagccaaca aagaaatctt agacgaagca tacgtgatgg ctggtgtggg
     3001 ctccccatat gtctcccgcc ttctgggcat ctgcctgaca tccacggtgc agctggtgac
     3061 acagcttatg ccctatggct gcctcttaga ccatgtccgg gaaaaccgcg gacgcctggg
     3121 ctcccaggac ctgctgaact ggtgtatgca gattgccaag gggatgagct acctggagga
     3181 tgtgcggctc gtacacaggg acttggccgc tcggaacgtg ctggtcaaga gtcccaacca
     3241 tgtcaaaatt acagacttcg ggctggctcg gctgctggac attgacgaga cagagtacca
     3301 tgcagatggg ggcaaggtgc ccatcaagtg gatggcgctg gagtccattc tccgccggcg
     3361 gttcacccac cagagtgatg tgtggagtta tggtgtgact gtgtgggagc tgatgacttt
     3421 tggggccaaa ccttacgatg ggatcccagc ccgggagatc cctgacctgc tggaaaaggg
     3481 ggagcggctg ccccagcccc ccatctgcac cattgatgtc tacatgatca tggtcaaatg
     3541 ttggatgatt gactctgaat gtcggccaag attccgggag ttggtgtctg aattctcccg
     3601 catggccagg gacccccagc gctttgtggt catccagaat gaggacttgg gcccagccag
     3661 tcccttggac agcaccttct accgctcact gctggaggac gatgacatgg gggacctggt
     3721 ggatgctgag gagtatctgg taccccagca gggcttcttc tgtccagacc ctgccccggg
     3781 cgctgggggc atggtccacc acaggcaccg cagctcatct accaggagtg gcggtgggga
     3841 cctgacacta gggctggagc cctctgaaga ggaggccccc aggtctccac tggcaccctc
     3901 cgaaggggct ggctccgatg tatttgatgg tgacctggga atgggggcag ccaaggggct
     3961 gcaaagcctc cccacacatg accccagccc tctacagcgg tacagtgagg accccacagt
     4021 acccctgccc tctgagactg atggctacgt tgcccccctg acctgcagcc cccagcctga
     4081 atatgtgaac cagccagatg ttcggcccca gcccccttcg ccccgagagg gccctctgcc
     4141 tgctgcccga cctgctggtg ccactctgga aaggcccaag actctctccc cagggaagaa
     4201 tggggtcgtc aaagacgttt ttgcctttgg gggtgccgtg gagaaccccg agtacttgac
     4261 accccaggga ggagctgccc ctcagcccca ccctcctcct gccttcagcc cagccttcga
     4321 caacctctat tactgggacc aggacccacc agagcggggg gctccaccca gcaccttcaa
     4381 agggacacct acggcagaga acccagagta cctgggtctg gacgtgccag tgtgaccaac
     4441 tttcttgtac aaagttggca ttataagaaa gcattgctta tcaatttgtt gcaacgaaca
     4501 ggtcactatc agtcaaaata aaatcattat ttgccatcca gctgatatcc cctatagtga
     4561 gtcgtattac atggtcatag ctgtttcctg gcagctctgg cccgtgtctc aaaatctctg
     4621 atgttacatt gcacaagata aaaatatatc atcatgcctc ctctagacca gccaggacag
     4681 aaatgcctcg acttcgctgc tacccaaggt tgccgggtga cgcacaccgt ggaaacggat
     4741 gaaggcacga acccagtgga cataagcctg ttcggttcgt aagctgtaat gcaagtagcg
     4801 tatgcgctca cgcaactggt ccagaacctt gaccgaacgc agcggtggta acggcgcagt
     4861 ggcggttttc atggcttgtt atgactgttt ttttggggta cagtctatgc ctcgggcatc
     4921 caagcagcaa gcgcgttacg ccgtgggtcg atgtttgatg ttatggagca gcaacgatgt
     4981 tacgcagcag ggcagtcgcc ctaaaacaaa gttaaacatt atgagggaag cggtgatcgc
     5041 cgaagtatcg actcaactat cagaggtagt tggcgtcatc gagcgccatc tcgaaccgac
     5101 gttgctggcc gtacatttgt acggctccgc agtggatggc ggcctgaagc cacacagtga
     5161 tattgatttg ctggttacgg tgaccgtaag gcttgatgaa acaacgcggc gagctttgat
     5221 caacgacctt ttggaaactt cggcttcccc tggagagagc gagattctcc gcgctgtaga
     5281 agtcaccatt gttgtgcacg acgacatcat tccgtggcgt tatccagcta agcgcgaact
     5341 gcaatttgga gaatggcagc gcaatgacat tcttgcaggt atcttcgagc cagccacgat
     5401 cgacattgat ctggctatct tgctgacaaa agcaagagaa catagcgttg ccttggtagg
     5461 tccagcggcg gaggaactct ttgatccggt tcctgaacag gatctatttg aggcgctaaa
     5521 tgaaacctta acgctatgga actcgccgcc cgactgggct ggcgatgagc gaaatgtagt
     5581 gcttacgttg tcccgcattt ggtacagcgc agtaaccggc aaaatcgcgc cgaaggatgt
     5641 cgctgccgac tgggcaatgg agcgcctgcc ggcccagtat cagcccgtca tacttgaagc
     5701 tagacaggct tatcttggac aagaagaaga tcgcttggcc tcgcgcgcag atcagttgga
     5761 agaatttgtc cactacgtga aaggcgagat caccaaggta gtcggcaaat aaccctcgag
     5821 ccacccatga ccaaaatccc ttaacgtgag ttacgcgtcg ttccactgag cgtcagaccc
     5881 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
     5941 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
     6001 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt
     6061 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
     6121 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
     6181 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
     6241 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
     6301 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
     6361 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
     6421 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
     6481 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
     6541 ttttgctcac atgtt
//
