LOCUS       sequence-164597-        5727 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing ERN1 , part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5727)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 5727)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_ERN1_WT
FEATURES             Location/Qualifiers
     source          1..5727
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(3606..3705)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(3722..3741)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3723..3741)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3746..3762)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3746..3762)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             4193..4984
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      5077..5665
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5566..5585
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agttggcacc atgccggccc ggcggctgct gctgctgctg acgctgctgc tgcccggcct
      721 cgggattttt ggaagtacca gcacagtgac gcttcctgaa accttgttgt ttgtgtcaac
      781 gctggatgga agtttgcatg ctgtcagcaa gaggacaggc tcaatcaaat ggactttaaa
      841 agaagatcca gtcctgcagg tcccaacaca tgtggaagag cctgcctttc tcccagatcc
      901 taatgatggc agcctgtata cgcttggaag caagaataat gaaggcctga cgaaacttcc
      961 ttttaccatc ccagaattgg tgcaggcatc cccatgccga agttcagatg gaatcctcta
     1021 catgggtaaa aagcaggaca tctggtatgt tattgacctc ctgaccggag agaagcagca
     1081 gactttgtca tcggcctttg cagatagtct ctgcccatca acctctcttc tgtatcttgg
     1141 gcgaacagaa tacaccatca ccatgtacga caccaaaacc cgagagctcc ggtggaatgc
     1201 cacctacttt gactatgcgg cctcactgcc tgaggacgac gtggactaca agatgtccca
     1261 ctttgtgtcc aatggtgatg ggctggtggt gactgtggac agtgaatctg gggacgtcct
     1321 gtggatccaa aactacgcct cccctgtggt ggccttttat gtctggcagc gggagggtct
     1381 gaggaaggtg atgcacatca atgtcgctgt ggagaccctg cgctatctga ccttcatgtc
     1441 tggggaggtg gggcgcatca caaagtggaa gtacccgttc cccaaggaga cagaggccaa
     1501 gagcaagctg acgcccactc tgtatgttgg gaaatactct accagcctct atgcctctcc
     1561 ctcaatggta cacgaggggg ttgctgtcgt gccccgcggc agcacacttc ctttgctgga
     1621 agggccccag actgatggcg tcaccatcgg ggacaagggg gagtgtgtga tcacgcccag
     1681 cacggacgtc aagtttgatc ccggactcaa aagcaagaac aagctcaact acttgaggaa
     1741 ttactggctt ctgataggac accatgaaac cccactgtct gcgtctacca agatgctgga
     1801 gagatttccc aacaatctac ccaaacatcg ggaaaatgtg attcctgctg attcagagaa
     1861 aaagagcttt gaggaagtta tcaacctggt tgaccagact tcagaaaacg cacctaccac
     1921 cgtgtctcgg gatgtggagg agaagcccgc ccatgcccct gcccggcccg aggcccccgt
     1981 ggactccatg cttaaggaca tggctaccat catcctgagc accttcctgc tgattggctg
     2041 ggtggccttc atcatcacct atcccctgag catgcatcag cagcagcagc tccagcacca
     2101 gcagttccag aaggaactgg agaagatcca gctcctgcag cagcagcagc agcagctgcc
     2161 cttccaccca cctggagaca cggctcagga cggcgagctc ctggacacgt ctggcccgta
     2221 ctcagagagc tcgggcacca gcagccccag cacgtccccc agggcctcca accactcgct
     2281 ctgctccggc agctctgcct ccaaggctgg cagcagcccc tccctggaac aagacgatgg
     2341 agatgaggaa accagcgtgg tgatagttgg gaaaatttcc ttctgtccca aggatgtcct
     2401 gggccatgga gctgagggca caattgtgta ccggggcatg tttgacaacc gcgacgtggc
     2461 cgtgaagagg atcctccccg agtgttttag cttcgcagac cgtgaggtcc agctgttgcg
     2521 agaatcggat gagcacccga acgtgatccg ctacttctgc acggagaagg accggcaatt
     2581 ccagtacatt gccatcgagc tgtgtgcagc caccctgcaa gagtatgtgg agcagaagga
     2641 ctttgcgcat ctcggcctgg agcccatcac cttgctgcag cagaccacct cgggcctggc
     2701 ccacctccac tccctcaaca tcgttcacag agacctaaag ccacacaaca tcctcatatc
     2761 catgcccaat gcacacggca agatcaaggc catgatctcc gactttggcc tctgcaagaa
     2821 gctggcagtg ggcagacaca gtttcagccg ccgatctggg gtgcctggca cagaaggctg
     2881 gatcgctcca gagatgctga gcgaagactg taaggagaac cctacctaca cggtggacat
     2941 cttttctgca ggctgcgtct tttactacgt aatctctgag ggcagccacc cttttggcaa
     3001 gtccctgcag cggcaggcca acatcctcct gggtgcctgc agccttgact gcttgcaccc
     3061 agagaagcac gaagacgtca ttgcacgtga attgatagag aagatgattg cgatggatcc
     3121 tcagaaacgc ccctcagcga agcacgtgct caaacacccg ttcttctgga gcctagagaa
     3181 gcagctccag ttcttccagg acgtgagcga cagaatagaa aaggaatccc tggatggccc
     3241 gatcgtgaag cagttagaga gaggcgggag agccgtggtg aagatggact ggcgggagaa
     3301 catcactgtc cccctccaga cagacctgcg taaattcagg acctataaag gtggttctgt
     3361 cagagatctc ctccgagcca tgagaaataa gaagcaccac taccgggagc tgcctgcaga
     3421 ggtgcgggag acgctggggt ccctccccga cgacttcgtg tgctacttca cgtctcgctt
     3481 cccccacctc ctcgcacaca cctaccgggc catggagctg tgcagccacg agagactctt
     3541 ccagccctac tacttccacg agcccccaga gccccagccc ccagtgactc cagacgccct
     3601 ctgattgcca actttcttgt acaaagttgg cattataaga aagcattgct tatcaatttg
     3661 ttgcaacgaa caggtcacta tcagtcaaaa taaaatcatt atttgccatc cagctgatat
     3721 cccctatagt gagtcgtatt acatggtcat agctgtttcc tggcagctct ggcccgtgtc
     3781 tcaaaatctc tgatgttaca ttgcacaaga taaaaatata tcatcatgcc tcctctagac
     3841 cagccaggac agaaatgcct cgacttcgct gctacccaag gttgccgggt gacgcacacc
     3901 gtggaaacgg atgaaggcac gaacccagtg gacataagcc tgttcggttc gtaagctgta
     3961 atgcaagtag cgtatgcgct cacgcaactg gtccagaacc ttgaccgaac gcagcggtgg
     4021 taacggcgca gtggcggttt tcatggcttg ttatgactgt ttttttgggg tacagtctat
     4081 gcctcgggca tccaagcagc aagcgcgtta cgccgtgggt cgatgtttga tgttatggag
     4141 cagcaacgat gttacgcagc agggcagtcg ccctaaaaca aagttaaaca ttatgaggga
     4201 agcggtgatc gccgaagtat cgactcaact atcagaggta gttggcgtca tcgagcgcca
     4261 tctcgaaccg acgttgctgg ccgtacattt gtacggctcc gcagtggatg gcggcctgaa
     4321 gccacacagt gatattgatt tgctggttac ggtgaccgta aggcttgatg aaacaacgcg
     4381 gcgagctttg atcaacgacc ttttggaaac ttcggcttcc cctggagaga gcgagattct
     4441 ccgcgctgta gaagtcacca ttgttgtgca cgacgacatc attccgtggc gttatccagc
     4501 taagcgcgaa ctgcaatttg gagaatggca gcgcaatgac attcttgcag gtatcttcga
     4561 gccagccacg atcgacattg atctggctat cttgctgaca aaagcaagag aacatagcgt
     4621 tgccttggta ggtccagcgg cggaggaact ctttgatccg gttcctgaac aggatctatt
     4681 tgaggcgcta aatgaaacct taacgctatg gaactcgccg cccgactggg ctggcgatga
     4741 gcgaaatgta gtgcttacgt tgtcccgcat ttggtacagc gcagtaaccg gcaaaatcgc
     4801 gccgaaggat gtcgctgccg actgggcaat ggagcgcctg ccggcccagt atcagcccgt
     4861 catacttgaa gctagacagg cttatcttgg acaagaagaa gatcgcttgg cctcgcgcgc
     4921 agatcagttg gaagaatttg tccactacgt gaaaggcgag atcaccaagg tagtcggcaa
     4981 ataaccctcg agccacccat gaccaaaatc ccttaacgtg agttacgcgt cgttccactg
     5041 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
     5101 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
     5161 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
     5221 tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
     5281 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
     5341 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
     5401 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
     5461 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
     5521 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
     5581 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
     5641 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
     5701 cttttgctgg ccttttgctc acatgtt
//
