LOCUS       sequence-164675-        5205 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing HSP90B1 , part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5205)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 5205)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_HSP90B1_WT
FEATURES             Location/Qualifiers
     source          1..5205
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(3084..3183)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(3200..3219)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3201..3219)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3224..3240)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3224..3240)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             3671..4462
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      4555..5143
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5044..5063
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agttggcacc atgagggccc tgtgggtgct gggcctctgc tgcgtcctgc tgaccttcgg
      721 gtcggtcaga gctgacgatg aagttgatgt ggatggtaca gtagaagagg atctgggtaa
      781 aagtagagaa ggatcaagga cggatgatga agtagtacag agagaggaag aagctattca
      841 gttggatgga ttaaatgcat cacaaataag agaacttaga gagaagtcgg aaaagtttgc
      901 cttccaagcc gaagttaaca gaatgatgaa acttatcatc aattcattgt ataaaaataa
      961 agagattttc ctgagagaac tgatttcaaa tgcttctgat gctttagata agataaggct
     1021 aatatcactg actgatgaaa atgctctttc tggaaatgag gaactaacag tcaaaattaa
     1081 gtgtgataag gagaagaacc tgctgcatgt cacagacacc ggtgtaggaa tgaccagaga
     1141 agagttggtt aaaaaccttg gtaccatagc caaatctggg acaagcgagt ttttaaacaa
     1201 aatgactgaa gcacaggaag atggccagtc aacttctgaa ttgattggcc agtttggtgt
     1261 cggtttctat tccgccttcc ttgtagcaga taaggttatt gtcacttcaa aacacaacaa
     1321 cgatacccag cacatctggg agtctgactc caatgaattt tctgtaattg ctgacccaag
     1381 aggaaacact ctaggacggg gaacgacaat tacccttgtc ttaaaagaag aagcatctga
     1441 ttaccttgaa ttggatacaa ttaaaaatct cgtcaaaaaa tattcacagt tcataaactt
     1501 tcctatttat gtatggagca gcaagactga aactgttgag gagcccatgg aggaagaaga
     1561 agcagccaaa gaagagaaag aagaatctga tgatgaagct gcagtagagg aagaagaaga
     1621 agaaaagaaa ccaaagacta aaaaagttga aaaaactgtc tgggactggg aacttatgaa
     1681 tgatatcaaa ccaatatggc agagaccatc aaaagaagta gaagaagatg aatacaaagc
     1741 tttctacaaa tcattttcaa aggaaagtga tgaccccatg gcttatattc actttactgc
     1801 tgaaggggaa gttaccttca aatcaatttt atttgtaccc acatctgctc cacgtggtct
     1861 gtttgacgaa tatggatcta aaaagagcga ttacattaag ctctatgtgc gccgtgtatt
     1921 catcacagac gacttccatg atatgatgcc taaatacctc aattttgtca agggtgtggt
     1981 ggactcagat gatctcccct tgaatgtttc ccgcgagact cttcagcaac ataaactgct
     2041 taaggtgatt aggaagaagc ttgttcgtaa aacgctggac atgatcaaga agattgctga
     2101 tgataaatac aatgatactt tttggaaaga atttggtacc aacatcaagc ttggtgtgat
     2161 tgaagaccac tcgaatcgaa cacgtcttgc taaacttctt aggttccagt cttctcatca
     2221 tccaactgac attactagcc tagaccagta tgtggaaaga atgaaggaaa aacaagacaa
     2281 aatctacttc atggctgggt ccagcagaaa agaggctgaa tcttctccat ttgttgagcg
     2341 acttctgaaa aagggctatg aagttattta cctcacagaa cctgtggatg aatactgtat
     2401 tcaggccctt cccgaatttg atgggaagag gttccagaat gttgccaagg aaggagtgaa
     2461 gttcgatgaa agtgagaaaa ctaaggagag tcgtgaagca gttgagaaag aatttgagcc
     2521 tctgctgaat tggatgaaag ataaagccct taaggacaag attgaaaagg ctgtggtgtc
     2581 tcagcgcctg acagaatctc cgtgtgcttt ggtggccagc cagtacggat ggtctggcaa
     2641 catggagaga atcatgaaag cacaagcgta ccaaacgggc aaggacatct ctacaaatta
     2701 ctatgcgagt cagaagaaaa catttgaaat taatcccaga cacccgctga tcagagacat
     2761 gcttcgacga attaaggaag atgaagatga taaaacagtt ttggatcttg ctgtggtttt
     2821 gtttgaaaca gcaacgcttc ggtcagggta tcttttacca gacactaaag catatggaga
     2881 tagaatagaa agaatgcttc gcctcagttt gaacattgac cctgatgcaa aggtggaaga
     2941 agagcctgaa gaagaacctg aagagacagc agaagacaca acagaagaca cagagcaaga
     3001 cgaagatgaa gaaatggatg tgggaacaga tgaagaagaa gaaacagcaa aggaatctac
     3061 agctgaaaaa gatgaattgt aattgccaac tttcttgtac aaagttggca ttataagaaa
     3121 gcattgctta tcaatttgtt gcaacgaaca ggtcactatc agtcaaaata aaatcattat
     3181 ttgccatcca gctgatatcc cctatagtga gtcgtattac atggtcatag ctgtttcctg
     3241 gcagctctgg cccgtgtctc aaaatctctg atgttacatt gcacaagata aaaatatatc
     3301 atcatgcctc ctctagacca gccaggacag aaatgcctcg acttcgctgc tacccaaggt
     3361 tgccgggtga cgcacaccgt ggaaacggat gaaggcacga acccagtgga cataagcctg
     3421 ttcggttcgt aagctgtaat gcaagtagcg tatgcgctca cgcaactggt ccagaacctt
     3481 gaccgaacgc agcggtggta acggcgcagt ggcggttttc atggcttgtt atgactgttt
     3541 ttttggggta cagtctatgc ctcgggcatc caagcagcaa gcgcgttacg ccgtgggtcg
     3601 atgtttgatg ttatggagca gcaacgatgt tacgcagcag ggcagtcgcc ctaaaacaaa
     3661 gttaaacatt atgagggaag cggtgatcgc cgaagtatcg actcaactat cagaggtagt
     3721 tggcgtcatc gagcgccatc tcgaaccgac gttgctggcc gtacatttgt acggctccgc
     3781 agtggatggc ggcctgaagc cacacagtga tattgatttg ctggttacgg tgaccgtaag
     3841 gcttgatgaa acaacgcggc gagctttgat caacgacctt ttggaaactt cggcttcccc
     3901 tggagagagc gagattctcc gcgctgtaga agtcaccatt gttgtgcacg acgacatcat
     3961 tccgtggcgt tatccagcta agcgcgaact gcaatttgga gaatggcagc gcaatgacat
     4021 tcttgcaggt atcttcgagc cagccacgat cgacattgat ctggctatct tgctgacaaa
     4081 agcaagagaa catagcgttg ccttggtagg tccagcggcg gaggaactct ttgatccggt
     4141 tcctgaacag gatctatttg aggcgctaaa tgaaacctta acgctatgga actcgccgcc
     4201 cgactgggct ggcgatgagc gaaatgtagt gcttacgttg tcccgcattt ggtacagcgc
     4261 agtaaccggc aaaatcgcgc cgaaggatgt cgctgccgac tgggcaatgg agcgcctgcc
     4321 ggcccagtat cagcccgtca tacttgaagc tagacaggct tatcttggac aagaagaaga
     4381 tcgcttggcc tcgcgcgcag atcagttgga agaatttgtc cactacgtga aaggcgagat
     4441 caccaaggta gtcggcaaat aaccctcgag ccacccatga ccaaaatccc ttaacgtgag
     4501 ttacgcgtcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
     4561 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
     4621 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
     4681 agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa
     4741 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
     4801 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
     4861 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
     4921 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
     4981 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
     5041 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
     5101 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
     5161 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgtt
//
