LOCUS       sequence-164678-        4758 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing HSPA5 , part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4758)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 4758)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_HSPA5_WT
FEATURES             Location/Qualifiers
     source          1..4758
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2637..2736)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2753..2772)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2754..2772)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2777..2793)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2777..2793)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             3224..4015
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      4108..4696
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4597..4616
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agttggcacc atgaagctct ccctggtggc cgcgatgctg ctgctgctca gcgcggcgcg
      721 ggccgaggag gaggacaaga aggaggacgt gggcacggtg gtcggcatcg acctggggac
      781 cacctactcc tgcgtcggcg tgttcaagaa cggccgcgtg gagatcatcg ccaacgatca
      841 gggcaaccgc atcacgccgt cctatgtcgc cttcactcct gaaggggaac gtctgattgg
      901 cgatgccgcc aagaaccagc tcacctccaa ccccgagaac acggtctttg acgccaagcg
      961 gctcatcggc cgcacgtgga atgacccgtc tgtgcagcag gacatcaagt tcttgccgtt
     1021 caaggtggtt gaaaagaaaa ctaaaccata cattcaagtt gatattggag gtgggcaaac
     1081 aaagacattt gctcctgaag aaatttctgc catggttctc actaaaatga aagaaaccgc
     1141 tgaggcttat ttgggaaaga aggttaccca tgcagttgtt actgtaccag cctattttaa
     1201 tgatgcccaa cgccaagcaa ccaaagacgc tggaactatt gctggcctaa atgttatgag
     1261 gatcatcaac gagcctacgg cagctgctat tgcttatggc ctggataaga gggaggggga
     1321 gaagaacatc ctggtgtttg acctgggtgg cggaaccttc gatgtgtctc ttctcaccat
     1381 tgacaatggt gtcttcgaag ttgtggccac taatggagat actcatctgg gtggagaaga
     1441 ctttgaccag cgtgtcatgg aacacttcat caaactgtac aaaaagaaga cgggcaaaga
     1501 tgtcaggaaa gacaatagag ctgtgcagaa actccggcgc gaggtagaaa aggccaaacg
     1561 ggccctgtct tctcagcatc aagcaagaat tgaaattgag tccttctatg aaggagaaga
     1621 cttttctgag accctgactc gggccaaatt tgaagagctc aacatggatc tgttccggtc
     1681 tactatgaag cccgtccaga aagtgttgga agattctgat ttgaagaagt ctgatattga
     1741 tgaaattgtt cttgttggtg gctcgactcg aattccaaag attcagcaac tggttaaaga
     1801 gttcttcaat ggcaaggaac catcccgtgg cataaaccca gatgaagctg tagcgtatgg
     1861 tgctgctgtc caggctggtg tgctctctgg tgatcaagat acaggtgacc tggtactgct
     1921 tgatgtatgt ccccttacac ttggtattga aactgtggga ggtgtcatga ccaaactgat
     1981 tccaaggaac acagtggtgc ctaccaagaa gtctcagatc ttttctacag cttctgataa
     2041 tcaaccaact gttacaatca aggtctatga aggtgaaaga cccctgacaa aagacaatca
     2101 tcttctgggt acatttgatc tgactggaat tcctcctgct cctcgtgggg tcccacagat
     2161 tgaagtcacc tttgagatag atgtgaatgg tattcttcga gtgacagctg aagacaaggg
     2221 tacagggaac aaaaataaga tcacaatcac caatgaccag aatcgcctga cacctgaaga
     2281 aatcgaaagg atggttaatg atgctgagaa gtttgctgag gaagacaaaa agctcaagga
     2341 gcgcattgat actagaaatg agttggaaag ctatgcctat tctctaaaga atcagattgg
     2401 agataaagaa aagctgggag gtaaactttc ctctgaagat aaggagacca tggaaaaagc
     2461 tgtagaagaa aagattgaat ggctggaaag ccaccaagat gctgacattg aagacttcaa
     2521 agctaagaag aaggaactgg aagaaattgt tcaaccaatt atcagcaaac tctatggaag
     2581 tgcaggccct cccccaactg gtgaagagga tacagcagaa aaagatgagt tgtagttgcc
     2641 aactttcttg tacaaagttg gcattataag aaagcattgc ttatcaattt gttgcaacga
     2701 acaggtcact atcagtcaaa ataaaatcat tatttgccat ccagctgata tcccctatag
     2761 tgagtcgtat tacatggtca tagctgtttc ctggcagctc tggcccgtgt ctcaaaatct
     2821 ctgatgttac attgcacaag ataaaaatat atcatcatgc ctcctctaga ccagccagga
     2881 cagaaatgcc tcgacttcgc tgctacccaa ggttgccggg tgacgcacac cgtggaaacg
     2941 gatgaaggca cgaacccagt ggacataagc ctgttcggtt cgtaagctgt aatgcaagta
     3001 gcgtatgcgc tcacgcaact ggtccagaac cttgaccgaa cgcagcggtg gtaacggcgc
     3061 agtggcggtt ttcatggctt gttatgactg tttttttggg gtacagtcta tgcctcgggc
     3121 atccaagcag caagcgcgtt acgccgtggg tcgatgtttg atgttatgga gcagcaacga
     3181 tgttacgcag cagggcagtc gccctaaaac aaagttaaac attatgaggg aagcggtgat
     3241 cgccgaagta tcgactcaac tatcagaggt agttggcgtc atcgagcgcc atctcgaacc
     3301 gacgttgctg gccgtacatt tgtacggctc cgcagtggat ggcggcctga agccacacag
     3361 tgatattgat ttgctggtta cggtgaccgt aaggcttgat gaaacaacgc ggcgagcttt
     3421 gatcaacgac cttttggaaa cttcggcttc ccctggagag agcgagattc tccgcgctgt
     3481 agaagtcacc attgttgtgc acgacgacat cattccgtgg cgttatccag ctaagcgcga
     3541 actgcaattt ggagaatggc agcgcaatga cattcttgca ggtatcttcg agccagccac
     3601 gatcgacatt gatctggcta tcttgctgac aaaagcaaga gaacatagcg ttgccttggt
     3661 aggtccagcg gcggaggaac tctttgatcc ggttcctgaa caggatctat ttgaggcgct
     3721 aaatgaaacc ttaacgctat ggaactcgcc gcccgactgg gctggcgatg agcgaaatgt
     3781 agtgcttacg ttgtcccgca tttggtacag cgcagtaacc ggcaaaatcg cgccgaagga
     3841 tgtcgctgcc gactgggcaa tggagcgcct gccggcccag tatcagcccg tcatacttga
     3901 agctagacag gcttatcttg gacaagaaga agatcgcttg gcctcgcgcg cagatcagtt
     3961 ggaagaattt gtccactacg tgaaaggcga gatcaccaag gtagtcggca aataaccctc
     4021 gagccaccca tgaccaaaat cccttaacgt gagttacgcg tcgttccact gagcgtcaga
     4081 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
     4141 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
     4201 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct
     4261 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     4321 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
     4381 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
     4441 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
     4501 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
     4561 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
     4621 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
     4681 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
     4741 gccttttgct cacatgtt
//
