LOCUS       sequence-164713-         969 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Human PC1-CTp30 expression cassette lacking a Calmodulin binding 
            domain (delta C1) (632).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 969)
  AUTHORS   Doerr N, Wang Y, Kipp KR, Liu G, Benza JJ, Pletnev V, Pavlov TS, 
            Staruschenko A, Mohieldin AM, Takahashi M, Nauli SM, Weimbs T
  TITLE     Regulation of Polycystin-1 Function by Calmodulin Binding.
  JOURNAL   PLoS One. 2016 Aug 25;11(8):e0161525. doi: 
            10.1371/journal.pone.0161525. eCollection 2016.
  PUBMED    27560828
REFERENCE   2  (bases 1 to 969)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2016-08-25"; volume: "11(8):e0161525. doi"; pages: " 
            10.1371/journal.pone.0161525. eCollection 2016"
COMMENT     Sequence Label: pcDNA4/TO h-PKD1 FLS (4127-4303)-Myc/His
FEATURES             Location/Qualifiers
     source          1..969
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    20..38
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     48..72
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             762..791
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             807..824
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(847..864)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    853..964
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 cctatcagtg atagagatct ccctatcagt gatagagatc gtcgacgagc tcgtttagtg
       61 aaccgtcaga tcgcctggag acgccatcca cgctgttttg acctccatag aagacaccgg
      121 gaccgatcca gcctccggac tctagcgttt aaacttaagc ttggtaccga gctcggatcc
      181 actagtccag tgtggtggaa ttcatgtacg agatggtgga gttgttcctg cgcaggctgc
      241 gcctctggat gggcctcagc aaggtcaagg agttccgcca caaagtccgc tttgaaggga
      301 tggagccgct gccctctcgc tcctccaggg gctccaaggt atccccggat gtgcccccac
      361 ccagcgctgg ctccgatgcc tcgcacccct ccacctcctc cagccagctg gatgggctga
      421 gcgtgagcct gggccggctg gggacaaggt gtgagcccga gccctcccgc ctccaagccg
      481 tgttcgaggc cctgctcacc cagtttgacc gactcaacca ggccacagag gacgtctacc
      541 agctggagca gcagctgcac agcctgcaag gccgcaggag cagccgggcg cccgccggat
      601 cttcccgtgg cccatccccg ggcctgcggc cagcactgcc cagccgcctt gcccgggcca
      661 gtcggggtgt ggacctggcc actggcccca gcaggacacc ccttcgggcc aagaacaagg
      721 tccaccccag cagcactgcg gccgctcgag gtcacccatt cgaacaaaaa ctcatctcag
      781 aagaggatct gaatatgcat accggtcatc atcaccatca ccattgagtt taaacccgct
      841 gatcagcctc gactgtgcct tctagttgcc agccatctgt tgtttgcccc tcccccgtgc
      901 cttccttgac cctggaaggt gccactccca ctgtcctttc ctaataaaat gaggaaattg
      961 catcgcatt
//
