LOCUS       sequence-164714-         888 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Human PC1-CTp30 expression cassette lacking a Calmodulin binding 
            domain (delta C1) (632).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 888)
  AUTHORS   Doerr N, Wang Y, Kipp KR, Liu G, Benza JJ, Pletnev V, Pavlov TS, 
            Staruschenko A, Mohieldin AM, Takahashi M, Nauli SM, Weimbs T
  TITLE     Regulation of Polycystin-1 Function by Calmodulin Binding.
  JOURNAL   PLoS One. 2016 Aug 25;11(8):e0161525. doi: 
            10.1371/journal.pone.0161525. eCollection 2016.
  PUBMED    27560828
REFERENCE   2  (bases 1 to 888)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2016-08-25"; volume: "11(8):e0161525. doi"; pages: " 
            10.1371/journal.pone.0161525. eCollection 2016"
COMMENT     Sequence Label: pcDNA4/TO h-PKD1 FLS (4127-4303)-Myc/His
FEATURES             Location/Qualifiers
     source          1..888
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(32..61)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     primer_bind     complement(751..775)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    785..803
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    806..824
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        complement(826..877)
                     /label=CMVd2 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields moderately reduced expression 
                     relative to the full-length promoter"
     primer_bind     complement(855..875)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 ggtgatggtg atgatgaccg gtatgcatat tcagatcctc ttctgagatg agtttttgtt
       61 cgaatgggtg acctcgagcg gccgcagtgc tgctggggtg gaccttgttc ttggcccgaa
      121 ggggtgtcct gctggggcca gtggccaggt ccacaccccg actggcccgg gcaaggcggc
      181 tgggcagtgc tggccgcagg cccggggatg ggccacggga agatccggcg ggcgcccggc
      241 tgctcctgcg gccttgcagg ctgtgcagct gctgctccag ctggtagacg tcctctgtgg
      301 cctggttgag tcggtcaaac tgggtgagca gggcctcgaa cacggcttgg aggcgggagg
      361 gctcgggctc acaccttgtc cccagccggc ccaggctcac gctcagccca tccagctggc
      421 tggaggaggt ggaggggtgc gaggcatcgg agccagcgct gggtgggggc acatccgggg
      481 ataccttgga gcccctggag gagcgagagg gcagcggctc catcccttca aagcggactt
      541 tgtggcggaa ctccttgacc ttgctgaggc ccatccagag gcgcagcctg cgcaggaaca
      601 actccaccat ctcgtacatg aattccacca cactggacta gtggatccga gctcggtacc
      661 aagcttaagt ttaaacgcta gagtccggag gctggatcgg tcccggtgtc ttctatggag
      721 gtcaaaacag cgtggatggc gtctccaggc gatctgacgg ttcactaaac gagctcgtcg
      781 acgatctcta tcactgatag ggagatctct atcactgata gggagagctc tgcttatata
      841 gacctcccac cgtacacgcc taccgcccat ttgcgtcaat ggggcgga
//
