LOCUS       sequence-164647-        6114 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing GLI1 , part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6114)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 6114)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_GLI1_WT
FEATURES             Location/Qualifiers
     source          1..6114
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(3993..4092)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(4109..4128)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4110..4128)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4133..4149)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4133..4149)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             4580..5371
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      5464..6052
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5953..5972
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agttggcacc atgttcaact cgatgacccc accaccaatc agtagctatg gcgagccctg
      721 ctgtctccgg cccctcccca gtcagggggc ccccagtgtg gggacagaag gactgtctgg
      781 cccgcccttc tgccaccaag ctaacctcat gtccggcccc cacagttatg ggccagccag
      841 agagaccaac agctgcaccg agggcccact cttttcttct ccccggagtg cagtcaagtt
      901 gaccaagaag cgggcactgt ccatctcacc tctgtcggat gccagcctgg acctgcagac
      961 ggttatccgc acctcaccca gctccctcgt agctttcatc aactcgcgat gcacatctcc
     1021 aggaggctcc tacggtcatc tctccattgg caccatgagc ccatctctgg gattcccagc
     1081 ccagatgaat caccaaaaag ggccctcgcc ttcctttggg gtccagcctt gtggtcccca
     1141 tgactctgcc cggggtggga tgatcccaca tcctcagtcc cggggaccct tcccaacttg
     1201 ccagctgaag tctgagctgg acatgctggt tggcaagtgc cgggaggaac ccttggaagg
     1261 tgatatgtcc agccccaact ccacaggcat acaggatccc ctgttgggga tgctggatgg
     1321 gcgggaggac ctcgagagag aggagaagcg tgagcctgaa tctgtgtatg aaactgactg
     1381 ccgttgggat ggctgcagcc aggaatttga ctcccaagag cagctggtgc accacatcaa
     1441 cagcgagcac atccacgggg agcggaagga gttcgtgtgc cactgggggg gctgctccag
     1501 ggagctgagg cccttcaaag cccagtacat gctggtggtt cacatgcgca gacacactgg
     1561 cgagaagcca cacaagtgca cgtttgaagg gtgccggaag tcatactcac gcctcgaaaa
     1621 cctgaagacg cacctgcggt cacacacggg tgagaagcca tacatgtgtg agcacgaggg
     1681 ctgcagtaaa gccttcagca atgccagtga ccgagccaag caccagaatc ggacccattc
     1741 caatgagaag ccgtatgtat gtaagctccc tggctgcacc aaacgctata cagatcctag
     1801 ctcgctgcga aaacatgtca agacagtgca tggtcctgac gcccatgtga ccaaacggca
     1861 ccgtggggat ggccccctgc ctcgggcacc atccatttct acagtggagc ccaagaggga
     1921 gcgggaagga ggtcccatca gggaggaaag cagactgact gtgccagagg gtgccatgaa
     1981 gccacagcca agccctgggg cccagtcatc ctgcagcagt gaccactccc cggcagggag
     2041 tgcagccaat acagacagtg gtgtggaaat gactggcaat gcagggggca gcactgaaga
     2101 cctctccagc ttggacgagg gaccttgcat tgctggcact ggtctgtcca ctcttcgccg
     2161 ccttgagaac ctcaggctgg accagctaca tcaactccgg ccaataggga cccggggtct
     2221 caaactgccc agcttgtccc acaccggtac cactgtgtcc cgccgcgtgg gccccccagt
     2281 ctctcttgaa cgccgcagca gcagctccag cagcatcagc tctgcctata ctgtcagccg
     2341 ccgctcctcc ctggcctctc ctttcccccc tggctcccca ccagagaatg gagcatcctc
     2401 cctgcctggc cttatgcctg cccagcacta cctgcttcgg gcaagatatg cttcagccag
     2461 agggggtggt acttcgccca ctgcagcatc cagcctggat cggataggtg gtcttcccat
     2521 gcctccttgg agaagccgag ccgagtatcc aggatacaac cccaatgcag gggtcacccg
     2581 gagggccagt gacccagccc aggctgctga ccgtcctgct ccagctagag tccagaggtt
     2641 caagagcctg ggctgtgtcc ataccccacc cactgtggca gggggaggac agaactttga
     2701 tccttacctc ccaacctctg tctactcacc acagcccccc agcatcactg agaatgctgc
     2761 catggatgct agagggctac aggaagagcc agaagttggg acctccatgg tgggcagtgg
     2821 tctgaacccc tatatggact tcccacctac tgatactctg ggatatgggg gacctgaagg
     2881 ggcagcagct gagccttatg gagcgagggg tccaggctct ctgcctcttg ggcctggtcc
     2941 acccaccaac tatggcccca acccctgtcc ccagcaggcc tcatatcctg accccaccca
     3001 agaaacatgg ggtgagttcc cttcccactc tgggctgtac ccaggcccca aggctctagg
     3061 tggaacctac agccagtgtc ctcgacttga acattatgga caagtgcaag tcaagccaga
     3121 acaggggtgc ccagtggggt ctgactccac aggactggca ccctgcctca atgcccaccc
     3181 cagtgagggg cccccacatc cacagcctct cttttcccat tacccccagc cctctcctcc
     3241 ccaatatctc cagtcaggcc cctataccca gccaccccct gattatcttc cttcagaacc
     3301 caggccttgc ctggactttg attcccccac ccattccaca gggcagctca aggctcagct
     3361 tgtgtgtaat tatgttcaat ctcaacagga gctactgtgg gagggtgggg gcagggaaga
     3421 tgcccccgcc caggaacctt cctaccagag tcccaagttt ctggggggtt cccaggttag
     3481 cccaagccgt gctaaagctc cagtgaacac atatggacct ggctttggac ccaacttgcc
     3541 caatcacaag tcaggttcct atcccacccc ttcaccatgc catgaaaatt ttgtagtggg
     3601 ggcaaatagg gcttcacata gggcagcagc accacctcga cttctgcccc cattgcccac
     3661 ttgctatggg cctctcaaag tgggaggcac aaaccccagc tgtggtcatc ctgaggtggg
     3721 caggctagga gggggtcctg ccttgtaccc tcctcccgaa ggacaggtat gtaaccccct
     3781 ggactctctt gatcttgaca acactcagct ggactttgtg gctattctgg atgagcccca
     3841 ggggctgagt cctcctcctt cccatgatca gcggggcagc tctggacata ccccacctcc
     3901 ctctgggccc cccaacatgg ctgtgggcaa catgagtgtc ttactgagat ccctacctgg
     3961 ggaaacagaa ttcctcaact ctagtgccta attgccaact ttcttgtaca aagttggcat
     4021 tataagaaag cattgcttat caatttgttg caacgaacag gtcactatca gtcaaaataa
     4081 aatcattatt tgccatccag ctgatatccc ctatagtgag tcgtattaca tggtcatagc
     4141 tgtttcctgg cagctctggc ccgtgtctca aaatctctga tgttacattg cacaagataa
     4201 aaatatatca tcatgcctcc tctagaccag ccaggacaga aatgcctcga cttcgctgct
     4261 acccaaggtt gccgggtgac gcacaccgtg gaaacggatg aaggcacgaa cccagtggac
     4321 ataagcctgt tcggttcgta agctgtaatg caagtagcgt atgcgctcac gcaactggtc
     4381 cagaaccttg accgaacgca gcggtggtaa cggcgcagtg gcggttttca tggcttgtta
     4441 tgactgtttt tttggggtac agtctatgcc tcgggcatcc aagcagcaag cgcgttacgc
     4501 cgtgggtcga tgtttgatgt tatggagcag caacgatgtt acgcagcagg gcagtcgccc
     4561 taaaacaaag ttaaacatta tgagggaagc ggtgatcgcc gaagtatcga ctcaactatc
     4621 agaggtagtt ggcgtcatcg agcgccatct cgaaccgacg ttgctggccg tacatttgta
     4681 cggctccgca gtggatggcg gcctgaagcc acacagtgat attgatttgc tggttacggt
     4741 gaccgtaagg cttgatgaaa caacgcggcg agctttgatc aacgaccttt tggaaacttc
     4801 ggcttcccct ggagagagcg agattctccg cgctgtagaa gtcaccattg ttgtgcacga
     4861 cgacatcatt ccgtggcgtt atccagctaa gcgcgaactg caatttggag aatggcagcg
     4921 caatgacatt cttgcaggta tcttcgagcc agccacgatc gacattgatc tggctatctt
     4981 gctgacaaaa gcaagagaac atagcgttgc cttggtaggt ccagcggcgg aggaactctt
     5041 tgatccggtt cctgaacagg atctatttga ggcgctaaat gaaaccttaa cgctatggaa
     5101 ctcgccgccc gactgggctg gcgatgagcg aaatgtagtg cttacgttgt cccgcatttg
     5161 gtacagcgca gtaaccggca aaatcgcgcc gaaggatgtc gctgccgact gggcaatgga
     5221 gcgcctgccg gcccagtatc agcccgtcat acttgaagct agacaggctt atcttggaca
     5281 agaagaagat cgcttggcct cgcgcgcaga tcagttggaa gaatttgtcc actacgtgaa
     5341 aggcgagatc accaaggtag tcggcaaata accctcgagc cacccatgac caaaatccct
     5401 taacgtgagt tacgcgtcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
     5461 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
     5521 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
     5581 cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca
     5641 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
     5701 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
     5761 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
     5821 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
     5881 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
     5941 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
     6001 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
     6061 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgtt
//
