LOCUS       sequence-164700-         932 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Human PC1-CTp30 expression cassette with a patient mutation at R4150
            and a GST-tag (850).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 932)
  AUTHORS   Doerr N, Wang Y, Kipp KR, Liu G, Benza JJ, Pletnev V, Pavlov TS, 
            Staruschenko A, Mohieldin AM, Takahashi M, Nauli SM, Weimbs T
  TITLE     Regulation of Polycystin-1 Function by Calmodulin Binding.
  JOURNAL   PLoS One. 2016 Aug 25;11(8):e0161525. doi: 
            10.1371/journal.pone.0161525. eCollection 2016.
  PUBMED    27560828
REFERENCE   2  (bases 1 to 932)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2016-08-25"; volume: "11(8):e0161525. doi"; pages: " 
            10.1371/journal.pone.0161525. eCollection 2016"
COMMENT     Sequence Label: pcDNA4/TO h-PKD1 FLS R4150C-GST
FEATURES             Location/Qualifiers
     source          1..932
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    20..38
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     48..72
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
ORIGIN
        1 cctatcagtg atagagatct ccctatcagt gatagagatc gtcgacgagc tcgtttagtg
       61 aaccgtcaga tcgcctggag acgccatcca cgctgttttg acctccatag aagacaccgg
      121 gaccgatcca gcctccggac tctagcgttt aaacttaagc ttggtaccga gctcggatcc
      181 actagtccag tgtggtggaa ttctgttatg gcccgctggc gctaccacgc tttgcgtgga
      241 gagctgtacc ggccggcctg ggagccccag gactacgaga tggtggagtt gttcctgcgc
      301 aggctgcgcc tctggatggg cctcagcaag gtcaaggagt tctgccacaa agtccgcttt
      361 gaagggatgg agccgctgcc ctctcgctcc tccaggggct ccaaggtatc cccggatgtg
      421 cccccaccca gcgctggctc cgatgcctcg cacccctcca cctcctccag ccagctggat
      481 gggctgagcg tgagcctggg ccggctgggg acaaggtgtg agcccgagcc ctcccgcctc
      541 caagccgtgt tcgaggccct gctcacccag tttgaccgac tcaaccaggc cacagaggac
      601 gtctaccagc tggagcagca gctgcacagc ctgcaaggcc gcaggagcag ccgggcgccc
      661 gccggatctt cccgtggccc atccccgggc ctgcggccag cactgcccag ccgccttgcc
      721 cgggccagtc ggggtgtgga cctggccact ggccccagca ggacacccct tcgggccaag
      781 aacaaggtcc accccagcag cactgcggcc gctatgtccc ctatactagg ttattggaaa
      841 attaagggcc ttgtgcaacc cactcgactt cttttggaat atcttgaaga aaaatatgaa
      901 gagcatttgt atgagcgcga tgaaggtgat aa
//
