LOCUS       sequence-164736-         945 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  for secretion of N-terminal OsmY, deca-histidine, SUMO tagged 
            protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 945)
  AUTHORS   Bhardwaj G, Mulligan VK, Bahl CD, Gilmore JM, Harvey PJ, Cheneval O,
            Buchko GW, Pulavarti SV, Kaas Q, Eletsky A, Huang PS, Johnsen WA, 
            Greisen PJ, Rocklin GJ, Song Y, Linsky TW, Watkins A, Rettie SA, Xu 
            X, Carter LP, Bonneau R, Olson JM, Coutsias E, Correnti CE, 
            Szyperski T, Craik DJ, Baker D
  TITLE     Accurate de novo design of hyperstable constrained peptides.
  JOURNAL   Nature. 2016 Sep 14. doi: 10.1038/nature19791.
  PUBMED    27626386
REFERENCE   2  (bases 1 to 945)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2016 Sep 14. doi: 10.1038/nature19791."
COMMENT     Sequence Label: pCDB180
FEATURES             Location/Qualifiers
     source          1..945
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             15..37
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             654..683
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
ORIGIN
        1 cctctagaaa taattttgtt taactttaag aaggagatat acatatgact atgacaagac
       61 tgaagatttc gaaaactctg ctggctgtaa tgttgacctc tgccgtcgcg accggctctg
      121 cctacgcgga aaacaacgcg cagactacca atgaaagcgc agggcaaaaa gtcgatagct
      181 ctatgaataa agtcggtaat ttcatggatg acagcgccat caccgcgaaa gtgaaggcgg
      241 ccctggtgga tcatgacaac atcaagagca ccgatatctc tgtaaaaacc gatcaaaaag
      301 tcgtgaccct gagcggtttc gttgaaagcc aggcccaggc cgaagaggca gtgaaagtgg
      361 cgaaaggcgt tgaaggggtg acctctgtca gcgacaaact gcacgttcgc gacgctaaag
      421 aaggctcggt gaagggctac gcgggtgaca ccgccaccac cagtgaaatc aaagccaaac
      481 tgctggcgga cgatatcgtc ccttcccgtc atgtgaaagt tgaaaccacc gacggcgtgg
      541 ttcagctctc cggtaccgtc gattctcagg cacaaagtga ccgtgctgaa agtatcgcca
      601 aagcggtaga tggtgtgaaa agcgttaaaa atgatctgaa aactaagatg ggtcaccacc
      661 accaccacca ccaccaccac cacggtagcc tgcaagattc ggaagtcaac caagaagcaa
      721 agccggaagt caagccggaa gtgaaaccgg aaacccatat taacctgaaa gttagtgacg
      781 gcagctctga aattttcttt aagatcaaaa agaccacgcc gctgcgtcgc ctgatggaag
      841 cgtttgccaa acgtcagggc aaggaaatgg atagcctgcg tttcctgtat gacggtattc
      901 gcatccaggc agatcaagcg ccggaagacc tggacatgga agaca
//
