LOCUS       sequence-164784-         722 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  for secretion of N-terminal OsmY, deca-histidine, SUMO tagged 
            gHHH_06.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 722)
  AUTHORS   Bhardwaj G, Mulligan VK, Bahl CD, Gilmore JM, Harvey PJ, Cheneval O,
            Buchko GW, Pulavarti SV, Kaas Q, Eletsky A, Huang PS, Johnsen WA, 
            Greisen PJ, Rocklin GJ, Song Y, Linsky TW, Watkins A, Rettie SA, Xu 
            X, Carter LP, Bonneau R, Olson JM, Coutsias E, Correnti CE, 
            Szyperski T, Craik DJ, Baker D
  TITLE     Accurate de novo design of hyperstable constrained peptides.
  JOURNAL   Nature. 2016 Sep 14. doi: 10.1038/nature19791.
  PUBMED    27626386
REFERENCE   2  (bases 1 to 722)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2016 Sep 14. doi: 10.1038/nature19791."
COMMENT     Sequence Label: pCDB267
FEATURES             Location/Qualifiers
     source          1..722
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(465..494)
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
ORIGIN
        1 ttcgggcttt gttagcagcc ggatcctcat taagattcgc agttacgcgc catacgttcc
       61 gcatcctcag aggtgccttc tttgcacatt ttttccagac gctgacggca ttcttcagac
      121 atacccagtt ttttcagacg ttctttcagg tcttcgcacg gagcgccgcc aatctgttca
      181 cggtgggctt caatgatgtc gttgtcttcc atgtccaggt cttccggcgc ttgatctgcc
      241 tggatgcgaa taccgtcata caggaaacgc aggctatcca tttccttgcc ctgacgtttg
      301 gcaaacgctt ccatcaggcg acgcagcggc gtggtctttt tgatcttaaa gaaaatttca
      361 gagctgccgt cactaacttt caggttaata tgggtttccg gtttcacttc cggcttgact
      421 tccggctttg cttcttggtt gacttccgaa tcttgcaggc taccgtggtg gtggtggtgg
      481 tggtggtggt ggtgacccat cttagttttc agatcatttt taacgctttt cacaccatct
      541 accgctttgg cgatactttc agcacggtca ctttgtgcct gagaatcgac ggtaccggag
      601 agctgaacca cgccgtcggt ggtttcaact ttcacatgac gggaagggac gatatcgtcc
      661 gccagcagtt tggctttgat ttcactggtg gtggcggtgt cacccgcgta gcccttcacc
      721 ga
//
