LOCUS       sequence-16463-e         736 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 736)
  AUTHORS   Scheibel T, Lindquist SL
  TITLE     The role of conformational flexibility in prion propagation and 
            maintenance for Sup35p.
  JOURNAL   Nat Struct Biol 2001 Nov;8(11):958-62.
  PUBMED    11685242
REFERENCE   2  (bases 1 to 736)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Struct 
            Biol"; date: "2001-11"; volume: "8(11)"; pages: "958-62"
COMMENT     Sequence Label: pGEX-2T-M
FEATURES             Location/Qualifiers
     source          1..736
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             8..25
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     primer_bind     complement(480..502)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(647..665)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 atcggatctg gttccgcgtg gatccatgtc tttgaacgac tttcaaaagc aacaaaagca
       61 ggccgctccc aaaccaaaga agactttgaa gcttgtctcc agttccggta tcaagttggc
      121 caatgctacc aagaaggttg gcacaaaacc tgccgaatct gataagaaag aggaagagaa
      181 gtctgctgaa accaaagaac caactaaaga gccaacaaag gtcgaagaac cagttaaaaa
      241 ggaggagaaa ccagtccaga ctgaagaaaa gacggaggaa aaatcggaac ttccaaaggt
      301 agaagacctt aaaatctctg aatcaacaca taataccaac aatgccaatg ttaccagtgc
      361 tgatgccttg atcaaggaac aggaagaaga agtggatgac gaagttgtta acgattaata
      421 ggaattcatc gtgactgact gacgatctgc ctcgcgcgtt tcggtgatga cggtgaaaac
      481 ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc
      541 agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggggcgc agccatgacc
      601 cagtcacgta gcgatagcgg agtgtataat tcttgaagac gaaagggcct cgtgatacgc
      661 ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt
      721 ccgggaaatg tgcgcg
//
