LOCUS       sequence-164790-         999 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentivirus vector. Expresses mutant Halotag-Cbx7 fusion proteins in 
            mammalian cells. Cbx7 deletion of amino acids 1–83..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 999)
  TITLE     pTRIPZ-MSC1-Halotag-Cbx2
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 999)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pTRIPZ (M)-HT-Cbx7-delta-(CD -ATL)
FEATURES             Location/Qualifiers
     source          1..999
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      2..11
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             8..898
                     /codon_start=1
                     /product="modified bacterial dehalogenase that forms 
                     covalent bonds with chloroalkane derivatives"
                     /label=HaloTag(R)
                     /translation="MAEIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPTSS
                     YVWRNIIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVI
                     HDWGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLII
                     DQNVFIEGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIVALV
                     EEYMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGPGLNLLQEDNPDL
                     IGSEIARWLSTLEISG"
ORIGIN
        1 ggccaccatg gcagaaatcg gtactggctt tccattcgac ccccattatg tggaagtcct
       61 gggcgagcgc atgcactacg tcgatgttgg tccgcgcgat ggcacccctg tgctgttcct
      121 gcacggtaac ccgacctcct cctacgtgtg gcgcaacatc atcccgcatg ttgcaccgac
      181 ccatcgctgc attgctccag acctgatcgg tatgggcaaa tccgacaaac cagacctggg
      241 ttatttcttc gacgaccacg tccgcttcat ggatgccttc atcgaagccc tgggtctgga
      301 agaggtcgtc ctggtcattc acgactgggg ctccgctctg ggtttccact gggccaagcg
      361 caatccagag cgcgtcaaag gtattgcatt tatggagttc atccgcccta tcccgacctg
      421 ggacgaatgg ccagaatttg cccgcgagac cttccaggcc ttccgcacca ccgacgtcgg
      481 ccgcaagctg atcatcgatc agaacgtttt tatcgagggt acgctgccga tgggtgtcgt
      541 ccgcccgctg actgaagtcg agatggacca ttaccgcgag ccgttcctga atcctgttga
      601 ccgcgagcca ctgtggcgct tcccaaacga gctgccaatc gccggtgagc cagcgaacat
      661 cgtcgcgctg gtcgaagaat acatggactg gctgcaccag tcccctgtcc cgaagctgct
      721 gttctggggc accccaggcg ttctgatccc accggccgaa gccgctcgcc tggccaaaag
      781 cctgcctaac tgcaaggctg tggacatcgg cccgggtctg aatctgctgc aagaagacaa
      841 cccggacctg atcggcagcg agatcgcgcg ctggctgtcg acgctcgaga tttccggcgc
      901 gaattcactt gctctgtaca gcatggacct gcggagctcc cacaaggcca agggcaagga
      961 gaagctctgc ttctccctga cgtgcccact cggcagcgg
//
