LOCUS       sequence-164779-         641 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  for secretion of N-terminal OsmY, deca-histidine, SUMO tagged 
            gHH_44.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 641)
  AUTHORS   Bhardwaj G, Mulligan VK, Bahl CD, Gilmore JM, Harvey PJ, Cheneval O,
            Buchko GW, Pulavarti SV, Kaas Q, Eletsky A, Huang PS, Johnsen WA, 
            Greisen PJ, Rocklin GJ, Song Y, Linsky TW, Watkins A, Rettie SA, Xu 
            X, Carter LP, Bonneau R, Olson JM, Coutsias E, Correnti CE, 
            Szyperski T, Craik DJ, Baker D
  TITLE     Accurate de novo design of hyperstable constrained peptides.
  JOURNAL   Nature. 2016 Sep 14. doi: 10.1038/nature19791.
  PUBMED    27626386
REFERENCE   2  (bases 1 to 641)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2016 Sep 14. doi: 10.1038/nature19791."
COMMENT     Sequence Label: pCDB278
FEATURES             Location/Qualifiers
     source          1..641
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(418..447)
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
ORIGIN
        1 tttcgggctt tgttagcagc cggatcctca ttacgcctgg cacttctcca gttttttctt
       61 gatttcgtcg ttcgggtttt tttccagttc tttacggata cgttcgcagt cttcagcgcc
      121 gccaatctgt tcacggtggg cttcaatgat gtcgttgtct tccatgtcca ggtcttccgg
      181 cgcttgatct gcctggatgc gaataccgtc atacaggaaa cgcaggctat ccatttcctt
      241 gccctgacgt ttggcaaacg cttccatcag gcgacgcagc ggcgtggtct ttttgatctt
      301 aaagaaaatt tcagagctgc cgtcactaac tttcaggtta atatgggttt ccggtttcac
      361 ttccggcttg acttccggct ttgcttcttg gttgacttcc gaatcttgca ggctaccgtg
      421 gtggtggtgg tggtggtggt ggtggtgacc catcttagtt ttcagatcat ttttaacgct
      481 tttcacacca tctaccgctt tggcgatact ttcagcacgg tcactttgtg cctgagaatc
      541 gacggtaccg gagagctgaa ccacgccgtc ggtggtttca actttcacat gacgggaagg
      601 gacgatatcg tccgccagca gtttggcttt gatttcactg g
//
