LOCUS       sequence-16468-e         882 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 882)
  TITLE     pBS-p85alpha
REFERENCE   2  (bases 1 to 882)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: Myr-(iSH2-p85)-p110alpha-Myc
FEATURES             Location/Qualifiers
     source          1..882
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             184..213
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     polyA_signal    221..355
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(245..264)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     299..318
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(498..520)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     661..680
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(794..811)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 ccagaactac aatcttttga tgacattgca tatatccgaa agactctagc cttggacaaa
       61 actgagcaag aagctttgga atatttcaca aagcaaatga atgatgcaca tcatggtgga
      121 tggacgacaa aaatggattg gatcttccac accatcaagc agcatgcttt gaacgagctc
      181 ggggagcaga agctgatttc cgaggaggac ctgaactgag gatccagaca tgataagata
      241 cattgatgag tttggacaaa ccacaactag aatgcagtga aaaaaatgct ttatttgtga
      301 aatttgtgat gctattgctt tatttgtaac cattataagc tgcaataaac aagttaacaa
      361 caacaattgc attcatttta tgtttcaggt tcagggggag gtgtgggagg ttttttaaag
      421 caagtaaaac ctctacaaat gtggtatggc tgattatgat ccggctgcct cgcgcgtttc
      481 ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg agacggtcac agcttgtctg
      541 taagcggatg ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt
      601 cggggcgcag ccatgaccca gtcacgtagc gatagcggag tgtatactgg cttaactatg
      661 cggcatcaga gcagattgta ctgagagtgc accataggtc gaccggtcga ccggtcgacc
      721 ggtcgaccta tgcggtgtga aataccgcac agatgcgtaa ggagaaaata ccgcatcagc
      781 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg
      841 tatcagctca ctcaaaggcg gtaatacggt tatccacaga tc
//
