LOCUS       sequence-164673-        5274 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing HIF1A , part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5274)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 5274)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_HIF1A_WT
FEATURES             Location/Qualifiers
     source          1..5274
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(3153..3252)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(3269..3288)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3270..3288)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3293..3309)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3293..3309)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             3740..4531
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      4624..5212
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5113..5132
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agttggcacc atggagggcg ccggcggcgc gaacgacaag aaaaagataa gttctgaacg
      721 tcgaaaagaa aagtctcgag atgcagccag atctcggcga agtaaagaat ctgaagtttt
      781 ttatgagctt gctcatcagt tgccacttcc acataatgtg agttcgcatc ttgataaggc
      841 ctctgtgatg aggcttacca tcagctattt gcgtgtgagg aaacttctgg atgctggtga
      901 tttggatatt gaagatgaca tgaaagcaca gatgaattgc ttttatttga aagccttgga
      961 tggttttgtt atggttctca cagatgatgg tgacatgatt tacatttctg ataatgtgaa
     1021 caaatacatg ggattaactc agtttgaact aactggacac agtgtgtttg attttactca
     1081 tccatgtgac catgaggaaa tgagagaaat gcttacacac agaaatggcc ttgtgaaaaa
     1141 gggtaaagaa caaaacacac agcgaagctt ttttctcaga atgaagtgta ccctaactag
     1201 ccgaggaaga actatgaaca taaagtctgc aacatggaag gtattgcact gcacaggcca
     1261 cattcacgta tatgatacca acagtaacca acctcagtgt gggtataaga aaccacctat
     1321 gacctgcttg gtgctgattt gtgaacccat tcctcaccca tcaaatattg aaattccttt
     1381 agatagcaag actttcctca gtcgacacag cctggatatg aaattttctt attgtgatga
     1441 aagaattacc gaattgatgg gatatgagcc agaagaactt ttaggccgct caatttatga
     1501 atattatcat gctttggact ctgatcatct gaccaaaact catcatgata tgtttactaa
     1561 aggacaagtc accacaggac agtacaggat gcttgccaaa agaggtggat atgtctgggt
     1621 tgaaactcaa gcaactgtca tatataacac caagaattct caaccacagt gcattgtatg
     1681 tgtgaattac gttgtgagtg gtattattca gcacgacttg attttctccc ttcaacaaac
     1741 agaatgtgtc cttaaaccgg ttgaatcttc agatatgaaa atgactcagc tattcaccaa
     1801 agttgaatca gaagatacaa gtagcctctt tgacaaactt aagaaggaac ctgatgcttt
     1861 aactttgctg gccccagccg ctggagacac aatcatatct ttagattttg gcagcaacga
     1921 cacagaaact gatgaccagc aacttgagga agtaccatta tataatgatg taatgctccc
     1981 ctcacccaac gaaaaattac agaatataaa tttggcaatg tctccattac ccaccgctga
     2041 aacgccaaag ccacttcgaa gtagtgctga ccctgcactc aatcaagaag ttgcattaaa
     2101 attagaacca aatccagagt cactggaact ttcttttacc atgccccaga ttcaggatca
     2161 gacacctagt ccttccgatg gaagcactag acaaagttca cctgagccta atagtcccag
     2221 tgaatattgt ttttatgtgg atagtgatat ggtcaatgaa ttcaagttgg aattggtaga
     2281 aaaacttttt gctgaagaca cagaagcaaa gaacccattt tctactcagg acacagattt
     2341 agacttggag atgttagctc cctatatccc aatggatgat gacttccagt tacgttcctt
     2401 cgatcagttg tcaccattag aaagcagttc cgcaagccct gaaagcgcaa gtcctcaaag
     2461 cacagttaca gtattccagc agactcaaat acaagaacct actgctaatg ccaccactac
     2521 cactgccacc actgatgaat taaaaacagt gacaaaagac cgtatggaag acattaaaat
     2581 attgattgca tctccatctc ctacccacat acataaagaa actactagtg ccacatcatc
     2641 accatataga gatactcaaa gtcggacagc ctcaccaaac agagcaggaa aaggagtcat
     2701 agaacagaca gaaaaatctc atccaagaag ccctaacgtg ttatctgtcg ctttgagtca
     2761 aagaactaca gttcctgagg aagaactaaa tccaaagata ctagctttgc agaatgctca
     2821 gagaaagcga aaaatggaac atgatggttc actttttcaa gcagtaggaa ttggaacatt
     2881 attacagcag ccagacgatc atgcagctac tacatcactt tcttggaaac gtgtaaaagg
     2941 atgcaaatct agtgaacaga atggaatgga gcaaaagaca attattttaa taccctctga
     3001 tttagcatgt agactgctgg ggcaatcaat ggatgaaagt ggattaccac agctgaccag
     3061 ttatgattgt gaagttaatg ctcctataca aggcagcaga aacctactgc agggtgaaga
     3121 attactcaga gctttggatc aagttaactg attgccaact ttcttgtaca aagttggcat
     3181 tataagaaag cattgcttat caatttgttg caacgaacag gtcactatca gtcaaaataa
     3241 aatcattatt tgccatccag ctgatatccc ctatagtgag tcgtattaca tggtcatagc
     3301 tgtttcctgg cagctctggc ccgtgtctca aaatctctga tgttacattg cacaagataa
     3361 aaatatatca tcatgcctcc tctagaccag ccaggacaga aatgcctcga cttcgctgct
     3421 acccaaggtt gccgggtgac gcacaccgtg gaaacggatg aaggcacgaa cccagtggac
     3481 ataagcctgt tcggttcgta agctgtaatg caagtagcgt atgcgctcac gcaactggtc
     3541 cagaaccttg accgaacgca gcggtggtaa cggcgcagtg gcggttttca tggcttgtta
     3601 tgactgtttt tttggggtac agtctatgcc tcgggcatcc aagcagcaag cgcgttacgc
     3661 cgtgggtcga tgtttgatgt tatggagcag caacgatgtt acgcagcagg gcagtcgccc
     3721 taaaacaaag ttaaacatta tgagggaagc ggtgatcgcc gaagtatcga ctcaactatc
     3781 agaggtagtt ggcgtcatcg agcgccatct cgaaccgacg ttgctggccg tacatttgta
     3841 cggctccgca gtggatggcg gcctgaagcc acacagtgat attgatttgc tggttacggt
     3901 gaccgtaagg cttgatgaaa caacgcggcg agctttgatc aacgaccttt tggaaacttc
     3961 ggcttcccct ggagagagcg agattctccg cgctgtagaa gtcaccattg ttgtgcacga
     4021 cgacatcatt ccgtggcgtt atccagctaa gcgcgaactg caatttggag aatggcagcg
     4081 caatgacatt cttgcaggta tcttcgagcc agccacgatc gacattgatc tggctatctt
     4141 gctgacaaaa gcaagagaac atagcgttgc cttggtaggt ccagcggcgg aggaactctt
     4201 tgatccggtt cctgaacagg atctatttga ggcgctaaat gaaaccttaa cgctatggaa
     4261 ctcgccgccc gactgggctg gcgatgagcg aaatgtagtg cttacgttgt cccgcatttg
     4321 gtacagcgca gtaaccggca aaatcgcgcc gaaggatgtc gctgccgact gggcaatgga
     4381 gcgcctgccg gcccagtatc agcccgtcat acttgaagct agacaggctt atcttggaca
     4441 agaagaagat cgcttggcct cgcgcgcaga tcagttggaa gaatttgtcc actacgtgaa
     4501 aggcgagatc accaaggtag tcggcaaata accctcgagc cacccatgac caaaatccct
     4561 taacgtgagt tacgcgtcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
     4621 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
     4681 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
     4741 cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca
     4801 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
     4861 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
     4921 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
     4981 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
     5041 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
     5101 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
     5161 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
     5221 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgtt
//
