LOCUS       sequence-164773-         602 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  for secretion of N-terminal OsmY, deca-histidine, SUMO tagged 
            gHEEE_02.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 602)
  AUTHORS   Bhardwaj G, Mulligan VK, Bahl CD, Gilmore JM, Harvey PJ, Cheneval O,
            Buchko GW, Pulavarti SV, Kaas Q, Eletsky A, Huang PS, Johnsen WA, 
            Greisen PJ, Rocklin GJ, Song Y, Linsky TW, Watkins A, Rettie SA, Xu 
            X, Carter LP, Bonneau R, Olson JM, Coutsias E, Correnti CE, 
            Szyperski T, Craik DJ, Baker D
  TITLE     Accurate de novo design of hyperstable constrained peptides.
  JOURNAL   Nature. 2016 Sep 14. doi: 10.1038/nature19791.
  PUBMED    27626386
REFERENCE   2  (bases 1 to 602)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2016 Sep 14. doi: 10.1038/nature19791."
COMMENT     Sequence Label: pCDB294
FEATURES             Location/Qualifiers
     source          1..602
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(456..485)
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
ORIGIN
        1 ttcgggcttt gttagcagcc ggatcctcat tagcacaggg tgtatttggt gtcagaacag
       61 cgaacttcaa cgcagtggtt agattcgtcg caacgcactt cgcagttttt gaatttcttt
      121 ttcatttcgg tgcatttctt acgggtttcc tgagagccgc caatctgttc acggtgggct
      181 tcaatgatgt cgttgtcttc catgtccagg tcttccggcg cttgatctgc ctggatgcga
      241 ataccgtcat acaggaaacg caggctatcc atttccttgc cctgacgttt ggcaaacgct
      301 tccatcaggc gacgcagcgg cgtggtcttt ttgatcttaa agaaaatttc agagctgccg
      361 tcactaactt tcaggttaat atgggtttcc ggtttcactt ccggcttgac ttccggcttt
      421 gcttcttggt tgacttccga atcttgcagg ctaccgtggt ggtggtggtg gtggtggtgg
      481 tggtgaccca tcttagtttt cagatcattt ttaacgcttt tcacaccatc taccgctttg
      541 gcgatacttt cagcacggtc actttgtgcc tgagaatcga cggtaccgga gagctgaacc
      601 ac
//
