LOCUS       sequence-164610-        5208 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing FGFR3 , part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5208)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 5208)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_FGFR3_WT
FEATURES             Location/Qualifiers
     source          1..5208
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(3087..3186)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(3203..3222)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3204..3222)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3227..3243)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3227..3243)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             3674..4465
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      4558..5146
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5047..5066
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agttggcatg ggcgcccctg cctgcgccct cgcgctctgc gtggccgtgg ccatcgtggc
      721 cggcgcctcc tcggagtcct tggggacgga gcagcgcgtc gtggggcgag cggcagaagt
      781 cccgggccca gagcccggcc agcaggagca gttggtcttc ggcagcgggg atgctgtgga
      841 gctgagctgt cccccgcccg ggggtggtcc catggggccc actgtctggg tcaaggatgg
      901 cacagggctg gtgccctcgg agcgtgtcct ggtggggccc cagcggctgc aggtgctgaa
      961 tgcctcccac gaggactccg gggcctacag ctgccggcag cggctcacgc agcgcgtact
     1021 gtgccacttc agtgtgcggg tgacagacgc tccatcctcg ggagatgacg aagacgggga
     1081 ggacgaggct gaggacacag gtgtggacac aggggcccct tactggacac ggcccgagcg
     1141 gatggacaag aagctgctgg ccgtgccggc cgccaacacc gtccgcttcc gctgcccagc
     1201 cgctggcaac cccactccct ccatctcctg gctgaagaac ggcagggagt tccgcggcga
     1261 gcaccgcatt ggaggcatca agctgcggca tcagcagtgg agcctggtca tggaaagcgt
     1321 ggtgccctcg gaccgcggca actacacctg cgtcgtggag aacaagtttg gcagcatccg
     1381 gcagacgtac acgctggacg tgctggagcg ctccccgcac cggcccatcc tgcaggcggg
     1441 gctgccggcc aaccagacgg cggtgctggg cagcgacgtg gagttccact gcaaggtgta
     1501 cagtgacgca cagccccaca tccagtggct caagcacgtg gaggtgaatg gcagcaaggt
     1561 gggcccggac ggcacaccct acgttaccgt gctcaagacg gcgggcgcta acaccaccga
     1621 caaggagcta gaggttctct ccttgcacaa cgtcaccttt gaggacgccg gggagtacac
     1681 ctgcctggcg ggcaattcta ttgggttttc tcatcactct gcgtggctgg tggtgctgcc
     1741 agccgaggag gagctggtgg aggctgacga ggcgggcagt gtgtatgcag gcatcctcag
     1801 ctacggggtg ggcttcttcc tgttcatcct ggtggtggcg gctgtgacgc tctgccgcct
     1861 gcgcagcccc cccaagaaag gcctgggctc ccccaccgtg cacaagatct cccgcttccc
     1921 gctcaagcga caggtgtccc tggagtccaa cgcgtccatg agctccaaca caccactggt
     1981 gcgcatcgca aggctgtcct caggggaggg ccccacgctg gccaatgtct ccgagctcga
     2041 gctgcctgcc gaccccaaat gggagctgtc tcgggcccgg ctgaccctgg gcaagcccct
     2101 tggggagggc tgcttcggcc aggtggtcat ggcggaggcc atcggcattg acaaggaccg
     2161 ggccgccaag cctgtcaccg tagccgtgaa gatgctgaaa gacgatgcca ctgacaagga
     2221 cctgtcggac ctggtgtctg agatggagat gatgaagatg atcgggaaac acaaaaacat
     2281 catcaacctg ctgggcgcct gcacgcaggg cgggcccctg tacgtgctgg tggagtacgc
     2341 ggccaagggt aacctgcggg agtttctgcg ggcgcggcgg cccccgggcc tggactactc
     2401 cttcgacacc tgcaagccgc ccgaggagca gctcaccttc aaggacctgg tgtcctgtgc
     2461 ctaccaggtg gcccggggca tggagtactt ggcctcccag aagtgcatcc acagggacct
     2521 ggctgcccgc aatgtgctgg tgaccgagga caacgtgatg aagatcgcag acttcgggct
     2581 ggcccgggac gtgcacaacc tcgactacta caagaagaca accaacggcc ggctgcccgt
     2641 gaagtggatg gcgcctgagg ccttgtttga ccgagtctac actcaccaga gtgacgtctg
     2701 gtcctttggg gtcctgctct gggagatctt cacgctgggg ggctccccgt accccggcat
     2761 ccctgtggag gagctcttca agctgctgaa ggagggccac cgcatggaca agcccgccaa
     2821 ctgcacacac gacctgtaca tgatcatgcg ggagtgctgg catgccgcgc cctcccagag
     2881 gcccaccttc aagcagctgg tggaggacct ggaccgtgtc cttaccgtga cgtccaccga
     2941 cgagtacctg gacctgtcgg cgcctttcga gcagtactcc ccgggtggcc aggacacccc
     3001 cagctccagc tcctcagggg acgactccgt gtttgcccac gacctgctgc ccccggcccc
     3061 acccagcagt gggggctcgc ggacgtgacc aactttcttg tacaaagttg gcattataag
     3121 aaagcattgc ttatcaattt gttgcaacga acaggtcact atcagtcaaa ataaaatcat
     3181 tatttgccat ccagctgata tcccctatag tgagtcgtat tacatggtca tagctgtttc
     3241 ctggcagctc tggcccgtgt ctcaaaatct ctgatgttac attgcacaag ataaaaatat
     3301 atcatcatgc ctcctctaga ccagccagga cagaaatgcc tcgacttcgc tgctacccaa
     3361 ggttgccggg tgacgcacac cgtggaaacg gatgaaggca cgaacccagt ggacataagc
     3421 ctgttcggtt cgtaagctgt aatgcaagta gcgtatgcgc tcacgcaact ggtccagaac
     3481 cttgaccgaa cgcagcggtg gtaacggcgc agtggcggtt ttcatggctt gttatgactg
     3541 tttttttggg gtacagtcta tgcctcgggc atccaagcag caagcgcgtt acgccgtggg
     3601 tcgatgtttg atgttatgga gcagcaacga tgttacgcag cagggcagtc gccctaaaac
     3661 aaagttaaac attatgaggg aagcggtgat cgccgaagta tcgactcaac tatcagaggt
     3721 agttggcgtc atcgagcgcc atctcgaacc gacgttgctg gccgtacatt tgtacggctc
     3781 cgcagtggat ggcggcctga agccacacag tgatattgat ttgctggtta cggtgaccgt
     3841 aaggcttgat gaaacaacgc ggcgagcttt gatcaacgac cttttggaaa cttcggcttc
     3901 ccctggagag agcgagattc tccgcgctgt agaagtcacc attgttgtgc acgacgacat
     3961 cattccgtgg cgttatccag ctaagcgcga actgcaattt ggagaatggc agcgcaatga
     4021 cattcttgca ggtatcttcg agccagccac gatcgacatt gatctggcta tcttgctgac
     4081 aaaagcaaga gaacatagcg ttgccttggt aggtccagcg gcggaggaac tctttgatcc
     4141 ggttcctgaa caggatctat ttgaggcgct aaatgaaacc ttaacgctat ggaactcgcc
     4201 gcccgactgg gctggcgatg agcgaaatgt agtgcttacg ttgtcccgca tttggtacag
     4261 cgcagtaacc ggcaaaatcg cgccgaagga tgtcgctgcc gactgggcaa tggagcgcct
     4321 gccggcccag tatcagcccg tcatacttga agctagacag gcttatcttg gacaagaaga
     4381 agatcgcttg gcctcgcgcg cagatcagtt ggaagaattt gtccactacg tgaaaggcga
     4441 gatcaccaag gtagtcggca aataaccctc gagccaccca tgaccaaaat cccttaacgt
     4501 gagttacgcg tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
     4561 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     4621 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
     4681 cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa
     4741 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     4801 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     4861 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     4921 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
     4981 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     5041 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
     5101 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
     5161 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgtt
//
