LOCUS       sequence-164711-         913 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Human PC1-CTp30 expression cassette with a GST-tag (678).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 913)
  AUTHORS   Doerr N, Wang Y, Kipp KR, Liu G, Benza JJ, Pletnev V, Pavlov TS, 
            Staruschenko A, Mohieldin AM, Takahashi M, Nauli SM, Weimbs T
  TITLE     Regulation of Polycystin-1 Function by Calmodulin Binding.
  JOURNAL   PLoS One. 2016 Aug 25;11(8):e0161525. doi: 
            10.1371/journal.pone.0161525. eCollection 2016.
  PUBMED    27560828
REFERENCE   2  (bases 1 to 913)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2016-08-25"; volume: "11(8):e0161525. doi"; pages: " 
            10.1371/journal.pone.0161525. eCollection 2016"
COMMENT     Sequence Label: pcDNA4/TO h-PKD1 FLS (4107-4303)-GST
FEATURES             Location/Qualifiers
     source          1..913
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(29..58)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             complement(85..738)
                     /codon_start=1
                     /product="glutathione S-transferase from Schistosoma 
                     japonicum"
                     /label=GST
                     /translation="MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKK
                     FELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRY
                     GVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVL
                     YMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK"
     primer_bind     complement(105..127)
                     /label=pGEX 5'
                     /note="pGEX vectors, Glutathione-S-transferase, forward 
                     primer"
ORIGIN
        1 gatggtgatg atgaccggta tgcatattca gatcctcttc tgagatgagt ttttgttcga
       61 atgggtgacc tcgagctaat ccgattttgg aggatggtcg ccaccaccaa acgtggcttg
      121 ccagccctgc aaaggccatg ctatatactt gctggatttc aagtacttat caatttgtgg
      181 gatagcttca atacgttttt taaaacaaac taattttggg aacgcatcca ggcacattgg
      241 gtccatgtat aaaacaacat caagagcgtc atacaacatg aagtcaggat gggttacatg
      301 atcaccattt aaatatgttt tatgacataa acgatcttcg aacattttca gcatttcagg
      361 tagcttgcta agaaaatcaa ctttgagagt ttcaaagtct ttactatatg caattctcga
      421 aacaccgtat ctaatatcca aaaccgctcc ttcaagcatt gaaatctctg cacgctcttt
      481 tggacaacca cccaacatgt tgtgcttgtc agctatataa cgtatgatgg ccatagactg
      541 tgttaattta acatcaccat caatataata aggaagattg ggaaactcca aacccaattc
      601 aaactttttg tttcgccatt tatcaccttc atcgcgctca tacaaatgct cttcatattt
      661 ttcttcaaga tattccaaaa gaagtcgagt gggttgcaca aggcccttaa ttttccaata
      721 acctagtata ggggacatag cggccgcagt gctgctgggg tggaccttgt tcttggcccg
      781 aaggggtgtc ctgctggggc cagtggccag gtccacaccc cgactggccc gggcaaggcg
      841 gctgggcagt gctggccgca ggcccgggga tgggccacgg gaagatccgg cgggcgcccg
      901 gctgctcctg cgg
//
