LOCUS       sequence-164741-         969 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 969)
  AUTHORS   Schirmer EC, Homann OR, Kowal AS, Lindquist S
  TITLE     Dominant gain-of-function mutations in Hsp104p reveal crucial roles 
            for the middle region.
  JOURNAL   Mol Biol Cell 2004 May;15(5):2061-72. Epub 2004 Feb 20.
  PUBMED    14978213
REFERENCE   2  (bases 1 to 969)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Biol 
            Cell"; date: "2004-05"; volume: "15(5)"; pages: "2061-72. Epub 2004 
            Feb 20"
COMMENT     Sequence Label: CDC12(WT)
FEATURES             Location/Qualifiers
     source          1..969
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     6..25
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        6..24
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 aattgtaata cgactcacta tagggcgaat tggagctcca ccgcggtggc ggccgctcta
       61 gatatgtgcg tattgctgcg acaaaagaag agacaaagag ataggcgttg aaattgacga
      121 gacaaagagg aagacattaa ttaatcatca ttttaaatgg gattttttta cttgcaagct
      181 tttgacctgc tcttctagtt tttttacctt tccttgaatt tcttccaagt caccgttcag
      241 tctgattctt tcgttaacaa tgttttgctc ccattgtcta aatctttgct cctcagcttt
      301 gacttggtca gtgaaatact tcttcaatgc attttcttct tctttgtatc taggattgtg
      361 tgacaatttc cttgctggtg caattgctgg aagagtgaaa tcttcattcc cctccccggt
      421 gttctcgtga ccctcaagtc ttaatcttct gtatgtttcg taatgcattt cttgggtggt
      481 agaaatcaaa tctaatagat aagtcctcag taacagggct ctgagcttac ggaaatcaca
      541 atgagaatcg ttttcgattt ccacaagtcc ccaaggatac ttccttgcaa ctacctgagt
      601 gccttgccca ttgtcaaatt ttttttcaga ccctactatg gcaaatggca ttgcttctat
      661 caactgtctt gcatgttcaa cagcggcaga atctggatta ctgccacttt tggcatcttc
      721 cttagaatca gcgtctaatg gaggcgtgaa aatacgaatt tcttgagctt ctattacttg
      781 cctgattctg gacttgaact gttgtaattc ttgggcagtc aatgtatcag ctttcgcaat
      841 aactggaatc agattggctc tggttgacaa tctcttcata gtctcaatgt caattggctt
      901 taaaccatgt ccagtgggcc tgatgaagta cagaacggca tgaactctca agtcaaattt
      961 cttagtacg
//
