LOCUS       sequence-164726-         946 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Human PC1-CTp30 expression cassette with a point mutant that 
            disrupts the second calmodulin binding domain (514).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 946)
  AUTHORS   Doerr N, Wang Y, Kipp KR, Liu G, Benza JJ, Pletnev V, Pavlov TS, 
            Staruschenko A, Mohieldin AM, Takahashi M, Nauli SM, Weimbs T
  TITLE     Regulation of Polycystin-1 Function by Calmodulin Binding.
  JOURNAL   PLoS One. 2016 Aug 25;11(8):e0161525. doi: 
            10.1371/journal.pone.0161525. eCollection 2016.
  PUBMED    27560828
REFERENCE   2  (bases 1 to 946)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2016-08-25"; volume: "11(8):e0161525. doi"; pages: " 
            10.1371/journal.pone.0161525. eCollection 2016"
COMMENT     Sequence Label: pcDNA4/TO h-PKD1 FLS S4144D-Myc/His
FEATURES             Location/Qualifiers
     source          1..946
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(33..62)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     primer_bind     complement(819..843)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    853..871
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    874..892
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        complement(894..945)
                     /label=CMVd2 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields moderately reduced expression 
                     relative to the full-length promoter"
     primer_bind     complement(923..943)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 tggtgatggt gatgatgacc ggtatgcata ttcagatcct cttctgagat gagtttttgt
       61 tcgaatgggt gacctcgagc ggccgcagtg ctgctggggt ggaccttgtt cttggcccga
      121 aggggtgtcc tgctggggcc agtggccagg tccacacccc gactggcccg ggcaaggcgg
      181 ctgggcagtg ctggccgcag gcccggggat gggccacggg aagatccggc gggcgcccgg
      241 ctgctcctgc ggccttgcag gctgtgcagc tgctgctcca gctggtagac gtcctctgtg
      301 gcctggttga gtcggtcaaa ctgggtgagc agggcctcga acacggcttg gaggcgggag
      361 ggctcgggct cacaccttgt ccccagccgg cccaggctca cgctcagccc atccagctgg
      421 ctggaggagg tggaggggtg cgaggcatcg gagccagcgc tgggtggggg cacatccggg
      481 gataccttgg agcccctgga ggagcgagag ggcagcggct ccatcccttc aaagcggact
      541 ttgtggcgga actccttgac cttgtcgagg cccatccaga ggcgcagcct gcgcaggaac
      601 aactccacca tctcgtagtc ctggggctcc caggccggcc ggtacagctc tccacgcaaa
      661 gcgtggtagc gccagcgggc cataacagaa ttccaccaca ctggactagt ggatccgagc
      721 tcggtaccaa gcttaagttt aaacgctaga gtccggaggc tggatcggtc ccggtgtctt
      781 ctatggaggt caaaacagcg tggatggcgt ctccaggcga tctgacggtt cactaaacga
      841 gctcgtcgac gatctctatc actgataggg agatctctat cactgatagg gagagctctg
      901 cttatataga cctcccaccg tacacgccta ccgcccattt gcgtca
//
