LOCUS       sequence-164643-         926 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  guide RNA expression lentiviral construct.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 926)
  AUTHORS   Liu XS, Wu H, Ji X, Stelzer Y, Wu X, Czauderna S, Shu J, Dadon D, 
            Young RA, Jaenisch R
  TITLE     Editing DNA Methylation in the Mammalian Genome.
  JOURNAL   Cell. 2016 Sep 22;167(1):233-247.e17. doi: 
            10.1016/j.cell.2016.08.056.
  PUBMED    27662091
REFERENCE   2  (bases 1 to 926)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2016.08"; journalName: "Cell"; date: "2016-09-22- 
            22"; volume: "167"; issue: "1"; pages: "233-247.e17"
COMMENT     Sequence Label: pgRNA-modified
FEATURES             Location/Qualifiers
     source          1..926
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(192..225)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     229..249
                     /label=mU6-F
                     /note="Mouse U6 promoter, forward primer"
     misc_RNA        341..416
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     enhancer        541..844
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 ccgacgcgcc atctctaggc ccgcgccggc cccctcgcac ggacttgtgg gagaagctcg
       61 gctactcccc tgccccggtt aatttgcata taatatttcc tagtaactat agaggcttaa
      121 tgtgcgataa aagacagata atctgttctt tttaatacta gctacatttt acatgatagg
      181 cttggatttc tataacttcg tatagcatac attatacgaa gttataaaca gcacaaaagg
      241 aaactcaccc taactgtaaa gtaattgtgt gttttgagac tataagtatc ccttggagaa
      301 ccaccttgtt ggtctcgcag gtgggatccc acctgctctc gttttagagc tagaaatagc
      361 aagttaaaat aaggctagtc cgttatcaac ttgaaaaagt ggcaccgagt cggtgctttt
      421 tttctcgagt actaggatcc attaggcggc cgcgtggata accgtattac cgccatgcat
      481 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
      541 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      601 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      661 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      721 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      781 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      841 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      901 atttccaagt ctccacccca ttgacg
//
