LOCUS       sequence-164720-         896 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Human PC1-CTp30 expression cassette lacking a Calmodulin binding 
            domain (delta C1) and with a point mutant that disrupts the second 
            binding domain (629).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 896)
  AUTHORS   Doerr N, Wang Y, Kipp KR, Liu G, Benza JJ, Pletnev V, Pavlov TS, 
            Staruschenko A, Mohieldin AM, Takahashi M, Nauli SM, Weimbs T
  TITLE     Regulation of Polycystin-1 Function by Calmodulin Binding.
  JOURNAL   PLoS One. 2016 Aug 25;11(8):e0161525. doi: 
            10.1371/journal.pone.0161525. eCollection 2016.
  PUBMED    27560828
REFERENCE   2  (bases 1 to 896)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2016-08-25"; volume: "11(8):e0161525. doi"; pages: " 
            10.1371/journal.pone.0161525. eCollection 2016"
COMMENT     Sequence Label: pcDNA4/TO h-PKD1 FLS (4127-4303) S4144D-Myc/His
FEATURES             Location/Qualifiers
     source          1..896
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    21..39
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     49..73
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             763..792
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             808..825
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(848..865)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
ORIGIN
        1 ccctatcagt gatagagatc tccctatcag tgatagagat cgtcgacgag ctcgtttagt
       61 gaaccgtcag atcgcctgga gacgccatcc acgctgtttt gacctccata gaagacaccg
      121 ggaccgatcc agcctccgga ctctagcgtt taaacttaag cttggtaccg agctcggatc
      181 cactagtcca gtgtggtgga attcatgtac gagatggtgg agttgttcct gcgcaggctg
      241 cgcctctgga tgggcctcga caaggtcaag gagttccgcc acaaagtccg ctttgaaggg
      301 atggagccgc tgccctctcg ctcctccagg ggctccaagg tatccccgga tgtgccccca
      361 cccagcgctg gctccgatgc ctcgcacccc tccacctcct ccagccagct ggatgggctg
      421 agcgtgagcc tgggccggct ggggacaagg tgtgagcccg agccctcccg cctccaagcc
      481 gtgttcgagg ccctgctcac ccagtttgac cgactcaacc aggccacaga ggacgtctac
      541 cagctggagc agcagctgca cagcctgcaa ggccgcagga gcagccgggc gcccgccgga
      601 tcttcccgtg gcccatcccc gggcctgcgg ccagcactgc ccagccgcct tgcccgggcc
      661 agtcggggtg tggacctggc cactggcccc agcaggacac cccttcgggc caagaacaag
      721 gtccacccca gcagcactgc ggccgctcga ggtcacccat tcgaacaaaa actcatctca
      781 gaagaggatc tgaatatgca taccggtcat catcaccatc accattgagt ttaaacccgc
      841 tgatcagcct cgactgtgcc ttctagttgc cagccatctg ttgtttgccc ctcccc
//
