LOCUS       sequence-164754-         938 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  for secretion of N-terminal OsmY, deca-histidine, SUMO tagged 
            gEEHE_02.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 938)
  AUTHORS   Bhardwaj G, Mulligan VK, Bahl CD, Gilmore JM, Harvey PJ, Cheneval O,
            Buchko GW, Pulavarti SV, Kaas Q, Eletsky A, Huang PS, Johnsen WA, 
            Greisen PJ, Rocklin GJ, Song Y, Linsky TW, Watkins A, Rettie SA, Xu 
            X, Carter LP, Bonneau R, Olson JM, Coutsias E, Correnti CE, 
            Szyperski T, Craik DJ, Baker D
  TITLE     Accurate de novo design of hyperstable constrained peptides.
  JOURNAL   Nature. 2016 Sep 14. doi: 10.1038/nature19791.
  PUBMED    27626386
REFERENCE   2  (bases 1 to 938)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2016 Sep 14. doi: 10.1038/nature19791."
COMMENT     Sequence Label: pCDB289
FEATURES             Location/Qualifiers
     source          1..938
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             8..30
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             647..676
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
ORIGIN
        1 aaataatttt gtttaacttt aagaaggaga tatacatatg actatgacaa gactgaagat
       61 ttcgaaaact ctgctggctg taatgttgac ctctgccgtc gcgaccggct ctgcctacgc
      121 ggaaaacaac gcgcagacta ccaatgaaag cgcagggcaa aaagtcgata gctctatgaa
      181 taaagtcggt aatttcatgg atgacagcgc catcaccgcg aaagtgaagg cggccctggt
      241 ggatcatgac aacatcaaga gcaccgatat ctctgtaaaa accgatcaaa aagtcgtgac
      301 cctgagcggt ttcgttgaaa gccaggccca ggccgaagag gcagtgaaag tggcgaaagg
      361 cgttgaaggg gtgacctctg tcagcgacaa actgcacgtt cgcgacgcta aagaaggctc
      421 ggtgaagggc tacgcgggtg acaccgccac caccagtgaa atcaaagcca aactgctggc
      481 ggacgatatc gtcccttccc gtcatgtgaa agttgaaacc accgacggcg tggttcagct
      541 ctccggtacc gtcgattctc aggcacaaag tgaccgtgct gaaagtatcg ccaaagcggt
      601 agatggtgtg aaaagcgtta aaaatgatct gaaaactaag atgggtcacc accaccacca
      661 ccaccaccac caccacggta gcctgcaaga ttcggaagtc aaccaagaag caaagccgga
      721 agtcaagccg gaagtgaaac cggaaaccca tattaacctg aaagttagtg acggcagctc
      781 tgaaattttc tttaagatca aaaagaccac gccgctgcgt cgcctgatgg aagcgtttgc
      841 caaacgtcag ggcaaggaaa tggatagcct gcgtttcctg tatgacggta ttcgcatcca
      901 ggcagatcaa gcgccggaag acctggacat ggaagaca
//
