LOCUS       sequence-164742-         996 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  for secretion of N-terminal OsmY, deca-histidine, SUMO tagged 
            gEEH_04.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 996)
  AUTHORS   Bhardwaj G, Mulligan VK, Bahl CD, Gilmore JM, Harvey PJ, Cheneval O,
            Buchko GW, Pulavarti SV, Kaas Q, Eletsky A, Huang PS, Johnsen WA, 
            Greisen PJ, Rocklin GJ, Song Y, Linsky TW, Watkins A, Rettie SA, Xu 
            X, Carter LP, Bonneau R, Olson JM, Coutsias E, Correnti CE, 
            Szyperski T, Craik DJ, Baker D
  TITLE     Accurate de novo design of hyperstable constrained peptides.
  JOURNAL   Nature. 2016 Sep 14. doi: 10.1038/nature19791.
  PUBMED    27626386
REFERENCE   2  (bases 1 to 996)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2016 Sep 14. doi: 10.1038/nature19791."
COMMENT     Sequence Label: pCDB185
FEATURES             Location/Qualifiers
     source          1..996
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             3..25
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             642..671
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
ORIGIN
        1 attttgttta actttaagaa ggagatatac atatgactat gacaagactg aagatttcga
       61 aaactctgct ggctgtaatg ttgacctctg ccgtcgcgac cggctctgcc tacgcggaaa
      121 acaacgcgca gactaccaat gaaagcgcag ggcaaaaagt cgatagctct atgaataaag
      181 tcggtaattt catggatgac agcgccatca ccgcgaaagt gaaggcggcc ctggtggatc
      241 atgacaacat caagagcacc gatatctctg taaaaaccga tcaaaaagtc gtgaccctga
      301 gcggtttcgt tgaaagccag gcccaggccg aagaggcagt gaaagtggcg aaaggcgttg
      361 aaggggtgac ctctgtcagc gacaaactgc acgttcgcga cgctaaagaa ggctcggtga
      421 agggctacgc gggtgacacc gccaccacca gtgaaatcaa agccaaactg ctggcggacg
      481 atatcgtccc ttcccgtcat gtgaaagttg aaaccaccga cggcgtggtt cagctctccg
      541 gtaccgtcga ttctcaggca caaagtgacc gtgctgaaag tatcgccaaa gcggtagatg
      601 gtgtgaaaag cgttaaaaat gatctgaaaa ctaagatggg tcaccaccac caccaccacc
      661 accaccacca cggtagcctg caagattcgg aagtcaacca agaagcaaag ccggaagtca
      721 agccggaagt gaaaccggaa acccatatta acctgaaagt tagtgacggc agctctgaaa
      781 ttttctttaa gatcaaaaag accacgccgc tgcgtcgcct gatggaagcg tttgccaaac
      841 gtcagggcaa ggaaatggat agcctgcgtt tcctgtatga cggtattcgc atccaggcag
      901 atcaagcgcc ggaagacctg gacatggaag acaacgacat cattgaagcc caccgtgaac
      961 agattggcgg ccagtgctac accttccgtt ctgaat
//
