LOCUS       sequence-164737-         847 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  for secretion of N-terminal OsmY, deca-histidine, SUMO tagged 
            protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 847)
  AUTHORS   Bhardwaj G, Mulligan VK, Bahl CD, Gilmore JM, Harvey PJ, Cheneval O,
            Buchko GW, Pulavarti SV, Kaas Q, Eletsky A, Huang PS, Johnsen WA, 
            Greisen PJ, Rocklin GJ, Song Y, Linsky TW, Watkins A, Rettie SA, Xu 
            X, Carter LP, Bonneau R, Olson JM, Coutsias E, Correnti CE, 
            Szyperski T, Craik DJ, Baker D
  TITLE     Accurate de novo design of hyperstable constrained peptides.
  JOURNAL   Nature. 2016 Sep 14. doi: 10.1038/nature19791.
  PUBMED    27626386
REFERENCE   2  (bases 1 to 847)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2016 Sep 14. doi: 10.1038/nature19791."
COMMENT     Sequence Label: pCDB180
FEATURES             Location/Qualifiers
     source          1..847
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(331..360)
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
ORIGIN
        1 ttcgggcttt gttagcagcc ggatcctcga gccgccaatc tgttcacggt gggcttcaat
       61 gatgtcgttg tcttccatgt ccaggtcttc cggcgcttga tctgcctgga tgcgaatacc
      121 gtcatacagg aaacgcaggc tatccatttc cttgccctga cgtttggcaa acgcttccat
      181 caggcgacgc agcggcgtgg tctttttgat cttaaagaaa atttcagagc tgccgtcact
      241 aactttcagg ttaatatggg tttccggttt cacttccggc ttgacttccg gctttgcttc
      301 ttggttgact tccgaatctt gcaggctacc gtggtggtgg tggtggtggt ggtggtggtg
      361 acccatctta gttttcagat catttttaac gcttttcaca ccatctaccg ctttggcgat
      421 actttcagca cggtcacttt gtgcctgaga atcgacggta ccggagagct gaaccacgcc
      481 gtcggtggtt tcaactttca catgacggga agggacgata tcgtccgcca gcagtttggc
      541 tttgatttca ctggtggtgg cggtgtcacc cgcgtagccc ttcaccgagc cttctttagc
      601 gtcgcgaacg tgcagtttgt cgctgacaga ggtcacccct tcaacgcctt tcgccacttt
      661 cactgcctct tcggcctggg cctggctttc aacgaaaccg ctcagggtca cgactttttg
      721 atcggttttt acagagatat cggtgctctt gatgttgtca tgatccacca gggccgcctt
      781 cactttcgcg gtgatggcgc tgtcatccat gaaattaccg actttattca tagagctatc
      841 gactttt
//
