LOCUS       sequence-164724-         909 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Human PC1-CTp30 expression cassette with a point mutant that 
            disrupts the second calmodulin binding domain (514).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 909)
  AUTHORS   Doerr N, Wang Y, Kipp KR, Liu G, Benza JJ, Pletnev V, Pavlov TS, 
            Staruschenko A, Mohieldin AM, Takahashi M, Nauli SM, Weimbs T
  TITLE     Regulation of Polycystin-1 Function by Calmodulin Binding.
  JOURNAL   PLoS One. 2016 Aug 25;11(8):e0161525. doi: 
            10.1371/journal.pone.0161525. eCollection 2016.
  PUBMED    27560828
REFERENCE   2  (bases 1 to 909)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2016-08-25"; volume: "11(8):e0161525. doi"; pages: " 
            10.1371/journal.pone.0161525. eCollection 2016"
COMMENT     Sequence Label: pcDNA4/TO h-PKD1 FLS S4144D-Myc/His
FEATURES             Location/Qualifiers
     source          1..909
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    8..26
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    29..47
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     57..81
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             838..867
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             883..900
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 agagctctcc ctatcagtga tagagatctc cctatcagtg atagagatcg tcgacgagct
       61 cgtttagtga accgtcagat cgcctggaga cgccatccac gctgttttga cctccataga
      121 agacaccggg accgatccag cctccggact ctagcgttta aacttaagct tggtaccgag
      181 ctcggatcca ctagtccagt gtggtggaat tctgttatgg cccgctggcg ctaccacgct
      241 ttgcgtggag agctgtaccg gccggcctgg gagccccagg actacgagat ggtggagttg
      301 ttcctgcgca ggctgcgcct ctggatgggc ctcgacaagg tcaaggagtt ccgccacaaa
      361 gtccgctttg aagggatgga gccgctgccc tctcgctcct ccaggggctc caaggtatcc
      421 ccggatgtgc ccccacccag cgctggctcc gatgcctcgc acccctccac ctcctccagc
      481 cagctggatg ggctgagcgt gagcctgggc cggctgggga caaggtgtga gcccgagccc
      541 tcccgcctcc aagccgtgtt cgaggccctg ctcacccagt ttgaccgact caaccaggcc
      601 acagaggacg tctaccagct ggagcagcag ctgcacagcc tgcaaggccg caggagcagc
      661 cgggcgcccg ccggatcttc ccgtggccca tccccgggcc tgcggccagc actgcccagc
      721 cgccttgccc gggccagtcg gggtgtggac ctggccactg gccccagcag gacacccctt
      781 cgggccaaga acaaggtcca ccccagcagc actgcggccg ctcgaggtca cccattcgaa
      841 caaaaactca tctcagaaga ggatctgaat atgcataccg gtcatcatca ccatcaccat
      901 tgagtttaa
//
