LOCUS       sequence-164783-         966 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  for secretion of N-terminal OsmY, deca-histidine, SUMO tagged 
            gHHH_06.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 966)
  AUTHORS   Bhardwaj G, Mulligan VK, Bahl CD, Gilmore JM, Harvey PJ, Cheneval O,
            Buchko GW, Pulavarti SV, Kaas Q, Eletsky A, Huang PS, Johnsen WA, 
            Greisen PJ, Rocklin GJ, Song Y, Linsky TW, Watkins A, Rettie SA, Xu 
            X, Carter LP, Bonneau R, Olson JM, Coutsias E, Correnti CE, 
            Szyperski T, Craik DJ, Baker D
  TITLE     Accurate de novo design of hyperstable constrained peptides.
  JOURNAL   Nature. 2016 Sep 14. doi: 10.1038/nature19791.
  PUBMED    27626386
REFERENCE   2  (bases 1 to 966)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2016 Sep 14. doi: 10.1038/nature19791."
COMMENT     Sequence Label: pCDB267
FEATURES             Location/Qualifiers
     source          1..966
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             16..38
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             655..684
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
ORIGIN
        1 ccctctagaa ataattttgt ttaactttaa gaaggagata tacatatgac tatgacaaga
       61 ctgaagattt cgaaaactct gctggctgta atgttgacct ctgccgtcgc gaccggctct
      121 gcctacgcgg aaaacaacgc gcagactacc aatgaaagcg cagggcaaaa agtcgatagc
      181 tctatgaata aagtcggtaa tttcatggat gacagcgcca tcaccgcgaa agtgaaggcg
      241 gccctggtgg atcatgacaa catcaagagc accgatatct ctgtaaaaac cgatcaaaaa
      301 gtcgtgaccc tgagcggttt cgttgaaagc caggcccagg ccgaagaggc agtgaaagtg
      361 gcgaaaggcg ttgaaggggt gacctctgtc agcgacaaac tgcacgttcg cgacgctaaa
      421 gaaggctcgg tgaagggcta cgcgggtgac accgccacca ccagtgaaat caaagccaaa
      481 ctgctggcgg acgatatcgt cccttcccgt catgtgaaag ttgaaaccac cgacggcgtg
      541 gttcagctct ccggtaccgt cgattctcag gcacaaagtg accgtgctga aagtatcgcc
      601 aaagcggtag atggtgtgaa aagcgttaaa aatgatctga aaactaagat gggtcaccac
      661 caccaccacc accaccacca ccacggtagc ctgcaagatt cggaagtcaa ccaagaagca
      721 aagccggaag tcaagccgga agtgaaaccg gaaacccata ttaacctgaa agttagtgac
      781 ggcagctctg aaattttctt taagatcaaa aagaccacgc cgctgcgtcg cctgatggaa
      841 gcgtttgcca aacgtcaggg caaggaaatg gatagcctgc gtttcctgta tgacggtatt
      901 cgcatccagg cagatcaagc gccggaagac ctggacatgg aagacaacga catcattgaa
      961 gcccac
//
