LOCUS       sequence-164594-         994 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gene Tagging vector TRP/YFP - Plasmid for monomeric Citrine 
            (mCitrine) labeling of endogenous proteins with a TRP selection 
            marker, originating from pSIVt (ID:81092).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 994)
  AUTHORS   Wosika V, Durandau E, Varidel C, Aymoz D, Schmitt M, Pelet S
  TITLE     New families of single integration vectors and gene tagging plasmids
            for genetic manipulations in budding yeast.
  JOURNAL   Mol Genet Genomics. 2016 Dec;291(6):2231-2240. Epub 2016 Sep 16.
  PUBMED    27637489
REFERENCE   2  (bases 1 to 994)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Genet 
            Genomics."; date: "2016-12"; volume: "291(6)"; pages: "2231-2240. 
            Epub 2016 Sep 16"
COMMENT     Sequence Label: GTT-y
FEATURES             Location/Qualifiers
     source          1..994
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        2..20
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     CDS             52..762
                     /codon_start=1
                     /product="yeast-enhanced green fluorescent protein"
                     /label=yeGFP
                     /note="codons optimized for translation in S. cerevisiae 
                     and C. albicans"
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF
                     ICTTGKLPVPWPTLVTTLGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVN
                     FKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLKEF
                     VTAAGITHGMDELYK"
     terminator      807..994
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(862..886)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
ORIGIN
        1 aaattaaccc tcactaaagg ggcatgcgcg gccgctctag aactatctgc ttctaaaggt
       61 gaagaattat tcactggtgt tgtcccaatt ttggttgaat tagatggtga tgttaatggt
      121 cacaaatttt ctgtctccgg tgaaggtgaa ggtgatgcta cttacggtaa attgacctta
      181 aaatttattt gtactactgg taaattgcca gttccatggc caaccttagt cactacttta
      241 ggttatggtt tgatgtgttt tgctagatac ccagatcata tgaaacaaca tgactttttc
      301 aagtctgcca tgccagaagg ttatgttcaa gaaagaacta tttttttcaa agatgacggt
      361 aactacaaga ccagagctga agtcaagttt gaaggtgata ccttagttaa tagaatcgaa
      421 ttaaaaggta ttgattttaa agaagatggt aacattttag gtcacaaatt ggaatacaac
      481 tataactctc acaatgttta catcatggct gacaaacaaa agaatggtat caaagttaac
      541 ttcaaaatta gacacaacat tgaagatggt tctgttcaat tagctgacca ttatcaacaa
      601 aatactccaa ttggtgatgg tccagtcttg ttaccagaca accattactt atcctatcaa
      661 tctaaattat ccaaagatcc aaacgaaaag agagaccaca tggtcttgaa agaatttgtt
      721 actgctgctg gtattaccca tggtatggat gaattgtaca aaggaatgct agcattttaa
      781 ctcgagggcg cgccacttct aaataagcga atttcttatg atttatgatt tttattatta
      841 aataagttat aaaaaaaata agtgtataca aattttaaag tgactcttag gttttaaaac
      901 gaaaattctt attcttgagt aactctttcc tgtaggtcag gttgctttct caggtatagt
      961 atgaggtcgc tcttattgac cacacctcta ccgg
//
