LOCUS       sequence-164664-       14795 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentivirus vector. Expresses Halotag-Cbx4 fusion proteins in 
            mammalian cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14795)
  TITLE     pTRIPZ-MSC1-Halotag-Cbx2
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 14795)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pTRIPZ (M)-HT-Cbx4
FEATURES             Location/Qualifiers
     source          1..14795
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2063..2180
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(2245..2619)
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     regulatory      2616..2625
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(2629..2647)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(2639..2686)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     protein_bind    2722..2740
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2757..2775
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2793..2811
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2829..2847
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2864..2882
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2900..2918
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        2930..2968
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2965..2989
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      3008..3017
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3014..3904
                     /codon_start=1
                     /product="modified bacterial dehalogenase that forms 
                     covalent bonds with chloroalkane derivatives"
                     /label=HaloTag(R)
                     /translation="MAEIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPTSS
                     YVWRNIIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVI
                     HDWGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLII
                     DQNVFIEGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIVALV
                     EEYMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGPGLNLLQEDNPDL
                     IGSEIARWLSTLEISG"
     CDS             5084..5113
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             5087..5116
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             5090..5119
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     promoter        5613..6824
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     6661..6680
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     CDS             6843..7550
                     /codon_start=1
                     /product="reverse tetracycline transactivator 3"
                     /label=rtTA3
                     /note="activates expression from Tet-responsive promoters 
                     in the presence of doxycycline"
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPG"
     misc_feature    7573..8145
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(7740..7757)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     7967..7986
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             8148..8750
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MATEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIE
                     RVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGSRMAELSGSRL
                     AAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLET
                     SAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     8647..8667
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    8766..9354
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8819..8839)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9237..9248)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             9561..9794
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(9895..9912)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    9901..10125
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      10171..10599
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10258..10277)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10468..10489
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(10608..10628)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        10613..10942
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      10793..10928
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     10855..10874
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     polyA_signal    12150..12271
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(12320..12336)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(12320..12336)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(12333..12355)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    12344..12360
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(12368..12374,12375..12392,12393..12398))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12413..12434
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(12551..12568)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(12722..13310)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(12802..12821)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(13481..14272,14273..14341))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     14104..14123
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(14342..14446)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    14494..14628
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atcggcactg cgtgcgccaa ttctgcagac
     2041 aaatggcagt attcatccac aattttaaaa gaaaaggggg gattgggggg tacagtgcag
     2101 gggaaagaat agtagacata atagcaacag acatacaaac taaagaatta caaaaacaaa
     2161 ttacaaaaat tcaaaatttt cgggtttatt acagggacag cagagatcca gtttggttag
     2221 taccgggccc gctctagtcc ggaatcagtc ctgctcctcg gccacgaagt gcacgcagtt
     2281 gccggccggg tcgcgcaggg cgaactcccg cccccacggc tgctcgccga tctcggtcat
     2341 ggccggcccg gaggcgtccc ggaagttcgt ggacacgacc tccgaccact cggcgtacag
     2401 ctcgtccagg ccgcgcaccc acacccaggc cagggtgttg tccggcacca cctggtcctg
     2461 gaccgcgctg atgaacaggg tcacgtcgtc ccggaccaca ccggcgaagt cgtcctccac
     2521 gaagtcccgg gagaacccga gccggtcggt ccagaactcg accgctccgg cgacgtcgcg
     2581 cgcggtgagc accggaacgg cactggtcaa cttggccatg gtggccctcc tatagtgagt
     2641 cgtattatac tatgccgata tactatgccg atgattaatt gtcaacacgt gctgcaggtc
     2701 cgaggttcta gacgagttta ctccctatca gtgatagaga acgatgtcga gtttactccc
     2761 tatcagtgat agagaacgta tgtcgagttt actccctatc agtgatagag aacgtatgtc
     2821 gagtttactc cctatcagtg atagagaacg tatgtcgagt ttatccctat cagtgataga
     2881 gaacgtatgt cgagtttact ccctatcagt gatagagaac gtatgtcgag gtaggcgtgt
     2941 acggtgggag gcctatataa gcagagctcg tttagtgaac cgtcagatcg caccggtccg
     3001 ctcgagggcc accatggcag aaatcggtac tggctttcca ttcgaccccc attatgtgga
     3061 agtcctgggc gagcgcatgc actacgtcga tgttggtccg cgcgatggca cccctgtgct
     3121 gttcctgcac ggtaacccga cctcctccta cgtgtggcgc aacatcatcc cgcatgttgc
     3181 accgacccat cgctgcattg ctccagacct gatcggtatg ggcaaatccg acaaaccaga
     3241 cctgggttat ttcttcgacg accacgtccg cttcatggat gccttcatcg aagccctggg
     3301 tctggaagag gtcgtcctgg tcattcacga ctggggctcc gctctgggtt tccactgggc
     3361 caagcgcaat ccagagcgcg tcaaaggtat tgcatttatg gagttcatcc gccctatccc
     3421 gacctgggac gaatggccag aatttgcccg cgagaccttc caggccttcc gcaccaccga
     3481 cgtcggccgc aagctgatca tcgatcagaa cgtttttatc gagggtacgc tgccgatggg
     3541 tgtcgtccgc ccgctgactg aagtcgagat ggaccattac cgcgagccgt tcctgaatcc
     3601 tgttgaccgc gagccactgt ggcgcttccc aaacgagctg ccaatcgccg gtgagccagc
     3661 gaacatcgtc gcgctggtcg aagaatacat ggactggctg caccagtccc ctgtcccgaa
     3721 gctgctgttc tggggcaccc caggcgttct gatcccaccg gccgaagccg ctcgcctggc
     3781 caaaagcctg cctaactgca aggctgtgga catcggcccg ggtctgaatc tgctgcaaga
     3841 agacaacccg gacctgatcg gcagcgagat cgcgcgctgg ctgtcgacgc tcgagatttc
     3901 cggcgcgaat tcacttgcta tggagctgcc agctgttggc gagcacgtct tcgcggtgga
     3961 aagcttcgag aagaagcgga tccgcaaggg cagagtggag tatctggtga aatggagagg
     4021 ctggtcgccc aaatataaca cgtgggaacc ggaggagaac atcctggacc ccaggctgct
     4081 gatcgccttc cagaacaggg aacggcagga gcagctgatg ggatatcgga agagagggcc
     4141 gaagcccaaa ccgctagtgg tgcaggtgcc tacctttgcc cgtcgttcca atgtcctgac
     4201 cggcctccag gactcctcca ctgacaaccg tgccaagctg gatttgggcg cgcaggggaa
     4261 gggccagggg catcagtacg agctcaacag caagaagcac caccagtacc agccgcacag
     4321 caaggagcgg gcgggcaagc ccccgccgcc gggcaagagc ggcaagtact actaccagct
     4381 caacagcaag aagcaccacc cctaccagcc cgaccccaaa atgtacgacc tgcagtacca
     4441 gggcggccac aaggaggcgc ccagccccac ctgcccggac ctgggggcca agagccaccc
     4501 gcccgacaag tgggcgcaag gtgcgggggc caaaggctac ctgggggcgg tgaagcccct
     4561 ggccggtgcg gcgggtgctc caggcaaagg ctccgagaag ggccccccca acggaatgat
     4621 gccggccccc aaagaggctg tgacgggcaa cgggattggg ggcaagatga agatagtcaa
     4681 gaacaagaac aagaacggac gcatcgtgat cgtgatgagc aaatacatgg agaacggcat
     4741 gcaggcggtg aagatcaagt ccggcgaggt ggcagagggg gaggctcgct cccccagcca
     4801 caagaagcgg gcagccgacg agcgccaccc tcctgccgac aggactttta aaaaggcggc
     4861 gggcgcagag gagaagaagg tggaggcgcc gcccaagagg agggaggagg aggtgtccgg
     4921 ggttagcgat ccgcagcccc aggatgccgg ctcccgcaag ctgtccccga ccaaggaggc
     4981 ctttggagag cagcccctgc agctcaccac caagcccgac ctgcttgcct gggacccggc
     5041 ccggaacacg cacccgccct cacaccaccc gcacccgcac ccccatcacc accaccacca
     5101 ccaccaccac caccaccacg ccgtcggcct gaatctctcc cacgtgcgca agcgctgcct
     5161 ctccgagacc cacggcgagc gcgagccctg caagaagcgg ctgactgcgc gcagcatcag
     5221 cacccccacc tgcctggggg gcagcccagc cgctgagcgc ccggccgacc tgccaccagc
     5281 cgccgccctc ccgcagcccg aggtcatcct gctagactca gacctggatg aacccataga
     5341 cttgcgctgc gtcaagacgc gcagcgaggc cggggagccg cccagctccc tccaggtgaa
     5401 gcccgagaca ccggcgtcgg cggcggtggc ggtggcggcg gcagcggcac ccaccacgac
     5461 ggcggagaag cctccagccg aggcccagga cgaacctgca gagtcgctga gcgagttcaa
     5521 gcccttcttt gggaatataa ttatcaccga cgtcaccgcg aactgcctca ccgttacttt
     5581 caaggagtac gtgacggtgt aggcggacgc gtggcctccg cgccgggttt tggcgcctcc
     5641 cgcgggcgcc cccctcctca cggcgagcgc tgccacgtca gacgaagggc gcagcgagcg
     5701 tcctgatcct tccgcccgga cgctcaggac agcggcccgc tgctcataag actcggcctt
     5761 agaaccccag tatcagcaga aggacatttt aggacgggac ttgggtgact ctagggcact
     5821 ggttttcttt ccagagagcg gaacaggcga ggaaaagtag tcccttctcg gcgattctgc
     5881 ggagggatct ccgtggggcg gtgaacgccg atgattatat aaggacgcgc cgggtgtggc
     5941 acagctagtt ccgtcgcagc cgggatttgg gtcgcggttc ttgtttgtgg atcgctgtga
     6001 tcgtcacttg gtgagtagcg ggctgctggg ctggccgggg ctttcgtggc cgccgggccg
     6061 ctcggtggga cggaagcgtg tggagagacc gccaagggct gtagtctggg tccgcgagca
     6121 aggttgccct gaactggggg ttggggggag cgcagcaaaa tggcggctgt tcccgagtct
     6181 tgaatggaag acgcttgtga ggcgggctgt gaggtcgttg aaacaaggtg gggggcatgg
     6241 tgggcggcaa gaacccaagg tcttgaggcc ttcgctaatg cgggaaagct cttattcggg
     6301 tgagatgggc tggggcacca tctggggacc ctgacgtgaa gtttgtcact gactggagaa
     6361 ctcggtttgt cgtctgttgc gggggcggca gttatggcgg tgccgttggg cagtgcaccc
     6421 gtacctttgg gagcgcgcgc cctcgtcgtg tcgtgacgtc acccgttctg ttggcttata
     6481 atgcagggtg gggccacctg ccggtaggtg tgcggtaggc ttttctccgt cgcaggacgc
     6541 agggttcggg cctagggtag gctctcctga atcgacaggc gccggacctc tggtgagggg
     6601 agggataagt gaggcgtcag tttctttggt cggttttatg tacctatctt cttaagtagc
     6661 tgaagctccg gttttgaact atgcgctcgg ggttggcgag tgtgttttgt gaagtttttt
     6721 aggcaccttt tgaaatgtaa tcatttgggt caatatgtaa ttttcagtgt tagactagta
     6781 aattgtccgc taaattctgg ccgtttttgg cttttttgtt agacgctagc ggatccgcca
     6841 ccatgtctag gctggacaag agcaaagtca taaacggagc tctggaatta ctcaatggtg
     6901 tcggtatcga aggcctgacg acaaggaaac tcgctcaaaa gctgggagtt gagcagccta
     6961 ccctgtactg gcacgtgaag aacaagcggg ccctgctcga tgccctgcca atcgagatgc
     7021 tggacaggca tcatacccac ttctgccccc tggaaggcga gtcatggcaa gactttctgc
     7081 ggaacaacgc caagtcatac cgctgtgctc tcctctcaca tcgcgacggg gctaaagtgc
     7141 atctcggcac ccgcccaaca gagaaacagt acgaaaccct ggaaaatcag ctcgcgttcc
     7201 tgtgtcagca aggcttctcc ctggagaacg cactgtacgc tctgtccgcc gtgggccact
     7261 ttacactggg ctgcgtattg gaggaacagg agcatcaagt agcaaaagag gaaagagaga
     7321 cacctaccac cgattctatg cccccacttc tgagacaagc aattgagctg ttcgaccggc
     7381 agggagccga acctgccttc cttttcggcc tggaactaat catatgtggc ctggagaaac
     7441 agctaaagtg cgaaagcggc gggccgaccg acgcccttga cgattttgac ttagacatgc
     7501 tcccagccga tgcccttgac gattttgacc ttgacatgct ccccgggtaa ggatccgcgg
     7561 ccgcaaattc cgcccctctc cctccccccc ccctaacgtt actggccgaa gccgcttgga
     7621 ataaggccgg tgtgcgtttg tctatatgtt attttccacc atattgccgt cttttggcaa
     7681 tgtgagggcc cggaaacctg gccctgtctt cttgacgagc attcctaggg gtctttcccc
     7741 tctcgccaaa ggaatgcaag gtctgttgaa tgtcgtgaag gaagcagttc ctctggaagc
     7801 ttcttgaaga caaacaacgt ctgtagcgac cctttgcagg cagcggaacc ccccacctgg
     7861 cgacaggtgc ctctgcggcc aaaagccacg tgtataagat acacctgcaa aggcggcaca
     7921 accccagtgc cacgttgtga gttggatagt tgtggaaaga gtcaaatggc tctcctcaag
     7981 cgtattcaac aaggggctga aggatgccca gaaggtaccc cattgtatgg gatctgatct
     8041 ggggcctcgg tgcacatgct ttacatgtgt ttagtcgagg ttaaaaaaac gtctaggccc
     8101 cccgaaccac ggggacgtgg ttttcctttg aaaaacacga taataccatg gccaccgagt
     8161 acaagcccac ggtgcgcctc gccacccgcg acgacgtccc ccgggccgta cgcaccctcg
     8221 ccgccgcgtt cgccgactac cccgccacgc gccacaccgt cgacccggac cgccacatcg
     8281 agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac atcggcaagg
     8341 tgtgggtcgc ggacgacggc gccgcggtgg cggtctggac cacgccggag agcgtcgaag
     8401 cgggggcggt gttcgccgag atcggctcgc gcatggccga gttgagcggt tcccggctgg
     8461 ccgcgcagca acagatggaa ggcctcctgg cgccgcaccg gcccaaggag cccgcgtggt
     8521 tcctggccac cgtcggcgtc tcgcccgacc accagggcaa gggtctgggc agcgccgtcg
     8581 tgctccccgg agtggaggcg gccgagcgcg ctggggtgcc cgccttcctg gagacctccg
     8641 cgccccgcaa cctccccttc tacgagcggc tcggcttcac cgtcaccgcc gacgtcgagg
     8701 tgcccgaagg accgcgcacc tggtgcatga cccgcaagcc cggtgcctga gttcgcgtct
     8761 ggaacaatca acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg
     8821 ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt
     8881 cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg
     8941 agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc
     9001 ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc
     9061 tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc
     9121 ggctgttggg cactgacaat tccgtggtgt tgtcggggaa gctgacgtcc tttccatggc
     9181 tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg
     9241 ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc
     9301 gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcctggaa
     9361 ttaattctgc agtcgagacc tagaaaaaca tggagcaatc acaagtagca atacagcagc
     9421 taccaatgct gattgtgcct ggctagaagc acaagaggag gaggaggtgg gttttccagt
     9481 cacacctcag gtacctttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt
     9541 tttaaaagaa aagaggggac tggaagggct aattcactcc caacgaagac aagatctgct
     9601 ttttgcttgt actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta
     9661 actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg
     9721 tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg
     9781 gaaaatctct agcagtagta gttcatgtca tcttattatt cagtatttat aacttgcaaa
     9841 gaaatgaata tcagagagtg agaggccttg acattgttta aacccgctga tcagcctcga
     9901 ctgtgccttc tagttgccag ccatctgttg tttgcccctc ccccgtgcct tccttgaccc
     9961 tggaaggtgc cactcccact gtcctttcct aataaaatga ggaaattgca tcgcattgtc
    10021 tgagtaggtg tcattctatt ctggggggtg gggtggggca ggacagcaag ggggaggatt
    10081 gggaagacaa tagcaggcat gctggggatg cggtgggctc tatggcttct gaggcggaaa
    10141 gaaccagctg gggctctagg gggtatcccc acgcgccctg tagcggcgca ttaagcgcgg
    10201 cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc
    10261 ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa
    10321 atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac
    10381 ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt
    10441 tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca
    10501 accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt
    10561 taaaaaatga gctgatttaa caaaaattta acgcgaatta attctgtgga atgtgtgtca
    10621 gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct
    10681 caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca
    10741 aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc
    10801 cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt tttttattta
    10861 tgcagaggcc gaggccgcct ctgcctctga gctattccag aagtagtgag gaggcttttt
    10921 tggaggccta ggcttttgca aaaagctccc gggagcttgt atatccattt tcggatctga
    10981 tcagcacgtg atgaaaaagc ctgaactcac cgcgacgtct gtcgagaagt ttctgatcga
    11041 aaagttcgac agcgtctccg acctgatgca gctctcggag ggcgaagaat ctcgtgcttt
    11101 cagcttcgat gtaggagggc gtggatatgt cctgcgggta aatagctgcg ccgatggttt
    11161 ctacaaagat cgttatgttt atcggcactt tgcatcggcc gcgctcccga ttccggaagt
    11221 gcttgacatt ggggaattaa ttcagcgaga gcctgaccta ttgcatctcc cgccgtgcac
    11281 agggtgtcac gttgcaagac ctgcctgaaa ccgaactgcc cgctgttctg cagccggtcg
    11341 cggaggccat ggatgcgatc gctgcggccg atcttagcca gacgagcggg ttcggcccat
    11401 tcggaccgca aggaatcggt caatacacta catggcgtga tttcatatgc gcgattgctg
    11461 atccccatgt gtatcactgg caaactgtga tggacgacac cgtcagtgcg tccgtcgcgc
    11521 aggctctcga tgagctgatg ctttgggccg aggactgccc cgaagtccgg cacctcgtgc
    11581 acgcggattt cggctccaac aatgtcctga cggacaatgg ccgcataaca gcggtcattg
    11641 actggagcga ggcgatgttc ggggattccc aatacgaggt cgccaacatc ttcttctgga
    11701 ggccgtggtt ggcttgtatg gagcagcaga cgcgctactt cgagcggagg catccggagc
    11761 ttgcaggatc gccgcggctc cgggcgtata tgctccgcat tggtcttgac caactctatc
    11821 agagcttggt tgacggcaat ttcgatgatg cagcttgggc gcagggtcga tgcgacgcaa
    11881 tcgtccgatc cggagccggg actgtcgggc gtacacaaat cgcccgcaga agcgcggccg
    11941 tctggaccga tggctgtgta gaagtactcg ccgatagtgg aaaccgacgc cccagcactc
    12001 gtccgagggc aaaggaatag cacgtgctac gagatttcga ttccaccgcc gccttctatg
    12061 aaaggttggg cttcggaatc gttttccggg acgccggctg gatgatcctc cagcgcgggg
    12121 atctcatgct ggagttcttc gcccacccca acttgtttat tgcagcttat aatggttaca
    12181 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt
    12241 gtggtttgtc caaactcatc aatgtatctt atcatgtctg tataccgtcg acctctagct
    12301 agagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa
    12361 ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga
    12421 gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt
    12481 gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct
    12541 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
    12601 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
    12661 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
    12721 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
    12781 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
    12841 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
    12901 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
    12961 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
    13021 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
    13081 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
    13141 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta
    13201 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
    13261 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
    13321 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
    13381 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
    13441 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
    13501 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
    13561 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
    13621 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
    13681 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
    13741 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
    13801 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
    13861 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
    13921 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
    13981 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
    14041 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
    14101 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
    14161 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
    14221 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
    14281 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
    14341 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
    14401 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
    14461 aagtgccacc tgacgtcgac ggatcgggag atcaacttgt ttattgcagc ttataatggt
    14521 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct
    14581 agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggatcaa ctggataact
    14641 caagctaacc aaaatcatcc caaacttccc accccatacc ctattaccac tgccaattac
    14701 ctgtggtttc atttactcta aacctgtgat tcctctgaat tattttcatt ttaaagaaat
    14761 tgtatttgtt aaatatgtac tacaaactta gtagt
//
