LOCUS       sequence-164701-         943 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Human PC1-CTp30 expression cassette with a patient mutation at R4150
            and a GST-tag (850).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 943)
  AUTHORS   Doerr N, Wang Y, Kipp KR, Liu G, Benza JJ, Pletnev V, Pavlov TS, 
            Staruschenko A, Mohieldin AM, Takahashi M, Nauli SM, Weimbs T
  TITLE     Regulation of Polycystin-1 Function by Calmodulin Binding.
  JOURNAL   PLoS One. 2016 Aug 25;11(8):e0161525. doi: 
            10.1371/journal.pone.0161525. eCollection 2016.
  PUBMED    27560828
REFERENCE   2  (bases 1 to 943)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2016-08-25"; volume: "11(8):e0161525. doi"; pages: " 
            10.1371/journal.pone.0161525. eCollection 2016"
COMMENT     Sequence Label: pcDNA4/TO h-PKD1 FLS R4150C-GST
FEATURES             Location/Qualifiers
     source          1..943
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(26..55)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             complement(82..735)
                     /codon_start=1
                     /product="glutathione S-transferase from Schistosoma 
                     japonicum"
                     /label=GST
                     /translation="MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKK
                     FELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRY
                     GVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVL
                     YMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK"
     primer_bind     complement(102..124)
                     /label=pGEX 5'
                     /note="pGEX vectors, Glutathione-S-transferase, forward 
                     primer"
ORIGIN
        1 ggtgatgatg accggtatgc atattcagat cctcttctga gatgagtttt tgttcgaatg
       61 ggtgacctcg agctaatccg attttggagg atggtcgcca ccaccaaacg tggcttgcca
      121 gccctgcaaa ggccatgcta tatacttgct ggatttcaag tacttatcaa tttgtgggat
      181 agcttcaata cgttttttaa aacaaactaa ttttgggaac gcatccaggc acattgggtc
      241 catgtataaa acaacatcaa gagcgtcata caacatgaag tcaggatggg ttacatgatc
      301 accatttaaa tatgttttat gacataaacg atcttcgaac attttcagca tttcaggtag
      361 cttgctaaga aaatcaactt tgagagtttc aaagtcttta ctatatgcaa ttctcgaaac
      421 accgtatcta atatccaaaa ccgctccttc aagcattgaa atctctgcac gctcttttgg
      481 acaaccaccc aacatgttgt gcttgtcagc tatataacgt atgatggcca tagactgtgt
      541 taatttaaca tcaccatcaa tataataagg aagattggga aactccaaac ccaattcaaa
      601 ctttttgttt cgccatttat caccttcatc gcgctcatac aaatgctctt catatttttc
      661 ttcaagatat tccaaaagaa gtcgagtggg ttgcacaagg cccttaattt tccaataacc
      721 tagtataggg gacatagcgg ccgcagtgct gctggggtgg accttgttct tggcccgaag
      781 gggtgtcctg ctggggccag tggccaggtc cacaccccga ctggcccggg caaggcggct
      841 gggcagtgct ggccgcaggc ccggggatgg gccacgggaa gatccggcgg gcgcccggct
      901 gctcctgcgg ccttgcaggc tgtgcagctg ctgctccagc tgg
//
