LOCUS       sequence-164599-        4182 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing ERRFI1 , part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4182)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 4182)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_ERRFI1_WT
FEATURES             Location/Qualifiers
     source          1..4182
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2061..2160)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2177..2196)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2178..2196)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2201..2217)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2201..2217)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             2648..3439
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      3532..4120
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4021..4040
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agttggcacc atgtcaatag caggagttgc tgctcaggag atcagagtcc cattaaaaac
      721 tggatttcta cataatggcc gagccatggg gaatatgagg aagacctact ggagcagtcg
      781 cagtgagttt aaaaacaact ttttaaatat tgacccgata accatggcct acagtctgaa
      841 ctcttctgct caggagcgcc taataccact tgggcatgct tccaaatctg ctccgatgaa
      901 tggccactgc tttgcagaaa atggtccatc tcaaaagtcc agcttgcccc ctcttcttat
      961 tcccccaagt gaaaacttgg gaccacatga agaggatcaa gttgtatgtg gttttaagaa
     1021 actcacagtg aatggggttt gtgcttccac ccctccactg acacccataa aaaactcccc
     1081 ttcccttttc ccctgtgccc ctctttgtga acggggttct aggcctcttc caccgttgcc
     1141 aatctctgaa gccctctctc tggatgacac agactgtgag gtggaattcc taactagctc
     1201 agatacagac ttccttttag aagactctac actttctgat ttcaaatatg atgttcctgg
     1261 caggcgaagc ttccgtgggt gtggacaaat caactatgca tattttgata ccccagctgt
     1321 ttctgcagca gatctcagct atgtgtctga ccaaaatgga ggtgtcccag atccaaatcc
     1381 tcctccacct cagacccacc gaagattaag aaggtctcat tcgggaccag ctggctcctt
     1441 taacaagcca gccataagga tatccaactg ttgtatacac agagcttctc ctaactccga
     1501 tgaagacaaa cctgaggttc cccccagagt tcccatacct cctagaccag taaagccaga
     1561 ttatagaaga tggtcagcag aagttacttc gagcacctat agtgatgaag acaggcctcc
     1621 caaagtaccg ccaagagaac ctttgtcacc gagtaactcg cgcacaccga gtcccaaaag
     1681 ccttccgtct tacctcaatg gggtcatgcc cccgacacag agctttgccc ctgatcccaa
     1741 gtatgtcagc agcaaagcac tgcaaagaca gaacagcgaa ggatctgcca gtaaggttcc
     1801 ttgcattctg cccattattg aaaatgggaa gaaggttagt tcaacacatt attacctact
     1861 acctgaacga ccaccatacc tggacaaata tgaaaaattt tttagggaag cagaagaaac
     1921 aaatggaggc gcccaaatcc agccattacc tgctgactgc ggtatatctt cagccacaga
     1981 aaagccagac tcaaaaacaa aaatggatct gggtggccac gtgaagcgta aacatttatc
     2041 ctatgtggtt tctccttagt tgccaacttt cttgtacaaa gttggcatta taagaaagca
     2101 ttgcttatca atttgttgca acgaacaggt cactatcagt caaaataaaa tcattatttg
     2161 ccatccagct gatatcccct atagtgagtc gtattacatg gtcatagctg tttcctggca
     2221 gctctggccc gtgtctcaaa atctctgatg ttacattgca caagataaaa atatatcatc
     2281 atgcctcctc tagaccagcc aggacagaaa tgcctcgact tcgctgctac ccaaggttgc
     2341 cgggtgacgc acaccgtgga aacggatgaa ggcacgaacc cagtggacat aagcctgttc
     2401 ggttcgtaag ctgtaatgca agtagcgtat gcgctcacgc aactggtcca gaaccttgac
     2461 cgaacgcagc ggtggtaacg gcgcagtggc ggttttcatg gcttgttatg actgtttttt
     2521 tggggtacag tctatgcctc gggcatccaa gcagcaagcg cgttacgccg tgggtcgatg
     2581 tttgatgtta tggagcagca acgatgttac gcagcagggc agtcgcccta aaacaaagtt
     2641 aaacattatg agggaagcgg tgatcgccga agtatcgact caactatcag aggtagttgg
     2701 cgtcatcgag cgccatctcg aaccgacgtt gctggccgta catttgtacg gctccgcagt
     2761 ggatggcggc ctgaagccac acagtgatat tgatttgctg gttacggtga ccgtaaggct
     2821 tgatgaaaca acgcggcgag ctttgatcaa cgaccttttg gaaacttcgg cttcccctgg
     2881 agagagcgag attctccgcg ctgtagaagt caccattgtt gtgcacgacg acatcattcc
     2941 gtggcgttat ccagctaagc gcgaactgca atttggagaa tggcagcgca atgacattct
     3001 tgcaggtatc ttcgagccag ccacgatcga cattgatctg gctatcttgc tgacaaaagc
     3061 aagagaacat agcgttgcct tggtaggtcc agcggcggag gaactctttg atccggttcc
     3121 tgaacaggat ctatttgagg cgctaaatga aaccttaacg ctatggaact cgccgcccga
     3181 ctgggctggc gatgagcgaa atgtagtgct tacgttgtcc cgcatttggt acagcgcagt
     3241 aaccggcaaa atcgcgccga aggatgtcgc tgccgactgg gcaatggagc gcctgccggc
     3301 ccagtatcag cccgtcatac ttgaagctag acaggcttat cttggacaag aagaagatcg
     3361 cttggcctcg cgcgcagatc agttggaaga atttgtccac tacgtgaaag gcgagatcac
     3421 caaggtagtc ggcaaataac cctcgagcca cccatgacca aaatccctta acgtgagtta
     3481 cgcgtcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
     3541 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
     3601 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
     3661 gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc
     3721 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
     3781 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
     3841 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
     3901 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
     3961 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
     4021 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
     4081 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
     4141 tttacggttc ctggcctttt gctggccttt tgctcacatg tt
//
