LOCUS       sequence-164613-        5178 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing FURIN , part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5178)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 5178)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_FURIN_WT
FEATURES             Location/Qualifiers
     source          1..5178
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     regulatory      1968..1977
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    complement(3057..3156)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(3173..3192)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3174..3192)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3197..3213)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3197..3213)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             3644..4435
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      4528..5116
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5017..5036
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agttggcacc atggagctga ggccctggtt gctatgggtg gtagcagcaa caggaacctt
      721 ggtcctgcta gcagctgatg ctcagggcca gaaggtcttc accaacacgt gggctgtgcg
      781 catccctgga ggcccagcgg tggccaacag tgtggcacgg aagcatgggt tcctcaacct
      841 gggccagatc ttcggggact attaccactt ctggcatcga ggagtgacga agcggtccct
      901 gtcgcctcac cgcccgcggc acagccggct gcagagggag cctcaagtac agtggctgga
      961 acagcaggtg gcaaagcgac ggactaaacg ggacgtgtac caggagccca cagaccccaa
     1021 gtttcctcag cagtggtacc tgtctggtgt cactcagcgg gacctgaatg tgaaggcggc
     1081 ctgggcgcag ggctacacag ggcacggcat tgtggtctcc attctggacg atggcatcga
     1141 gaagaaccac ccggacttgg caggcaatta tgatcctggg gccagttttg atgtcaatga
     1201 ccaggaccct gacccccagc ctcggtacac acagatgaat gacaacaggc acggcacacg
     1261 gtgtgcgggg gaagtggctg cggtggccaa caacggtgtc tgtggtgtag gtgtggccta
     1321 caacgcccgc attggagggg tgcgcatgct ggatggcgag gtgacagatg cagtggaggc
     1381 acgctcgctg ggcctgaacc ccaaccacat ccacatctac agtgccagct ggggccccga
     1441 ggatgacggc aagacagtgg atgggccagc ccgcctcgcc gaggaggcct tcttccgtgg
     1501 ggttagccag ggccgagggg ggctgggctc catctttgtc tgggcctcgg ggaacggggg
     1561 ccgggaacat gacagctgca actgcgacgg ctacaccaac agtatctaca cgctgtccat
     1621 cagcagcgcc acgcagtttg gcaacgtgcc gtggtacagc gaggcctgct cgtccacact
     1681 ggccacgacc tacagcagtg gcaaccagaa tgagaagcag atcgtgacga ctgacttgcg
     1741 gcagaagtgc acggagtctc acacgggcac ctcagcctct gcccccttag cagccggcat
     1801 cattgctctc accctggagg ccaataagaa cctcacatgg cgggacatgc aacacctggt
     1861 ggtacagacc tcgaagccag cccacctcaa tgccaacgac tgggccacca atggtgtggg
     1921 ccggaaagtg agccactcat atggctacgg gcttttggac gcaggcgcca tggtggccct
     1981 ggcccagaat tggaccacag tggcccccca gcggaagtgc atcatcgaca tcctcaccga
     2041 gcccaaagac atcgggaaac ggctcgaggt gcggaagacc gtgaccgcgt gcctgggcga
     2101 gcccaaccac atcactcggc tggagcacgc tcaggcgcgg ctcaccctgt cctataatcg
     2161 ccgtggcgac ctggccatcc acctggtcag ccccatgggc acccgctcca ccctgctggc
     2221 agccaggcca catgactact ccgcagatgg gtttaatgac tgggccttca tgacaactca
     2281 ttcctgggat gaggatccct ctggcgagtg ggtcctagag attgaaaaca ccagcgaagc
     2341 caacaactat gggacgctga ccaagttcac cctcgtactc tatggcaccg cccctgaggg
     2401 gctgcccgta cctccagaaa gcagtggctg caagaccctc acgtccagtc aggcctgtgt
     2461 ggtgtgcgag gaaggcttct ccctgcacca gaagagctgt gtccagcact gccctccagg
     2521 cttcgccccc caagtcctcg atacgcacta tagcaccgag aatgacgtgg agaccatccg
     2581 ggccagcgtc tgcgccccct gccacgcctc atgtgccaca tgccaggggc cggccctgac
     2641 agactgcctc agctgcccca gccacgcctc cttggaccct gtggagcaga cttgctcccg
     2701 gcaaagccag agcagccgag agtccccgcc acagcagcag ccacctcggc tgcccccgga
     2761 ggtggaggcg gggcaacggc tgcgggcagg gctgctgccc tcacacctgc ctgaggtggt
     2821 ggccggcctc agctgcgcct tcatcgtgct ggtcttcgtc actgtcttcc tggtcctgca
     2881 gctgcgctct ggctttagtt ttcggggggt gaaggtgtac accatggacc gtggcctcat
     2941 ctcctacaag gggctgcccc ctgaagcctg gcaggaggag tgcccgtctg actcagaaga
     3001 ggacgagggc cggggcgaga ggaccgcctt tatcaaagac cagagcgccc tctgattgcc
     3061 aactttcttg tacaaagttg gcattataag aaagcattgc ttatcaattt gttgcaacga
     3121 acaggtcact atcagtcaaa ataaaatcat tatttgccat ccagctgata tcccctatag
     3181 tgagtcgtat tacatggtca tagctgtttc ctggcagctc tggcccgtgt ctcaaaatct
     3241 ctgatgttac attgcacaag ataaaaatat atcatcatgc ctcctctaga ccagccagga
     3301 cagaaatgcc tcgacttcgc tgctacccaa ggttgccggg tgacgcacac cgtggaaacg
     3361 gatgaaggca cgaacccagt ggacataagc ctgttcggtt cgtaagctgt aatgcaagta
     3421 gcgtatgcgc tcacgcaact ggtccagaac cttgaccgaa cgcagcggtg gtaacggcgc
     3481 agtggcggtt ttcatggctt gttatgactg tttttttggg gtacagtcta tgcctcgggc
     3541 atccaagcag caagcgcgtt acgccgtggg tcgatgtttg atgttatgga gcagcaacga
     3601 tgttacgcag cagggcagtc gccctaaaac aaagttaaac attatgaggg aagcggtgat
     3661 cgccgaagta tcgactcaac tatcagaggt agttggcgtc atcgagcgcc atctcgaacc
     3721 gacgttgctg gccgtacatt tgtacggctc cgcagtggat ggcggcctga agccacacag
     3781 tgatattgat ttgctggtta cggtgaccgt aaggcttgat gaaacaacgc ggcgagcttt
     3841 gatcaacgac cttttggaaa cttcggcttc ccctggagag agcgagattc tccgcgctgt
     3901 agaagtcacc attgttgtgc acgacgacat cattccgtgg cgttatccag ctaagcgcga
     3961 actgcaattt ggagaatggc agcgcaatga cattcttgca ggtatcttcg agccagccac
     4021 gatcgacatt gatctggcta tcttgctgac aaaagcaaga gaacatagcg ttgccttggt
     4081 aggtccagcg gcggaggaac tctttgatcc ggttcctgaa caggatctat ttgaggcgct
     4141 aaatgaaacc ttaacgctat ggaactcgcc gcccgactgg gctggcgatg agcgaaatgt
     4201 agtgcttacg ttgtcccgca tttggtacag cgcagtaacc ggcaaaatcg cgccgaagga
     4261 tgtcgctgcc gactgggcaa tggagcgcct gccggcccag tatcagcccg tcatacttga
     4321 agctagacag gcttatcttg gacaagaaga agatcgcttg gcctcgcgcg cagatcagtt
     4381 ggaagaattt gtccactacg tgaaaggcga gatcaccaag gtagtcggca aataaccctc
     4441 gagccaccca tgaccaaaat cccttaacgt gagttacgcg tcgttccact gagcgtcaga
     4501 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
     4561 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
     4621 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct
     4681 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     4741 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
     4801 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
     4861 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
     4921 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
     4981 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
     5041 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
     5101 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
     5161 gccttttgct cacatgtt
//
