LOCUS       sequence-164749-         648 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  for secretion of N-terminal OsmY, deca-histidine, SUMO tagged 
            gEEH_04.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 648)
  AUTHORS   Bhardwaj G, Mulligan VK, Bahl CD, Gilmore JM, Harvey PJ, Cheneval O,
            Buchko GW, Pulavarti SV, Kaas Q, Eletsky A, Huang PS, Johnsen WA, 
            Greisen PJ, Rocklin GJ, Song Y, Linsky TW, Watkins A, Rettie SA, Xu 
            X, Carter LP, Bonneau R, Olson JM, Coutsias E, Correnti CE, 
            Szyperski T, Craik DJ, Baker D
  TITLE     Accurate de novo design of hyperstable constrained peptides.
  JOURNAL   Nature. 2016 Sep 14. doi: 10.1038/nature19791.
  PUBMED    27626386
REFERENCE   2  (bases 1 to 648)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2016 Sep 14. doi: 10.1038/nature19791."
COMMENT     Sequence Label: pCDB185
FEATURES             Location/Qualifiers
     source          1..648
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(449..478)
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
ORIGIN
        1 ctttcgggct ttgttagcag ccggatcctc attacttacg gcactcttct tctttggtac
       61 gacgcgcttc tttttcaact tcttccgggt tcggacggca aacggtgaat tctttgttgg
      121 tgcattcaga acggaaggtg tagcactggc cgccaatctg ttcacggtgg gcttcaatga
      181 tgtcgttgtc ttccatgtcc aggtcttccg gcgcttgatc tgcctggatg cgaataccgt
      241 catacaggaa acgcaggcta tccatttcct tgccctgacg tttggcaaac gcttccatca
      301 ggcgacgcag cggcgtggtc tttttgatct taaagaaaat ttcagagctg ccgtcactaa
      361 ctttcaggtt aatatgggtt tccggtttca cttccggctt gacttccggc tttgcttctt
      421 ggttgacttc cgaatcttgc aggctaccgt ggtggtggtg gtggtggtgg tggtggtgac
      481 ccatcttagt tttcagatca tttttaacgc ttttcacacc atctaccgct ttggcgatac
      541 tttcagcacg gtcactttgt gcctgagaat cgacggtacc ggagagctga accacgccgt
      601 cggtggtttc aactttcaca tgacgggaag ggacgatatc gtccgcca
//
