LOCUS       sequence-164774-        1010 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  for secretion of N-terminal OsmY, deca-histidine, SUMO tagged 
            gHEEE_02.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1010)
  AUTHORS   Bhardwaj G, Mulligan VK, Bahl CD, Gilmore JM, Harvey PJ, Cheneval O,
            Buchko GW, Pulavarti SV, Kaas Q, Eletsky A, Huang PS, Johnsen WA, 
            Greisen PJ, Rocklin GJ, Song Y, Linsky TW, Watkins A, Rettie SA, Xu 
            X, Carter LP, Bonneau R, Olson JM, Coutsias E, Correnti CE, 
            Szyperski T, Craik DJ, Baker D
  TITLE     Accurate de novo design of hyperstable constrained peptides.
  JOURNAL   Nature. 2016 Sep 14. doi: 10.1038/nature19791.
  PUBMED    27626386
REFERENCE   2  (bases 1 to 1010)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2016 Sep 14. doi: 10.1038/nature19791."
COMMENT     Sequence Label: pCDB294
FEATURES             Location/Qualifiers
     source          1..1010
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             7..29
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             646..675
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
ORIGIN
        1 aataattttg tttaacttta agaaggagat atacatatga ctatgacaag actgaagatt
       61 tcgaaaactc tgctggctgt aatgttgacc tctgccgtcg cgaccggctc tgcctacgcg
      121 gaaaacaacg cgcagactac caatgaaagc gcagggcaaa aagtcgatag ctctatgaat
      181 aaagtcggta atttcatgga tgacagcgcc atcaccgcga aagtgaaggc ggccctggtg
      241 gatcatgaca acatcaagag caccgatatc tctgtaaaaa ccgatcaaaa agtcgtgacc
      301 ctgagcggtt tcgttgaaag ccaggcccag gccgaagagg cagtgaaagt ggcgaaaggc
      361 gttgaagggg tgacctctgt cagcgacaaa ctgcacgttc gcgacgctaa agaaggctcg
      421 gtgaagggct acgcgggtga caccgccacc accagtgaaa tcaaagccaa actgctggcg
      481 gacgatatcg tcccttcccg tcatgtgaaa gttgaaacca ccgacggcgt ggttcagctc
      541 tccggtaccg tcgattctca ggcacaaagt gaccgtgctg aaagtatcgc caaagcggta
      601 gatggtgtga aaagcgttaa aaatgatctg aaaactaaga tgggtcacca ccaccaccac
      661 caccaccacc accacggtag cctgcaagat tcggaagtca accaagaagc aaagccggaa
      721 gtcaagccgg aagtgaaacc ggaaacccat attaacctga aagttagtga cggcagctct
      781 gaaattttct ttaagatcaa aaagaccacg ccgctgcgtc gcctgatgga agcgtttgcc
      841 aaacgtcagg gcaaggaaat ggatagcctg cgtttcctgt atgacggtat tcgcatccag
      901 gcagatcaag cgccggaaga cctggacatg gaagacaacg acatcattga agcccaccgt
      961 gaacagattg gcggctctca ggaaacccgt aagaaatgca ccgaaatgaa
//
