LOCUS       sequence-164687-         816 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses ecliptic pHluorin–ICAM-1 (intercellular adhesion molecule 
            1) in mammalian cells. Incorporates into viral membrane to label 
            pseudoviruses.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 816)
  AUTHORS   Miyauchi K, Marin M, Melikyan GB
  TITLE     Visualization of retrovirus uptake and delivery into acidic 
            endosomes.
  JOURNAL   Biochem J. 2011 Mar 15;434(3):559-69. doi: 10.1042/BJ20101588.
  PUBMED    21175427
REFERENCE   2  (bases 1 to 816)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Biochem J"; date: "2011-03-15- 15"; volume: "434"; issue: "3"; 
            pages: "559-69"
COMMENT     Sequence Label: EcpH-TM
FEATURES             Location/Qualifiers
     source          1..816
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1..711
                     /codon_start=1
                     /product="pH-sensitive mutant of green fluorescent protein 
                     (Ng et al., 2002)"
                     /label=superecliptic pHluorin
                     /note="excited strongly by blue light at neutral pH but 
                     minimally fluorescent at acidic pH"
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF
                     ICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNDHQVYIMADKQKNGIKAN
                     FKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLFTTSTLSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
     primer_bind     complement(28..56)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     651..671
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
ORIGIN
        1 agtaaaggag aagaactttt cactggagtt gtcccaattc ttgttgaatt agatggtgat
       61 gttaatgggc acaaattttc tgtcagtgga gagggtgaag gtgatgcaac atacggaaaa
      121 cttaccctta aatttatttg cactactgga aaactacctg ttccttggcc aacacttgtc
      181 actactttaa cttatggtgt tcaatgcttt tcaagatacc cagatcatat gaaacggcat
      241 gactttttca agagtgccat gcccgaaggt tatgtacagg aaagaactat atttttcaaa
      301 gatgacggga actacaagac acgtgctgaa gtcaagtttg aaggtgatac ccttgttaat
      361 agaatcgagt taaaaggtat tgattttaaa gaagatggaa acattcttgg acacaaattg
      421 gaatacaact ataacgatca ccaggtgtac atcatggcag acaaacaaaa gaatggaatc
      481 aaagctaact tcaaaattag acacaacatt gaagatggag gcgttcaact agcagaccat
      541 tatcaacaaa atactccaat tggcgatggg cccgtccttt taccagacaa ccattacctg
      601 tttacaactt ctactctttc gaaagatccc aacgaaaaga gagaccacat ggtccttctt
      661 gagtttgtaa cagctgctgg gattacacat ggcatggatg aactatacaa gaattcacgg
      721 tatgagattg tcatcatcac tgtggtagca gccgcagtca taatgggcac tgcaggcctc
      781 agcacgtacc tctataaccg ccagcggtga ggatcc
//
