LOCUS       sequence-164762-         850 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  for secretion of N-terminal OsmY, deca-histidine, SUMO tagged 
            gEHE_06.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 850)
  AUTHORS   Bhardwaj G, Mulligan VK, Bahl CD, Gilmore JM, Harvey PJ, Cheneval O,
            Buchko GW, Pulavarti SV, Kaas Q, Eletsky A, Huang PS, Johnsen WA, 
            Greisen PJ, Rocklin GJ, Song Y, Linsky TW, Watkins A, Rettie SA, Xu 
            X, Carter LP, Bonneau R, Olson JM, Coutsias E, Correnti CE, 
            Szyperski T, Craik DJ, Baker D
  TITLE     Accurate de novo design of hyperstable constrained peptides.
  JOURNAL   Nature. 2016 Sep 14. doi: 10.1038/nature19791.
  PUBMED    27626386
REFERENCE   2  (bases 1 to 850)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2016 Sep 14. doi: 10.1038/nature19791."
COMMENT     Sequence Label: pCDB199
FEATURES             Location/Qualifiers
     source          1..850
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(438..467)
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
ORIGIN
        1 ttcgggcttt gttagcagcc ggatcctcat taggtttcgc actcaacttc cacttcgcaa
       61 ccggtacgac gagacagttc ttccgcgtat ttacggcatt cttcttcaga accacggtaa
      121 cgacgacgct gtttgcagcc gccaatctgt tcacggtggg cttcaatgat gtcgttgtct
      181 tccatgtcca ggtcttccgg cgcttgatct gcctggatgc gaataccgtc atacaggaaa
      241 cgcaggctat ccatttcctt gccctgacgt ttggcaaacg cttccatcag gcgacgcagc
      301 ggcgtggtct ttttgatctt aaagaaaatt tcagagctgc cgtcactaac tttcaggtta
      361 atatgggttt ccggtttcac ttccggcttg acttccggct ttgcttcttg gttgacttcc
      421 gaatcttgca ggctaccgtg gtggtggtgg tggtggtggt ggtggtgacc catcttagtt
      481 ttcagatcat ttttaacgct tttcacacca tctaccgctt tggcgatact ttcagcacgg
      541 tcactttgtg cctgagaatc gacggtaccg gagagctgaa ccacgccgtc ggtggtttca
      601 actttcacat gacgggaagg gacgatatcg tccgccagca gtttggcttt gatttcactg
      661 gtggtggcgg tgtcacccgc gtagcccttc accgagcctt ctttagcgtc gcgaacgtgc
      721 agtttgtcgc tgacagaggt caccccttca acgcctttcg ccactttcac tgcctcttcg
      781 gcctgggcct ggctttcaac gaaaccgctc agggtcacga ctttttgatc ggtttttaca
      841 gagatatcgg
//
