LOCUS       sequence-164768-         709 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  for secretion of N-terminal OsmY, deca-histidine, SUMO tagged 
            gEHEE_06.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 709)
  AUTHORS   Bhardwaj G, Mulligan VK, Bahl CD, Gilmore JM, Harvey PJ, Cheneval O,
            Buchko GW, Pulavarti SV, Kaas Q, Eletsky A, Huang PS, Johnsen WA, 
            Greisen PJ, Rocklin GJ, Song Y, Linsky TW, Watkins A, Rettie SA, Xu 
            X, Carter LP, Bonneau R, Olson JM, Coutsias E, Correnti CE, 
            Szyperski T, Craik DJ, Baker D
  TITLE     Accurate de novo design of hyperstable constrained peptides.
  JOURNAL   Nature. 2016 Sep 14. doi: 10.1038/nature19791.
  PUBMED    27626386
REFERENCE   2  (bases 1 to 709)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2016 Sep 14. doi: 10.1038/nature19791."
COMMENT     Sequence Label: pCDB181
FEATURES             Location/Qualifiers
     source          1..709
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(469..498)
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
ORIGIN
        1 tttcgggctt tgttagcagc cggatcctta tcagcaacgg atttcgcaaa cgtagcagtt
       61 accttcttta cggatctggt actggcagtt ctgacgttca ccacgttctt tgcattcacg
      121 acgaacacgt tcttcgtcct gaccgcaacg tttgtaacga cgttcttcgc cgccaatctg
      181 ttcacggtgg gcttcaatga tgtcgttgtc ttccatgtcc aggtcttccg gcgcttgatc
      241 tgcctggatg cgaataccgt catacaggaa acgcaggcta tccatttcct tgccctgacg
      301 tttggcaaac gcttccatca ggcgacgcag cggcgtggtc tttttgatct taaagaaaat
      361 ttcagagctg ccgtcactaa ctttcaggtt aatatgggtt tccggtttca cttccggctt
      421 gacttccggc tttgcttctt ggttgacttc cgaatcttgc aggctaccgt ggtggtggtg
      481 gtggtggtgg tggtggtgac ccatcttagt tttcagatca tttttaacgc ttttcacacc
      541 atctaccgct ttggcgatac tttcagcacg gtcactttgt gcctgagaat cgacggtacc
      601 ggagagctga accacgccgt cggtggtttc aactttcaca tgacgggaag ggacgatatc
      661 gtccgccagc agtttggctt tgatttcact ggtggtggcg gtgtcaccc
//
