LOCUS       sequence-164706-         981 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Human PC1-CTp30 expression cassette with a patient mutation at L4137
            and a GST-tag (848).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 981)
  AUTHORS   Doerr N, Wang Y, Kipp KR, Liu G, Benza JJ, Pletnev V, Pavlov TS, 
            Staruschenko A, Mohieldin AM, Takahashi M, Nauli SM, Weimbs T
  TITLE     Regulation of Polycystin-1 Function by Calmodulin Binding.
  JOURNAL   PLoS One. 2016 Aug 25;11(8):e0161525. doi: 
            10.1371/journal.pone.0161525. eCollection 2016.
  PUBMED    27560828
REFERENCE   2  (bases 1 to 981)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2016-08-25"; volume: "11(8):e0161525. doi"; pages: " 
            10.1371/journal.pone.0161525. eCollection 2016"
COMMENT     Sequence Label: pcDNA4/TO h-PKD1 FLS L4137P-GST
FEATURES             Location/Qualifiers
     source          1..981
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(30..59)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             complement(86..739)
                     /codon_start=1
                     /product="glutathione S-transferase from Schistosoma 
                     japonicum"
                     /label=GST
                     /translation="MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKK
                     FELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRY
                     GVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVL
                     YMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK"
     primer_bind     complement(106..128)
                     /label=pGEX 5'
                     /note="pGEX vectors, Glutathione-S-transferase, forward 
                     primer"
ORIGIN
        1 tgatggtgat gatgaccggt atgcatattc agatcctctt ctgagatgag tttttgttcg
       61 aatgggtgac ctcgagctaa tccgattttg gaggatggtc gccaccacca aacgtggctt
      121 gccagccctg caaaggccat gctatatact tgctggattt caagtactta tcaatttgtg
      181 ggatagcttc aatacgtttt ttaaaacaaa ctaattttgg gaacgcatcc aggcacattg
      241 ggtccatgta taaaacaaca tcaagagcgt catacaacat gaagtcagga tgggttacat
      301 gatcaccatt taaatatgtt ttatgacata aacgatcttc gaacattttc agcatttcag
      361 gtagcttgct aagaaaatca actttgagag tttcaaagtc tttactatat gcaattctcg
      421 aaacaccgta tctaatatcc aaaaccgctc cttcaagcat tgaaatctct gcacgctctt
      481 ttggacaacc acccaacatg ttgtgcttgt cagctatata acgtatgatg gccatagact
      541 gtgttaattt aacatcacca tcaatataat aaggaagatt gggaaactcc aaacccaatt
      601 caaacttttt gtttcgccat ttatcacctt catcgcgctc atacaaatgc tcttcatatt
      661 tttcttcaag atattccaaa agaagtcgag tgggttgcac aaggccctta attttccaat
      721 aacctagtat aggggacata gcggccgcag tgctgctggg gtggaccttg ttcttggccc
      781 gaaggggtgt cctgctgggg ccagtggcca ggtccacacc ccgactggcc cgggcaaggc
      841 ggctgggcag tgctggccgc aggcccgggg atgggccacg ggaagatccg gcgggcgccc
      901 ggctgctcct gcggccttgc aggctgtgca gctgctgctc cagctggtag acgtcctctg
      961 tggcctggtt gagtcggtca a
//
